Protein purification and structure analysis
Abstract
The human peroxidases include myeloperoxidase (MPO), eosinophil peroxidase (EPO). MPO and EPO play a central role in the antimicrobial defence system. MPO and EPO have also been implicated in promoting tissue damage in numerous inflammatory diseases. Despite the physiological relevance and molecular and enzymatic peculiarities of human peroxidases, a comprehensive study of the reactivity of redox intermediates involved in catalysis is still missing. The aims of this project are the purification of large amounts of MPO and EPO from human blood. The group has expertise in rapid kinetics (multi-mixing stopped-flow analysis) and in CD (circular dichroism) spectropolarimetry used in structural analysis.
keywords protein purification human peroxidases metalloproteins structure analysis
Publikationen
Project staff
Paul Georg Furtmüller
Ao.Univ.Prof. Dipl.-Ing. Dr.nat.techn. Paul Georg Furtmüller
paul.furtmueller@boku.ac.at
Tel: +43 1 47654-77277
Project Leader
28.07.1998 - 31.12.2030
Christa Jakopitsch
Mag. Dr. Christa Jakopitsch
christa.jakopitsch@boku.ac.at
Tel: +43 1 47654-33061
Project Staff
28.07.1998 - 31.12.2030
Christian Obinger
Univ.Prof. Mag. Dr.rer.nat. Christian Obinger
christian.obinger@boku.ac.at
Tel: +43 1 47654-10011, 77273
Project Staff
28.07.1998 - 31.12.2030
BOKU partners
External partners
Planta Natural Products
Dr. Andreas Kubin
partner