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Selected Publication:

Sinitsyna, ES; Walter, JG; Vlakh, EG; Stahl, F; Kasper, C; Tennikova, TB.
(2012): Macroporous methacrylate-based monoliths as platforms for DNA microarrays.
Talanta. 2012; 93(10):139-146 FullText FullText_BOKU

Abstract:
Macroporous monoliths with different surface functionalization (reactive groups) were utilized as platforms for DNA analysis in microarray format. The slides based on a copolymer glycidyl methacrylate-co-ethylene dimethacrylate (GMA-EDMA) have been chosen as well known and thoroughly studied standard. In particular, this material has been used at optimization of DNA microanalytical procedure. The concentration and pH of spotting solution, immobilization temperature and time, blocking agent and coupling reaction duration were selected as varied parameters. The efficiency of analysis performed on 3-D monolithic platforms was compared to that established for commercially available glass slides. As a practical example, a diagnostic test for detection of CFTR gene mutation was carried out. Additionally, the part of presented work was devoted to preparation of aptamer-based test-system that allowed successful and highly sensitive detection both of DNA and protein. (C) 2012 Elsevier B.V. All rights reserved.
Authors BOKU Wien:
Almeria Ciarra
Kasper Cornelia
Find related publications in this database (using NML MeSH Indexing)
Animals -
Aptamers, Nucleotide - metabolism
Cystic Fibrosis Transmembrane Conductance Regulator - genetics
Glass - chemistry
Methacrylates - chemistry
Mutation -
Oligonucleotide Array Sequence Analysis - methods
Oligonucleotide Probes - chemistry
Polymers - chemistry
Porosity -
Printing -
Surface Properties -



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