Identification and characterization of expressed genes involved in Fusarium head blight resistance of wheat
Abstract
Fusarium head blight (FHB, scab) is a fungal disease of wheat that occurs in many cereal growing regions of the world. Fungi of the group Fusarium spp. may attack cereal plants at all stages, but most critical is the infection of the ears. The main threat associated with the disease is the contamination of cereals with mycotoxins. The cultivation of resistant varieties would contribute significantly to the integrated control of this disease. However, most currently grown wheat cultivars are susceptible to the disease.The genetics of FHB resistance in genotypes tracing to the cultivar 'Sumai3' has been studied extensively in recent years. Despite that several chromosomal regions with quantitative effects on resistance (quantitative trait loci, QTL) were identified, the actual position, sequence and function of the resistance genes is yet unknown. With the proposed project we aim to identify expressed genes involved in the resistance reaction of wheat against Fusarium head blight to contribute to the functional clarification of the resistance reaction. Near isogenic lines differing in one or two QTL regions (Qfhs.ndsu-3BS and Qfhs.ifa-5A) will be challenged by inoculation with Fusarium graminearum. We will use the cDNA-AFLP method, a gel¿based transcript profiling system, to study differential gene expression and/or DNA polymorphisms in transcripts between near isogenic lines at several time points after Fusarium inoculation. Differentially expressed transcripts will be recovered, cloned, sequenced and further functionally characterized.
wheat resistance breeding gene expression Fusarium
Publikationen
Identification and Characterization of Expressed Genes involved in Fusarium Head Blight Resistance of Wheat
Autoren: Steiner, B., M. Lemmens and H. Buerstmayr Jahr: 2004
Conference & Workshop proceedings, paper, abstract
Differential gene expression of wheat genotypes after Fusarium graminearum inoculation analyzed through cDNA-AFLPs
Autoren: Steiner B., Kurz H., Lemmens M., Buerstmayr H. Jahr: 2007
Conference & Workshop proceedings, paper, abstract
Differential gene expression of wheat genotypes after Fusarium graminearum inoculation analyzed through cDNA-AFLPs
Autoren: Steiner B., Kurz H., Lemmens M., Buerstmayr H. Jahr: 2007
Conference & Workshop proceedings, paper, abstract
Differential gene expression of related wheat lines with contrasting levels of head blight resistance after Fusarium graminearum inoculation.
Autoren: Steiner, B; Kurz, H; Lemmens, M; Buerstmayr, H Jahr: 2009
Journal articles
Gene expression analysis of related wheat lines with contrasting levels of head blight resistance after Fusarium graminearum inoculation
Autoren: Steiner, B; Schieszl, K; Litwicka, EP; Kurz, H; Lemmens, M; Jia, H; Muehlbauer, G; Buerstmayr, H Jahr: 2008
Journal articles
Gene expression analysis of related wheat lines with contrasting levels of head blight resistance after Fusarium graminearum inoculation
Autoren: Steiner Barbara, Apinun Limmongkon, Katharina Schiessl, Marc Lemmens, Hayan Jia, Gary Muehlbauer, Alexandra Posekany, David P. Kreil and Hermann Buerstmayr Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Gene expression analysis of related wheat lines with contrasting levels of head blight resistance after Fusarium graminearum inoculation
Autoren: Steiner Barbara, Apinun Limmongkon, Katharina Schiessl, Marc Lemmens, Hayan Jia, Gary Muehlbauer, Alexandra Posekany, David P. Kreil, Hermann Buerstmayr Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Mitarbeiter*Innen
Hermann Bürstmayr
Univ.Prof. Dipl.-Ing. Dr.nat.techn. Hermann Bürstmayr
hermann.buerstmayr@boku.ac.at
Tel: +43 1 47654-97101, 97102
Project Leader
01.12.2003 - 31.12.2008
Barbara Steiner
Dipl.-Ing. Dr. Barbara Steiner
barbara.steiner@boku.ac.at
Tel: +43 1 47654-97105
Sub Projectleader
01.12.2003 - 31.12.2008