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Selected Publication:

Harrison, A; Binder, H; Buhot, A; Burden, CJ; Carlon, E; Gibas, C; Gamble, LJ; Halperin, A; Hooyberghs, J; Kreil, DP; Levicky, R; Noble, PA; Ott, A; Pettitt, BM; Tautz, D; Pozhitkov, AE.
(2013): Physico-chemical foundations underpinning microarray and next-generation sequencing experiments.
Nucleic Acids Res. 2013; 41(5):2779-2796 FullText FullText_BOKU

Hybridization of nucleic acids on solid surfaces is a key process involved in high-throughput technologies such as microarrays and, in some cases, next-generation sequencing (NGS). A physical understanding of the hybridization process helps to determine the accuracy of these technologies. The goal of a widespread research program is to develop reliable transformations between the raw signals reported by the technologies and individual molecular concentrations from an ensemble of nucleic acids. This research has inputs from many areas, from bioinformatics and biostatistics, to theoretical and experimental biochemistry and biophysics, to computer simulations. A group of leading researchers met in Ploen Germany in 2011 to discuss present knowledge and limitations of our physico-chemical understanding of high-throughput nucleic acid technologies. This meeting inspired us to write this summary, which provides an overview of the state-of-the-art approaches based on physicochemical foundation to modeling of the nucleic acids hybridization process on solid surfaces. In addition, practical application of current knowledge is emphasized.
Authors BOKU Wien:
Kreil David Philip
Find related publications in this database (using NML MeSH Indexing)
Algorithms -
Artifacts -
Base Pairing -
Calibration -
DNA - chemistry
DNA Probes - chemistry
High-Throughput Nucleotide Sequencing -
Humans -
Image Processing, Computer-Assisted -
Models, Biological -
Nucleic Acid Hybridization - methods
Oligonucleotide Array Sequence Analysis -
Surface Properties -
Thermodynamics -

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