Continuous three dimensional cultivation of mesenchymal stem cells
Abstract
Mesenchymal stem cells (MSCs) are important candidates for cell-based therapies in regenerative medicine. MSCs are isolated from donor tissue and then propagated in vitro on 2D plastic surfaces. These traditional culture formats do not match the physiological in vivo situation. MSCs cultured under 3D conditions (e.g., as aggregates) show much higher therapeutic potential. Within the framework of this project, a bioprocess is being developed which enables the isolation and propagation of MSCs continuously under three-dimensional conditions. For this, donor tissue is embedded in a hydrogel, whereupon MSCs migrate from the tissue into the hydrogel. These cells are then propagated as 3D aggregates in a stirred tank bioreactor. This process avoids repetitive passenger traffic, which among other things leads to damage to the surface markers and unwanted genetic changes.
Publikationen
Project staff
Dominik Egger
Dr.rer.nat. Dominik Egger MSc.
dominik.egger@boku.ac.at
Tel: +43 1 47654-79893
Project Leader
01.07.2019 - 31.03.2021