S-layer supported Langmuir-Blodgett films and biomembranes. (S7205-MOB)
Abstract
The fabrication of biomimetic membranes is essential for the investigation of structure-function relationships of biologically functional molecules. Since the concept of an S-layer supported lipid membran has already been optimized by nature in the cell envelope of archaebacteria this research project is concentrated on the recrystallization of S-layers on phospholipid- and tetraetherlipid films. The functionalization of such S-layer stabilized membranes is performed by integrating functional molecules such as carriers, ion channels or proton pumps. The fabrication and application of such composite membranes might lead to a key technology in the investigation of membrane associated or integrated functional molecules.
biomimetics patch clamp lipid membranes protein layers
Publikationen
Occurrence, location, ultrastructure and morphogenesis of S-layers.
Autoren: Sleytr, U. B., Messner, P., Pum, D., Sara, M. Jahr: 1996
Chapter in collected volumes
Anisotropic crystal growth of the S-layer of Bacillus sphaericus CCM 2177 at the air/water interface.
Autoren: Pum, D., Sleytr, U. B. Jahr: 1995
Journal articles
Two-dimensional protein crystals (S-layers): Fundamentals and applications for biomimetic membranes and molecular nanotechnology.
Autoren: Sleytr, U B; Pum, D; Sara, M Jahr: 1995
Journal articles
Molecular nanotechnology and biomimetics with S-layers.
Autoren: Pum, D., Sleytr, U. B. Jahr: 1996
Chapter in collected volumes
Nanonatural lithography with 2-D protein surface layers.
Autoren: Pum, D., Sleytr, U. B. Jahr: 1995
Conference & Workshop proceedings, paper, abstract
Supramolecular engineering with S-layer membranes.
Autoren: Pum, D., Sleytr, U. B. Jahr: 1996
Conference & Workshop proceedings, paper, abstract
Crystalline bacterial cell surface proteins. Molecular Biology Intelligence Unit.
Autoren: Sleytr, U. B., Messner, P., Pum, D., Sara, M. (eds.) Jahr: 1996
Editorial
Applications of S-layers.
Autoren: Sleytr, UB; Bayley, H; Sára, M; Breitwieser, A; Küpcü, S; Mader, C; Weigert, S; Unger, FM; Messner, P; Jahn-Schmid, B; Schuster, B; Pum, D; Douglas, K; Clark, NA; Moore, JT; Winningham, TA; Levy, S; Frithsen, I; Pankovc, J; Beale, P; Gillis, HP; Choutov, DA; Martin, KP Jahr: 1997
Journal articles
Two-dimensional protein crystals: fundamentals and applications for biomimetic membranes and molecular nanotechnology.
Autoren: Sleytr, U. B., Sara, M., Pum, D., Messner, P., Küpcü, S. Jahr: 1995
Conference & Workshop proceedings, paper, abstract
S-layers as structural elements in nanotechnology and biomimetics.
Autoren: Pum, D., Sleytr, U. B. Jahr: 1998
Conference & Workshop proceedings, paper, abstract
S-layer supported lipid membranes.
Autoren: Schuster, B., Pum, D., Sleytr, U. B. Jahr: 1998
Conference & Workshop proceedings, paper, abstract
Fourier-transform infrared studies of bacterial S-layers reconstituted at lipid surfaces.
Autoren: Schalke, M., Wetzer, B., Friedmann, J., Pum, D., Sleytr, U. B., Lösche, M. Jahr: 1998
Conference & Workshop proceedings, paper, abstract
Molecular nanotechnology with 2D-protein crystals (S-layers) at surfaces and interfaces.
Autoren: Sleytr, U. B., Pum, D. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
Molecular nanotechnology and biomimetics with S-layers.
Autoren: Sleytr, U. B., Pum, D. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
S-layers as supporting structures for biomimetic membranes.
Autoren: Pum, D., Schuster, B., Wetzer, B., Sleytr, U. B. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
Comparative voltage clamp studies on plain and S-layer supported lipid membranes.
Autoren: Schuster, B., Pum, D., Sleytr, U. B. Jahr: 1996
Conference & Workshop proceedings, paper, abstract
S-Schichten als Trägerstrukturen in der molekularen Nanotechnologie und Biomimetik.
Autoren: Pum, D., Sleytr, U. B. Jahr: 1995
Conference & Workshop proceedings, paper, abstract
Molecular nanotechnology and biomimetic membranes with 2D protein crystals (S-layers).
Autoren: Sleytr, U. B., Pum, D. Jahr: 1995
Conference & Workshop proceedings, paper, abstract
Introduction of new concepts for nanonatural lithography and biomimetic membranes.
Autoren: Pum, D., Sleytr, U. B. Jahr: 1995
Conference & Workshop proceedings, paper, abstract
Biomimetic membranes and molecular nanotechnology with 2-D protein crystals (S-layers).
Autoren: Sleytr, U B; Pum, D Jahr: 1998
Journal articles
Using crystalline bacterial surface layers as patterning elements in molecular nanotechnology.
Autoren: Pum, D., Sleytr, U. B. Jahr: 1998
Chapter in collected volumes
S-layer stabilized solid support lipid bilayers.
Autoren: Wetzer, B; Pum, D; Sleytr, UB Jahr: 1997
Journal articles
S-layer as pattering structures and supporting layers for biomimetic membranes.
Autoren: Pum, D; Wetzer, B; Schuster, B; Sleytr, U B Jahr: 1997
Journal articles
Reciprocal influence between the protein and lipid components of a lipid-protein membrane model.
Autoren: Diederich, A., Sponer, C., Pum, D., Sleytr, U. B., Lösche, M. Jahr: 1996
Journal articles
Bacterial S-layer protein coupling to lipids: X-ray reflectivity and grazing incidence diffraction studies.
Autoren: Weygand, M., Wetzer, B., Pum, D., Sleytr, U. B., Cuvillier, N., Kjaer, K., Howes, P. B., Lösche, M. Jahr: 1999
Journal articles
Supramolecular structure and function of S-layers.
Autoren: Pum, D., Sara, M., Sleytr, U. B. Jahr: 1998
Conference & Workshop proceedings, paper, abstract
S-layers: fundamentals and application in molecular nanotechnology.
Autoren: Sleytr, U. B., Pum, D. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
S-Schichten als Trägerstrukturen für Langmuir Blodgett (LB)-Filme und für die Entwicklung biomimetischer Membranen.
Autoren: Sleytr, U. B., Pum, D. Jahr: 1998
Forschungsbericht (extern. Auftraggeber)
S-Schichten als Trägerstrukturen für Langmuir Blodgett (LB)-Filme und für die Entwicklung biomimetischer Membranen.
Autoren: Sleytr, U. B., Pum, D. Jahr: 1999
Forschungsbericht (extern. Auftraggeber)
Crystalline bacterial cell surface layers (S-layers): from supramolecular cell structure to biomimetics and nanotechnology.
Autoren: Sleytr, U. B., Messner, P., Pum, D., Sara, M. Jahr: 1999
Journal articles
Kristalline Zelloberflächen-Schichten prokaryotischer Organismen (S-Schichten): von der supramolekularen Zellstruktur zur Biomimetik und Nanotechnologie.
Autoren: Sleytr, U B; Messner, P; Pum, D; Sara, M Jahr: 1999
Journal articles
Characterization of S-layer-supported bilayer lipid membranes.
Autoren: Schuster, B., Pum, D., Sleytr, U. B. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
S-layer proteins as basic building blocks in a biomolecular construction kit.
Autoren: Pum, D., Neubauer, A., Györvary, E., Sara, M., Sleytr, U. B. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
Probing the stability of S-layer-supported planar lipid membranes.
Autoren: Schuster, B; Sleytr, UB; Diederich, A; Bähr, G; Winterhalter, M; Jahr: 1999
Journal articles
The effect of S-layer protein adsorption and crystallization on the collective motion of a planar lipid bilayer studied by dynamic light scattering.
Autoren: Hirn, R; Schuster, B; Sleytr, UB; Bayerl, TM Jahr: 1999
Journal articles
Coupling of protein sheet crystals (S-layers) to phospholipid monolayers.
Autoren: Weygand, M., Schalke, M., Howes, P. B., Kjaer, K., Friedmann, J., Wetzer, B., Pum, D., Sleytr, U. B., Lösche, M. Jahr: 2000
Journal articles
Characterization and application potential of two-dimensional protein crystals (S-layers).
Autoren: Sleytr, U.B. Jahr: 1999
Conference & Workshop proceedings, paper, abstract
Crystalline bacterial cell surface layers (S-layers): a versatile self-assembly system.
Autoren: Sleytr, U.B., Sara, M., Pum, D. Jahr: 2000
Chapter in collected volumes
S-layer proteins as basic building blocks in a biomolecular construction kit.
Autoren: Sleytr, U.B., Pum, D., Sara, M. Jahr: 2000
Conference & Workshop proceedings, paper, abstract
2D protein crystals (S-layers) as structural basis for a supramolecular construction kit.
Autoren: Sleytr, U.B., Pum, D., Sara, M. Jahr: 2000
Conference & Workshop proceedings, paper, abstract
Formation of S-layer protein monolayers on solid substrates.
Autoren: Györvary, E., Pum, D., Sleytr, U.B. Jahr: 2000
Conference & Workshop proceedings, paper, abstract
Biomimetic membranes based on 2D-protein crystals.
Autoren: Sleytr, U.B., Pum, D., Sára, M. Jahr: 2000
Conference & Workshop proceedings, paper, abstract
S-Schichten als Trägerstrukturen für Langmuir Blodgett (LB)-Filme und für die Entwicklung biomimetischer Membranen.
Autoren: Sleytr, U.B., Pum, D. Jahr: 2000
Forschungsbericht (extern. Auftraggeber)
Two-dimensional protein crystals as structural basis for a supramolecular construction kit.
Autoren: Sleytr, U.B., Pum, D., Györvary, E., Sára, M. Jahr: 2000
Conference & Workshop proceedings, paper, abstract
S-layer ultrafiltration membranes: a new support for stabilizing functionalized lipid membranes.
Autoren: Schuster, B., Pum, D., Sara, M., Braha, O., Bayley, H., Sleytr, U.B. Jahr: 2001
Journal articles
S-layer-supported lipid membranes in the investigation of membrane proteins
Autoren: Schuster, B. Jahr: 1997
Conference & Workshop proceedings, paper, abstract
Structural reorganization of phospholipid headgroups upon recrystallization of an S-layer lattice
Autoren: Weygand, M., Kjaer, K., Howes, P.B., Wetzer, B., Pum, D., Sleytr U.B., Lösche, M. Jahr: 2002
Journal articles
Recrystallization of S-layer proteins on planar lipid bilayers and the effect on functional molecules.
Autoren: Schuster, B. Jahr: 1996
Conference & Workshop proceedings, paper, abstract
The application of bacterial S-layers in molecular nanotechnology
Autoren: Pum, D; Sleytr, UB Jahr: 1999
Journal articles
Advances in S-layer nanotechnology and biomimetics
Autoren: Sleytr, UB; Pum, D; Sara, M Jahr: 1996
Journal articles
Mitarbeiter*Innen
Uwe B. Sleytr
Em.O.Univ.Prof. Dipl.-Ing. Dr. Uwe B. Sleytr
uwe.sleytr@boku.ac.at
Project Leader
01.11.1994 - 31.05.2000
Dietmar Pum
Ao.Univ.Prof.i.R. Dipl.-Ing. Dr.techn. Dietmar Pum
dietmar.pum@boku.ac.at
Project Staff
01.11.1994 - 31.05.2000
Bernhard Schuster
Ao.Univ.Prof. Dipl.-Ing. Dr.techn. Bernhard Schuster
bernhard.schuster@boku.ac.at
Tel: +43 1 47654-80436
Project Staff
01.11.1994 - 31.05.2000
BOKU Partner
Externe Partner
Max Planck-Institut für Polymerforschung
Dr. W. Knoll
partner
University of Leipzig, Faculty of Physics and Earth Sciences
Prof. Dr. Matthias Lösche
partner