Digital PCR assays for the detection of pathogens from field samples

The detection and quantification of plant pathogens in field samples are critical for monitoring and controlling plant diseases in agricultural environments. This project focuses on the development and optimization of digital PCR (dPCR) assays for the precise and sensitive detection of plant pathogens from field-collected samples. Digital PCR offers several advantages over traditional PCR methods, including absolute quantification without the need for standard curves, increased sensitivity and specificity and greater tolerance to PCR inhibitors. By leveraging these benefits, our assays aim to provide reliable and rapid diagnostics for a range of plant pathogens relevant in the agricultural context.