Identification of critical process parameter for the production of extracellular vesicles
Abstract
For the therapy of degenerative diseases as well as the stimulation of regenerative processes extracellular vesicles come to the fore. In the course of unraveling the mechanisms of stem cell therapies it was discovered that an important factor of signal transduction for tissue regeneration was stem cell produced extracellular vesicles (EV). EVs transfer biologically active molecules from stem cells to diseased cells transmitting therapeutic effects. If EVs can be isolated and purified, their regenerative effects can be exploited without the risks connected to stem cell therapy. The cell-free treatment with EVs is a promising alternative to cell therapy being more effective, safer, and cheaper. Though, as the effectivity of stem cell therapy is dependent on the condition of the cells, so is the effectivity of the EVs dependent on their cargo. The culture conditions of the EV-producing cells is directly affecting the EV cargo and respectively their therapeutic effect. Thus, aim of this study is the investigation of different culture conditions on the cargo and effectivity of EVs.