GEN-AU FUSARIUM: Molecular mechanisms of Fusarium virulence and host plant resistance

Adam Gerhard

Institute of Microbial Genetics

01.01.2003 - 31.05.2006

Research project (§ 26 & § 27)

Program GEN-AU - Genomforschung in Österreich

Funder Federal Ministries

Competencies

Abstract

GEN-AU pilot project The Fusarium graminearum fungus attacks grains and corn (maize), producing substances that are poisonous, have hormonal effects (for example, the mycotoxins deoxynivalenol and zearalenon), and can be hazardous to health. Which gene is it that enables the fungus to attack such an unusually large number of host plants? What mechanisms underlie the great resistance of some types of grains? The goal of this project is to find the genes that determine the virulence of the fungus and the chromosomes in grains that are responsible for greater resistance. This could, on the one hand, reveal new ways to combat the fungus, and on the other hand, lead to cultivation of highly resistant plant varieties.

Publications

Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana.

Autoren: Poppenberger, B; Berthiller, F; Lucyshyn, D; Sieberer, T; Schuhmacher, R; Krska, R; Kuchler, K; Glössl, J; Luschnig, C; Adam, G Jahr: 2003

Journal articles
Molecular analysis of the mycotoxin Zearalenone effects on the model plant Arabidopsis thaliana.

Autoren: Werner, U, Adam, G, Hauser, M-T Jahr: 2003

Conference & Workshop proceedings, paper, abstract
Glucosyltransferases from Arabidopsis thaliana inactivating the Fusarium toxins deoxynivalenol and zearalenone.

Autoren: Poppenberger, B., Berthiller, F., Lucyshyn, D., Mitterbauer, R., Schweiger, W., Schuhmacher, R., Krka, R., Adam, G. Jahr: 2004

Conference & Workshop proceedings, paper, abstract
Fusarium Mykotoxine – Molekularbiologische Ansätze zum Verständnis der Rolle von Deoxynivalenol und Zearalenon in der Pflanze-Pathogen Interaktion

Autoren: Mitterbauer, R., Poppenberger, B., Bachmann, H., Peterbauer, C., Safaie, N., Adam, G. Jahr: 2003

Conference & Workshop proceedings, paper, abstract
Biosynthese und Metabolismus von Zearalenon

Autoren: Mitterbauer, R; Wondrasch, K; Peruci, M; Berthiller F; Schuhmacher R; Krska, R; Nagl-Neuhold, G; Lemmens, M; Trail, F; Adam, G Jahr: 2005

Journal articles
Identifizierung eines neuen Virulenzgens von Fusarium graminearum mit einer zentralen Rolle in der Biosynthese von Sekundärmetaboliten

Autoren: Peruci, M; Berthiller, F; Schuhmacher, R; Krska, R; Nagl Neuhold, G; Lemmens, M; Mitterbauer, R Adam, G Jahr: 2005

Journal articles
Masked mycotoxins: determination of a deoxynivalenol glucoside in artificially and naturally contaminated wheat by liquid chromatography-tandem mass spectrometry.

Autoren: Berthiller, F; Dall'Asta, C; Schuhmacher, R; Lemmens, M; Adam, G; Krska, R Jahr: 2005

Journal articles
Development of an Affymetrix Genechip microarray for Fusarium graminearum using the GEN-AU/MIPS genome database

Autoren: Gueldener, U., Trail, F., Xu, JR., Adam, G. , Kistler C.H. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Phosphopantetheinyltransferase is a new virulence factor of Fusarium graminearum with a role in post-translational activation of polyketide and non-ribosomal peptide synthases

Autoren: Peruci, M., Berthiller, F., Mitterbauer, R., Cziferszky, A., Schuhmacher, R., Lemmens, M., Krska, R., Adam, G. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Biosynthese und Metabolismus von Zearalenon

Autoren: Mitterbauer, R., Wondrasch, K., Peruci, M., Berthiller, F., Schuhmacher, R., Krska, R., Nagl-Neuhold, G., Lemmens, M., Trail, F., Adam, G. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Arabidopsis thaliana: a model to study the effect of the estrogenic mycotoxin zearalenone in plants

Autoren: Werner, U., Berthiller, F., Schuhmacher, R., Krska, R., Adam, G., Hauser, M-T Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Identification of wheat cDNAs conferring deoxynivalenol resistance in yeast

Autoren: Lucyshyn, D., Weindorfer, H., Lemmens, M., Adam, G. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Phosphopantetheinyltransferase required for post-translational activation of polyketide synthases and non-ribosomal peptide synthases is a virulence factor of Fusarium graminearum

Autoren: Peruci, M., Berthiller, F., Lemmens, M. Schuhmacher, R., Krska, R., Cziferszky, A., Mitterbauer, R., Adam, G. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Characterization of deoxynivalenol and zearalenone-inactivating Arabidopsis thaliana UDP-glucosyltransferases

Autoren: Schweiger, W., Poppenberger, B., Lucyshyn, D., Berthiller, F., Schuhmacher, R., Krska, R., Mitterbauer, R., Adam, G. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Characterization of a putative sulfotransferase in Fusarium graminearum

Autoren: Thonhauser, K. Jahr: 2005

Master / Diploma Thesis
Inactivation of the Fusarium toxin deoxynivalenol by glucosylation

Autoren: Poppenberger B., Berthiller, F., Schweiger, W., Mitterbauer, R., Schuhmacher, R., Krska, R., Lemmens, M., Buerstmayr, H., Adam, G. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Ribosomal resistance to trichothecene toxins

Autoren: Mitterbauer, R., Lucyshyn, D., Abolmaali, S., Busch, B., Poppenberger, B., Mousavi, A., Weindorfer, H., Bürstmayr, H., Steiner, B., Lemmens, M., Adam, G. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids.

Autoren: Poppenberger, B; Fujioka, S; Soeno, K; George, GL; Vaistij, FE; Hiranuma, S; Seto, H; Takatsuto, S; Adam, G; Yoshida, S; Bowles, D Jahr: 2005

Journal articles
(P0016) Characterization of the effect of the Fusarium mycotoxin zearalenone in Arabidopsis thaliana

Autoren: Werner, U., Berthiller, F., Schuhmacher, R., Krska, R., Adam, G., Hauser, M.T. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Phosphopantetheinyltransferase required for post-translational activation of polyketide synthases and non-ribosomal peptide synthases is a virulence factor of Fusarium graminearum

Autoren: Peruci, M., Berthiller, F., Lemmens, M., Schuhmacher, R., Krska, R., Cziferszky, A., Mitterbauer, R., Adam, G. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Zearalenone is a virulence factor of Fusarium graminearum on wheat and affects ethylene signaling and expression of genes involved in cell wall modification/reinforcement in Arabidopsis thaliana

Autoren: Werner, U., Berthiller, F., Lemmens, M., Trail, F., Peruci, M., Schuhmacher, R., Krska, R., Cziferszky, A., Hauser, M-T., Adam, G. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Phosphopantetheinyltransferase required for post-translational activation of polyketide synthases and non-ribosomal peptide synthases is a virulence factor of Fusarium graminearum

Autoren: Peruci, M., Berthiller, F., Lemmens, M., Schuhmacher, R., Krska, R., Cziferszky, A., Mitterbauer, R., Adam, G. Jahr: 2006

Chapter in collected volumes
PPT1 - a virulence factor of Fusarium graminearum - encodes phosphopantetheinyltransferase required for post-translational activation of polyketide synthases and non-ribosomal peptide synthases

Autoren: Peruci, M., Lemmens, M., Berthiller, F., Schuhmacher, R., Krska, R., Cziferszky, A., Adam, G. Jahr: 2006

Conference & Workshop proceedings, paper, abstract
Development of new tools for Fusarium graminearum: Cre/lox mediated marker excision and bar-coded insertion mutants

Autoren: Mitterbauer, R., Cziferszky, A., Wiesenberger, G., Gueldener, U., Brunner, K., Hellmer, H., Mach, R., Adam, G., 2006 Jahr: 2006

Conference & Workshop proceedings, paper, abstract
Reduced virulence of a glucose oxidase producing strain of Fusarium graminearum on wheat

Autoren: Brunner, K., Lemmens, M., Kaspar, D., Köllner, K., Adam, G., Mach, R.L. Jahr: 2006

Conference & Workshop proceedings, paper, abstract
Biosynthesis and metabolism of zearalenone (ZON)

Autoren: Mitterbauer, R., Peruci, P., Cziferszky, A., Berthiller, F., Adam, G. Jahr: 2006

Conference & Workshop proceedings, paper, abstract
Heterologous expression of Arabidopsis UDP-glucosyltransferases in Saccharomyces cerevisiae for production of zearalenone-4-O-glucoside.

Autoren: Poppenberger, B; Berthiller, F; Bachmann, H; Lucyshyn, D; Peterbauer, C; Mitterbauer, R; Schuhmacher, R; Krska, R; Glössl, J; Adam, G; Jahr: 2006

Journal articles
Virulenzmechanismen von Fusarium graminearum und Resistenzmechanismen in Pflanzen

Autoren: Lemmens, M., Berthiller, F., Peruci, M., Poppenberger, B., Lucyshyn, D., Mitterbauer, R., Schuhmacher, R., Werner, U., Hauser, M.T., Krska, R., Bürstmayr, H., Adam, G. Jahr: 2006

Conference & Workshop proceedings, paper, abstract
Study of the metabolism of Fusarium mycotoxins in plants with HPLC - tandem mass spectrometry

Autoren: Berthiller, F., Lemmens, M., Werner, U., Krska, R., Hauser, M.-T., Adam, G., Schuhmacher, R. Jahr: 2006

Conference & Workshop proceedings, paper, abstract
Inactivation of Fusarium graminearum defence suppressing toxins by UDP-glucosyltransferases of Arabidopsis thaliana

Autoren: Poppenberger, B., Berthiller, F., Werner, U., Lucyshyn, D., Schweiger, W., Mitterbauer, R., Schuhmacher, R., Krska, R., Lemmens, M., Bürstmayr, H., Hauser, M-T., Glössl, J., Adam, G. Jahr: 2006

Conference & Workshop proceedings, paper, abstract
Screening of Fusarium graminearum mutants for loss of zearalenone production using yeast bioassays.

Autoren: Safaie, N., Peruci, M., Bachmann, H., Mitterbauer, R., Trail, F., Adam, G. Jahr: 2003

Conference & Workshop proceedings, paper, abstract
Recombinant yeast strains as tools for detection of Fusarium toxins and for cloning of detoxification genes

Autoren: Mitterbauer, R., Poppenberger, B., Lucyshyn, D., Peterbauer, C., Bachmann, H,, Weindorfer, H., Ellersdorfer, G., Adam, G. Jahr: 2004

Conference & Workshop proceedings, paper, abstract
Characterization of Arabidopsis thaliana UDP-glucosyl-transferases inactivating the Fusarium mycotoxin deoxynivalenol

Autoren: Poppenberger, B., Berthiller, F., Lucyshyn, D., Schuhmacher, R., Krska, R., Adam, G. Jahr: 2004

Conference & Workshop proceedings, paper, abstract
Inactivation of the Fusarium mycotoxins deoxynivalenol and zearalenone by members of the Arabidopsis thaliana 73C glycosyltransferase family.

Autoren: Poppenberger, B., Lucyshyn, D., Berthiller, F., Adam, G. Jahr: 2004

Conference & Workshop proceedings, paper, abstract
Determination of masked mycotoxins using HPLC - Tandem Mass Spectrometry

Autoren: Berthiller, F., Schuhmacher, R., Poppenberger, B., Lucyshyn, D., Adam, G., Krska, R. Jahr: 2004

Conference & Workshop proceedings, paper, abstract
Identifizierung von DON- und ZON-Glucosyltransferase-Genen von Arabidopsis thaliana und deren heterologe Expression zur Herstellung von Mykotoxin-Glucosid Referenzsubstanzen.

Autoren: Adam, G., Berthiller, F., Poppenberger, B., Lucyshyn, D., Schuhmacher, R., Krska, R. Jahr: 2004

Conference & Workshop proceedings, paper, abstract
DON-glycosides: characterisation of synthesis products and screening for their occurrence in DON-inoculated wheat samples.

Autoren: Dall’Asta, C., Berthiller, F., Schuhmacher, R., Adam, G., Lemmens, M., Krska, R. Jahr: 2004

Conference & Workshop proceedings, paper, abstract
Simultaneous Determination of Type A- & B-trichothecenes and zearalenone in cereals by HPLC-MS/MS.

Autoren: Schuhmacher, R., Berthiller, F., Buttinger, G., Krska, R. Jahr: 2004

Conference & Workshop proceedings, paper, abstract
Simultaneous determination of type A- and B-trichothecenes along with zearalenone in cereals by high performance liquid chromatography – tandem mass spectrometry.

Autoren: Berthiller, F., Schuhmacher ,R., Buttinger, G., Krska, R. Jahr: 2004

Conference & Workshop proceedings, paper, abstract
Toward solving the mysteries of quantitative resistance of wheat to Fusarium graminearum, the causal agent of head blight.

Autoren: Trognitz, F., Valiahdi, M., Trognitz, B., Burg, A., Wilhelm, E., Jankowicz, J., Bürstmayr, H., Lemmens, M., Grahsl, A. Jahr: 2004

Conference & Workshop proceedings, paper, abstract
Biotransformation of selected mycotoxins in wheat: Screening for metabolites by LC/MS/MS.

Autoren: Schuhmacher, R., Berthiller, F., Lemmens, M., Adam, G., Krska, R. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Determination of DON-3-Glucoside in artificially and naturally contaminated wheat with LC-MS/MS.

Autoren: Berthiller, F., Dall’Asta, C., Schuhmacher, R., Lemmens, M., Adam, G., Krska, R. Jahr: 2005

Conference & Workshop proceedings, paper, abstract
Identifizierung neuer DON-Resistenzgene von Weizen durch heterologe Expression von cDNAs in Bäckerhefe

Autoren: Lucyshyn, D; Weindorfer, H; Wilhelm, E; Lemmens, M; Adam, G Jahr: 2006

Journal articles
Effekte von Zearalenon auf Arabidopsis thaliana

Autoren: Werner, U; Berthiller F; Sulyok, M; Krska, R; Hauser, M-T; Schuhmacher, R Jahr: 2006

Journal articles
Inactivation of Fusarium graminearum defense suppressing toxins by plant UDP-glucosyltransferases

Autoren: Poppenberger, B., Berthiller, F., Werner, U., Lucyshyn, D., Schweiger, W., Mitterbauer, R., Glössl, J., Schuhmacher, R., Krska, R., Lemmens, M., Bürstmayr, H., Hauser, M-T., Adam, G. Jahr: 2006

Conference & Workshop proceedings, paper, abstract
Screening for resistance to deoxynivalenol in wheat

Autoren: Lemmens, M., Scholz, U., Berthiller, F., Schuhmacher, R., Mesterhazy, A., Krska, R., Bürstmayr, H. Jahr: 2006

Conference & Workshop proceedings, paper, abstract
PPT1 encoding phosphopantetheinyltransferase is required for posttranslational activation of PKSs and NRPSs and necessary for Fusarium graminearum virulence on wheat

Autoren: Peruci, M., Berthiller, F., Lemmens, M., Schuhmacher, R., Krska, R., Mitterbauer, R., Adam, G. Jahr: 2006

Conference & Workshop proceedings, paper, abstract
An European contribution to the genomics of Fusarium graminearum

Autoren: Adam, G., Güldener, U., Berthiller, F., Peruci, M., Mitterbauer, R., Werner, U., Hauser, M-T, Lemmens, M., Bürstmayr, H., Brunner, K., Mach, R.L., Krska, R. Jahr: 2006

Conference & Workshop proceedings, paper, abstract
Marker rescue via the Cre/lox system in Fusarium graminearum

Autoren: Mitterbauer, R., Czifersky, A., Wiesenberger, G., Güldener, U., Brunner, K., hellmer, H., Mach, R.L., Adam, G. Jahr: 2006

Conference & Workshop proceedings, paper, abstract
Determination of masked mycotoxins using HPLC-tandem mass spectrometry

Autoren: Berthiller, F., Schuhmacher, R., Poppenberger, B., Lucyshyn, D., Lemmens, M., Adam, G., Krska, R. Jahr: 2006

Chapter in collected volumes
Bildung von maskierten Fusarium Mykotoxinen in Pflanzen

Autoren: Berthiller, F; Werner, U; Adam, G; Krska, R; Lemmens, M; Sulyok, M; Hauser, M T; Schuhmacher, R Jahr: 2006

Journal articles
Detection and structural characterization of extracellular siderophores produced by Fusarium graminearum

Autoren: Berthiller, F., Adam, G., Peruci, M., Fog Nielsen, K., Krska, R., Schuhmacher, R. Jahr: 2007

Conference & Workshop proceedings, paper, abstract
Wheat cdnas conferring deoxynivalenol resistance in yeast: Ubiquitin availability and proteasome dependent protein degradation limit trichothecene resistance

Autoren: Lucyshyn, D., Abolmaali, S., Weindorfer, H., Lemmens, M., Adam, G. Jahr: 2007

Conference & Workshop proceedings, paper, abstract
The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization.

Autoren: Cuomo, C.A., Güldener, U., Xu, J.R., Trail, F., Turgeon, B.G., Di Pietro, A., Walton, J.D., Ma, L.J,. Baker, S.E., Rep, M., Adam, G., Antoniw, J., Baldwin, T., Calvo, S., Chang, Y.L., Decaprio, D., Gale, L.R,. Gnerre, S., Goswami, R.S., Hammond-Kosack, K., Harris, L.J., Hilburn, K., Kennell, J.C., Kroken, S., Magnuson, J.K., Mannhaupt, G., Mauceli, E., Mewes, H.W., Mitterbauer, R., Muehlbauer, G., Münsterkötter, M., Nelson, D., O'donnell, K., Ouellet, T., Qi, W., Quesneville, H., Roncero, M.I., Seong, K.Y., Tetko, I.V., Urban, M., Waalwijk, C., Ward, T.J., Yao, J., Birren, B.W., Kistler, H.C. Jahr: 2007

Journal articles
Towards the establishment of ZON reporters in plants

Autoren: Merz D, Werner U, Berthiller F, Neubert S, Krska R, Adam G, Hauser M-T, Jahr: 2009

Conference & Workshop proceedings, paper, abstract
Arabidopsis reporter plants for in vivo detection of the mycotoxin zearalenone – preparations for a mutagenesis screen to detect mutants with altered signaling, uptake or metabolization

Autoren: Merz D, Ayad M, Pfaffenbichler N, Adam G, Hauser M-T Jahr: 2010

Conference & Workshop proceedings, paper, abstract
The Fusarium toxins beta-zearalenol and zearalenone inhibit Hsp90 ATPase activity and are inactivated by glucosylation and sulfatation.

Autoren: Torres Acosta JA, Berthiller F, Wiesenberger G, Mitterbauer R, Werner U, Merz D, Hauser M-T, Shams M, Krska R, Adam G Jahr: 2010

Conference & Workshop proceedings, paper, abstract
Short review: Metabolism of theFusarium mycotoxins deoxynivalenol and zearalenone in plants.

Autoren: Berthiller, F; Lemmens, M; Werner, U; Krska, R; Hauser, MT; Adam, G; Schuhmacher, R; Jahr: 2007

Journal articles

Project staff

Gerhard Adam

Ao.Univ.Prof. Dipl.-Ing. Dr.rer.nat. Gerhard Adam
gerhard.adam@boku.ac.at
Tel: +43 1 47654-94480

Project Leader

01.01.2003 - 31.05.2006

Marie-Theres Hauser

Ao.Univ.Prof. Dipl.-Ing. Dr.nat.techn. Marie-Theres Hauser
marie-theres.hauser@boku.ac.at
Tel: +43 1 47654-94240

Sub Projectleader

01.01.2003 - 31.05.2006

Rudolf Krska

Univ.Prof. Dipl.-Ing. Dr.techn. Rudolf Krska
rudolf.krska@boku.ac.at
Tel: +43 1 47654-97301, 97302

Sub Projectleader

01.01.2003 - 31.05.2006

Marc Lemmens

Ao.Univ.Prof. Dipl.-Ing. Dr.nat.techn. Marc Lemmens
marc.lemmens@boku.ac.at
Tel: +43 1 47654-97104, 97158

Sub Projectleader

01.01.2003 - 31.05.2006

Kurt Brunner

Dipl.-Ing. Dr. Kurt Brunner

Project Staff

01.01.2003 - 31.05.2006

Andreas Gallmetzer

Dr. Andreas Gallmetzer

Project Staff

01.01.2003 - 31.05.2006

Christian Gladysz

Project Staff

01.01.2003 - 31.05.2006

Doris Lucyshyn

Mag. Dr. Doris Lucyshyn
doris.lucyshyn@boku.ac.at

Project Staff

01.01.2003 - 31.05.2006

Brigitte Poppenberger

Dipl.-Ing. Brigitte Poppenberger

Project Staff

01.01.2003 - 31.05.2006

Atikarn Sangpetch

Project Staff

01.01.2003 - 31.05.2006

Joseph Strauss

Univ.Prof. Mag.rer.nat. Dr.rer.nat. Joseph Strauss
joseph.strauss@boku.ac.at
Tel: +43 1 47654-94420

Sub Projectleader

01.01.2003 - 31.05.2006

Rainer Schuhmacher

Univ.Prof. Dipl.-Chem. Dr.rer.nat. Rainer Schuhmacher
rainer.schuhmacher@boku.ac.at
Tel: +43 1 47654-97307

Project Staff

01.01.2003 - 31.05.2006

Ulrike Werner

Project Staff

01.01.2003 - 31.05.2006

BOKU partners

External partners

Saatzucht Donau

Dr. Julia Lafferty

partner

ARC Seibersdorf Research GmbH, Life Sciences

Doz. Eva Wilhelm Dr. Bodo Trognitz

partner

University of Technology in Vienna, Inst. of Chemical Engineering _x000D_

none

partner

GEN-AU FUSARIUM: Molecular mechanisms of Fusarium virulence and host plant resistance

Abstract

Publications

Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP-glucosyltransferase from Arabidopsis thaliana.

Molecular analysis of the mycotoxin Zearalenone effects on the model plant Arabidopsis thaliana.

Glucosyltransferases from Arabidopsis thaliana inactivating the Fusarium toxins deoxynivalenol and zearalenone.

Fusarium Mykotoxine – Molekularbiologische Ansätze zum Verständnis der Rolle von Deoxynivalenol und Zearalenon in der Pflanze-Pathogen Interaktion

Biosynthese und Metabolismus von Zearalenon

Identifizierung eines neuen Virulenzgens von Fusarium graminearum mit einer zentralen Rolle in der Biosynthese von Sekundärmetaboliten

Masked mycotoxins: determination of a deoxynivalenol glucoside in artificially and naturally contaminated wheat by liquid chromatography-tandem mass spectrometry.

Development of an Affymetrix Genechip microarray for Fusarium graminearum using the GEN-AU/MIPS genome database

Phosphopantetheinyltransferase is a new virulence factor of Fusarium graminearum with a role in post-translational activation of polyketide and non-ribosomal peptide synthases

Biosynthese und Metabolismus von Zearalenon

Arabidopsis thaliana: a model to study the effect of the estrogenic mycotoxin zearalenone in plants

Identification of wheat cDNAs conferring deoxynivalenol resistance in yeast

Phosphopantetheinyltransferase required for post-translational activation of polyketide synthases and non-ribosomal peptide synthases is a virulence factor of Fusarium graminearum

Characterization of deoxynivalenol and zearalenone-inactivating Arabidopsis thaliana UDP-glucosyltransferases

Characterization of a putative sulfotransferase in Fusarium graminearum

Inactivation of the Fusarium toxin deoxynivalenol by glucosylation

Ribosomal resistance to trichothecene toxins

The UGT73C5 of Arabidopsis thaliana glucosylates brassinosteroids.

(P0016) Characterization of the effect of the Fusarium mycotoxin zearalenone in Arabidopsis thaliana

Phosphopantetheinyltransferase required for post-translational activation of polyketide synthases and non-ribosomal peptide synthases is a virulence factor of Fusarium graminearum

Zearalenone is a virulence factor of Fusarium graminearum on wheat and affects ethylene signaling and expression of genes involved in cell wall modification/reinforcement in Arabidopsis thaliana

Phosphopantetheinyltransferase required for post-translational activation of polyketide synthases and non-ribosomal peptide synthases is a virulence factor of Fusarium graminearum

PPT1 - a virulence factor of Fusarium graminearum - encodes phosphopantetheinyltransferase required for post-translational activation of polyketide synthases and non-ribosomal peptide synthases

Development of new tools for Fusarium graminearum: Cre/lox mediated marker excision and bar-coded insertion mutants

Reduced virulence of a glucose oxidase producing strain of Fusarium graminearum on wheat

Biosynthesis and metabolism of zearalenone (ZON)

Heterologous expression of Arabidopsis UDP-glucosyltransferases in Saccharomyces cerevisiae for production of zearalenone-4-O-glucoside.

Virulenzmechanismen von Fusarium graminearum und Resistenzmechanismen in Pflanzen

Study of the metabolism of Fusarium mycotoxins in plants with HPLC - tandem mass spectrometry

Inactivation of Fusarium graminearum defence suppressing toxins by UDP-glucosyltransferases of Arabidopsis thaliana

Screening of Fusarium graminearum mutants for loss of zearalenone production using yeast bioassays.

Recombinant yeast strains as tools for detection of Fusarium toxins and for cloning of detoxification genes

Characterization of Arabidopsis thaliana UDP-glucosyl-transferases inactivating the Fusarium mycotoxin deoxynivalenol

Inactivation of the Fusarium mycotoxins deoxynivalenol and zearalenone by members of the Arabidopsis thaliana 73C glycosyltransferase family.

Determination of masked mycotoxins using HPLC - Tandem Mass Spectrometry

Identifizierung von DON- und ZON-Glucosyltransferase-Genen von Arabidopsis thaliana und deren heterologe Expression zur Herstellung von Mykotoxin-Glucosid Referenzsubstanzen.

DON-glycosides: characterisation of synthesis products and screening for their occurrence in DON-inoculated wheat samples.

Simultaneous Determination of Type A- & B-trichothecenes and zearalenone in cereals by HPLC-MS/MS.

Simultaneous determination of type A- and B-trichothecenes along with zearalenone in cereals by high performance liquid chromatography – tandem mass spectrometry.

Toward solving the mysteries of quantitative resistance of wheat to Fusarium graminearum, the causal agent of head blight.

Biotransformation of selected mycotoxins in wheat: Screening for metabolites by LC/MS/MS.

Determination of DON-3-Glucoside in artificially and naturally contaminated wheat with LC-MS/MS.

Identifizierung neuer DON-Resistenzgene von Weizen durch heterologe Expression von cDNAs in Bäckerhefe

Effekte von Zearalenon auf Arabidopsis thaliana

Inactivation of Fusarium graminearum defense suppressing toxins by plant UDP-glucosyltransferases

Screening for resistance to deoxynivalenol in wheat

PPT1 encoding phosphopantetheinyltransferase is required for posttranslational activation of PKSs and NRPSs and necessary for Fusarium graminearum virulence on wheat

An European contribution to the genomics of Fusarium graminearum

Marker rescue via the Cre/lox system in Fusarium graminearum

Determination of masked mycotoxins using HPLC-tandem mass spectrometry

Bildung von maskierten Fusarium Mykotoxinen in Pflanzen

Detection and structural characterization of extracellular siderophores produced by Fusarium graminearum

Wheat cdnas conferring deoxynivalenol resistance in yeast: Ubiquitin availability and proteasome dependent protein degradation limit trichothecene resistance

The Fusarium graminearum genome reveals a link between localized polymorphism and pathogen specialization.

Towards the establishment of ZON reporters in plants

Arabidopsis reporter plants for in vivo detection of the mycotoxin zearalenone – preparations for a mutagenesis screen to detect mutants with altered signaling, uptake or metabolization

The Fusarium toxins beta-zearalenol and zearalenone inhibit Hsp90 ATPase activity and are inactivated by glucosylation and sulfatation.

Short review: Metabolism of theFusarium mycotoxins deoxynivalenol and zearalenone in plants.

Project staff

BOKU partners

External partners

Saatzucht Donau

ARC Seibersdorf Research GmbH, Life Sciences

University of Technology in Vienna, Inst. of Chemical Engineering _x000D_