Enzymology of xylose utilization in yeast.
Abstract
The specificity and substrate recognition of ALR and XDH will be probed by steady-state kinetic analysis using a series of mono- and disubstituted analogs of D-glucose, D-xylose, D-fructose and D-xylulose in which the hydroxyl groups are substituted by hydrogen, fluorine or azide. The nature and the relative importance of the contribution to transition state binding of each hydroxyl group in the sugar substrate - an aspect of the catalytic mechanism of ALR and XDH that has not been investigated - will thus be evaluated systematically. Hence, a fingerprint of the active site environment of both enzymes will be obtained. The synthesis of substituted analogs in substrate quantities and high yields will be accomplished by novel chemo-enzymatic methods and highlights the interdisciplinary approach of the project.
keywords bonding energy catalysis
Publikationen
Project staff
BOKU partners
External partners
Technical University Graz, Institute of Organic Chemistry
Univ.Doz. Dipl.-Ing. Dr. Arnold Stütz
partner