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Hahn, I; Nagl, V; Schwartz-Zimmermann, HE; Varga, E; Schwarz, C; Slavik, V; Reisinger, N; Malachová, A; Cirlini, M; Generotti, S; Dall"Asta, C; Krska, R; Moll, WD; Berthiller, F; .
(2015): Effects of orally administered fumonisin B₁ (FB₁), partially hydrolysed FB₁, hydrolysed FB₁ and N-(1-deoxy-D-fructos-1-yl) FB₁ on the sphingolipid metabolism in rats.
Food Chem Toxicol. 2015; 76:11-18 FullText FullText_BOKU

Abstract:
Fumonisin B-1 (FB1) is a Fusarium mycotoxin frequently occurring in maize-based food and feed. Alkaline processing like nixtamalisation of maize generates partially and fully hydrolysed FB1 (pHFB(1) and HFB1) and thermal treatment in the presence of reducing sugars leads to formation of N-(1-deoxy-D-fructos-1-y1) fumonisin B-1 (NDF). The toxicity of these metabolites, in particular their effect on the sphingolipid metabolism, is either unknown or discussed controversially. We produced high purity FB1, pHFB(1)a+b, HFB1 and NDF and fed them to male Sprague Dawley rats for three weeks. Once a week, urine and faeces samples were collected over 24 h and analysed for fumonisin metabolites as well as for the sphinganine (Sa) to sphingosine (So) ratio by validated LC-MS/MS based methods. While the latter was significantly increased in the FB1 positive control group, the Sa/So ratios of the partially and fully hydrolysed fumonisins were indifferent from the negative control group. Although NDF was partly cleaved during digestion, the liberated amounts of FB1 did not raise the Sa/So ratio. These results show that the investigated alkaline and thermal processing products of FB1 were, at the tested concentrations, non-toxic for rats, and suggest that according food processing can reduce fumonisin toxicity for humans. (C) 2014 Elsevier Ltd. All rights reserved.
Authors BOKU Wien:
Berthiller Franz
Hahn Irene
Jedlaucnik Veronika
Krska Rudolf
Nagl Veronika
Schwartz-Zimmermann Heidi Elisabeth
Schwarz Christiane
Varga Elisabeth
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Find related publications in this database (Keywords)
Mycotoxins
Rodents
Biomarkers
ADME
Fumonisin metabolism
LC-MS/MS


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