Cell and tissue culture for resistance breeding in selected genotypes of olive (Olea sp.)
Abstract
Plant diseases pose a major thread to global food security. The major production constraints in olive cultivation arise from the occurrence of specific emergent pests and diseases leading to significant yield losses, such as the Olive Quick Decline Syndrome disease (OQDS), caused by the highly virulent bacterium Xylella fastidiosa. OQDS currently represents one of the most significant emerging plant pathogens at global scales. PBU will provide axenic tissue cultures at different levels of organization, from shoot cultures from axillary and adventitious buds, somatic embryogenesis from juvenile and adult explants, including single cell regeneration, allowing to reach the required sample size for screening within a reasonable time frame. Mutations will be induced either by Gamma- and X-ray together with PBGL or by CRISPR-Cas. PBU will attempt a molecular validation of mutants to provide a valid advice on how to generate, screen and recover interesting mutant genotypes of olive (Olea sp.) after irradiation using enabling techniques without the need for time consuming chimera dissolution processes.
Publikationen
Project staff
Margit Laimer
Ao.Univ.Prof. Dr.phil. Margit Laimer
margit.laimer@boku.ac.at
Tel: +43 1 47654-79010
Project Leader
04.05.2021 - 03.05.2026