stabilisation of redox enzymes for use in onplantable biosensors
Abstract
Modern medical diagnostics is moving towards continuous measurements, which provide data in real time. An example for this can be biosensors that are implanted in the skin and measure blood glucose levels. Currently, stability of enzyme-based sensors is limited by several days, yet very stable enzymes could extend this to several weeks or even months. Cellobiose dehydrogenase (CDH) is a redox enzyme that based on its uniques architecture enables direct electron transfer to electrodes. Asa flavocyctochrome, CDH is a very stable enzyme, and its inactivation mechanisms are not explored to date. It could be shown though that hydrogen peroxide, which can be fored as a byproduct, results in inactivation, similar to glucose oxidase, which is the most frequently ezyme in blood glucose sensors. The project STARELIS will elucidate the inactivation menchanisms relevant for CDH and thus should serve as the basis for the development of stable, implantable glucose sensors. By combining various biochemical and biophysical analytical methods together with enzyme engineering and molecular modeling the project is aiming at elucidating the effect of oxidative stress on CDH.
keywords cellobiose dehydrogenase flavocyctochrome glucse sensor implantable enzyme engineering
Publikationen
Project staff
Dietmar Haltrich
Univ.Prof. Dipl.-Ing. Dr.techn. Dietmar Haltrich
dietmar.haltrich@boku.ac.at
Tel: +43 1 47654-75211
Project Leader
01.08.2018 - 31.12.2021
BOKU partners
External partners
DirectSens
Dr. Roman Kittl
partner