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Shephard, GS; Burger, HM; Gambacorta, L; Gong, YY; Krska, R; Rheeder, JP; Solfrizzo, M; Srey, C; Sulyok, M; Visconti, A; Warth, B; van der Westhuizen, L.
(2013): Multiple mycotoxin exposure determined by urinary biomarkers in rural subsistence farmers in the former Transkei, South Africa
FOOD CHEM TOXICOL. 2013; 62: 217-225. FullText FullText_BOKU

Abstract:
Subsistence farmers are exposed to a range of mycotoxins. This study applied novel urinary multi-mycotoxin LC-MS/MS methods to determine multiple exposure biomarkers in the high oesophageal cancer region, Transkei, South Africa. Fifty-three female participants donated part of their maize-based evening meal and first void morning urine, which was analysed both with sample clean-up (single and multi-biomarker) and by a xxxdilute-and-shootxxx multi-biomarker method. Results were corrected for recovery with LOD for not detected. A single biomarker method detected fumonisin B-1 (FB1) (87% incidence; mean standard deviation 0.342 +/- 0.466 ng/mg creatinine) and deoxynivalenol (100%; mean 20.4 +/- 49.4 ng/mg creat-inine) after hydrolysis with B-glucuronidase. The multi-biomarker xxxdilute-and-shootxxx method indicated deoxynivalenol-15-glucuronide was predominantly present. A multi-biomarker method with beta-glucuronidase and immunoaffinity clean-up determined zearalenone (100%; 0.529 +/- 1.60 ng/mg creatinine), FBI (96%; 1.52 +/- 2.17 ng/mg creatinine), alpha-zearalenol (92%; 0.614 +/- 1.91 ng/mg creatinine), deoxynivalenol (87%; 11.3 +/- 27.1 ng/mg creatinine), beta-zearalenol (75%; 0.702 +/- 2.95 ng/mg creatinine) and ochratoxin A (98%; 0.041 +/- 0.086 ng/mg creatinine). These demonstrate the value of multi-biomarker methods in measuring exposures in populations exposed to multiple mycotoxins. This is the first finding of urinary deoxynivalenol, zearalenone, their conjugates, ochratoxin A and zearalenols in Transkei. (C) 2013 Elsevier Ltd. All rights reserved.
Authors BOKU Wien:
Krska Rudolf
Sulyok Michael
Warth Benedikt
BOKU Gendermonitor:

Find related publications in this database (using NML MeSH Indexing)
Adult
Aged
Aged, 80 and over
Biomarkers/urine*
Environmental Exposure/analysis*
Farmers
Female
Food Contamination/analysis*
Fumonisins/urine
Humans
Middle Aged
Mycotoxins/analysis
Mycotoxins/toxicity*
Ochratoxins/urine
Rural Population
South Africa
Tandem Mass Spectrometry/methods
Trichothecenes/urine
Young Adult
Zea mays
Zearalenone/urine
Zeranol/analogs & derivatives
Zeranol/urine

Find related publications in this database (Keywords)
Biomarker
Fumonisin
Deoxynivalenol
Exposure
Mycotoxin
Zearalenone


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