Chromatin and transcriptional control in fungi
Abstract
The aim of this project is to ecipher the molecular mechanisms by which LaeA-dependent light regulation is connected to fungal secondary metabolite production and differentiation. Recently, it was also shown that the LaeA orthologue of Trichoderma (LAE1) controls cellulase biosynthesis and biocontrol abilities in Trichoderma [. We want to identify interactions of proteins within large complexes using either a double-tag protein purification method and thus alleviating the need to perform mass spectrometric techniques or using sequential yeast-two-hybrid screens to define complex interactions. We will use aforementioned techniques for studying the interaction partners of LaeA/LAE1 as well as VEL1 [most recently vel1 was also identified as the other essential components for cellulase synthesis in Trichoderma reesei which interacts physically with LAE1, and XYR1. Moreover, we will also further study the molecular mechanism of action of the putative methyltransferase LaeA/LAE1 in the model organism Aspergillus nidulans and in the industrially important fungus T. reesei by applying cutting-edge molecular and biochemical methods and likewise utilizing various bioinformatics pipelines which have been already established in the Braus laboratory and are applied routinely there.
keywords LaeA gene regulation epigenetics
Publikationen
Project staff
Joseph Strauss
Univ.Prof. Mag.rer.nat. Dr.rer.nat. Joseph Strauss
joseph.strauss@boku.ac.at
Tel: +43 1 47654-94420
Project Leader
01.10.2018 - 08.10.2018