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Selected Publication:

Roeker, S; Bohm, S; Diederichs, S; Bode, F; Quade, A; Korzhikov, V; van Griensven, M; Tennikova, TB; Kasper, C.
(2009): A Study on the Influence of Biocompatible Composites With Bioactive Ligands Toward their Effect on Cell Adhesion and Growth for the Application in Bone Tissue Engineering
J BIOMED MATER RES B. 2009; 91B(1): 153-162. FullText FullText_BOKU

The aim of this study was the transformation of the macroporous zirconium dioxide ceramic Sponceram (R) into a biomimetic composite material. To enhance the adhesion of cells and to induce their differentiation into osteoblasts poly-L-lysine and BMP-2 were coupled to polymers and copolymers based on 2-deoxy-N-methacrylamido-D-glucose (ox.p(MAG) and p(MVA)) used as spacer, which were adsorbed onto the ceramic surface. The development of the composite materials was validated step by step qualitatively and quantitatively. The bioactive potential of the composite materials was tested under static and dynamic conditions using an osteoblastic model cell line and human mesenchymal stem cells. Both composite materials showed potential to enhance the adhesion of cells in the first 10 days of their cultivation. One of the composite materials, namely Sponceram (R)/ox.p(MAG)-BMP-2, was tested into a rotating-bed bioreactor with regard to its osteogenic differentiation-inducing potential. Compared with Sponceram (R) modified with BMP-2 without a polymer spacer, it showed increased expression of osteogenic markers determined by PCR analysis. In summary, the in vitro testing of the developed composite materials demonstrated a promising potential for their application as biomimetic scaffold materials with controllable properties. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 91B: 153-162, 2009
Authors BOKU Wien:
Almeria Ciarra
Kasper Cornelia
Find related publications in this database (using NML MeSH Indexing)
3T3 Cells -
Adsorption -
Animals -
Biocompatible Materials - chemistry
Bone Morphogenetic Protein 2 - chemistry
Bone Substitutes - chemistry
Bone and Bones - cytology
Cell Adhesion - physiology
Cell Differentiation - physiology
Cells, Cultured -
Ceramics - chemistry
Glucose - chemistry
Humans -
Ligands -
Lysine - chemistry
Materials Testing -
Mesenchymal Stem Cells - cytology
Mice -
Molecular Structure -
Osteoblasts - cytology
Surface Properties -
Tissue Engineering - methods

Find related publications in this database (Keywords)
polymer spacer
composite material
bone tissue engineering
functionalized scaffolds
bioreactor cultivation

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