Engineering phase II detoxification of mycotoxins in yeast
Abstract
Plant pathogenic fungi of the genus Fusarium cause diseases of important crop plants (e.g. wheat, barley, rye, oat, maize). In addition to the yield reductions, the high economical importance of Fusarium diseases is due to the toxic fungal metabolites accumulating in the grain. After toxicological evaluation and intake studies, EU wide regulatory measures are in preparation for Fusarium mycotoxins. The protein biosynthesis inhibitor deoxynivalenol (DON) and the estrogenic metabolite zearalenone (ZON) are the most prevalent mycotoxins in Europe, and pose a potential threat to human and animal health. Detoxification mechanisms based on formation of mycotoxin-conjugates such as glucuronides and sulfates exist in plant and animal cells, and most likely also in the toxin producing fungus. In this project mycotoxin inactivating glucuronosyltransferases (UGTs) and sulfotransferases (SULTs) should be identified, and the conjugation products structurally characterized. The goal of this project is to genetically engineer baker’s yeast so that it can functionally express UGTs and SULT genes.
Publikationen
Hydrolysis of deoxynivalenol-3-O-glucoside
Autoren: Berthiller, F., Domig, K.J., Kneifel, W., Juge, N., Knasmüller, S., Adam, G., Krska, R., Schuhmacher, R. Jahr: 2007
Conference & Workshop proceedings, paper, abstract
Identification of a bacterial metabolite protecting the yeast Saccharomyces cerevisiae against DON toxicity
Autoren: Weindorfer, H., Ellersdorfer, G., Sessitsch, A., Safaie, N., Gorfer, M., Strauss, J., Bogad, I., Kneifel, W., Lemmens, M., Potthast, A., Adam, G. Jahr: 2007
Conference & Workshop proceedings, paper, abstract
Production of the masked mycotoxins zearalenone-4-glucoside, '&alpha'-zeraralenol-4-glucoside, '&beta'-zearalenol-4-glucoside
Autoren: Krenn, P., Berthiller, F., Schweiger, W., Hametner, C., Ludwig, R., Adam, G., Krska, R., Schuhmacher, R. Jahr: 2007
Conference & Workshop proceedings, paper, abstract
Production of the masked mycotoxins zearalenone-4-glucoside, '&alpha'-zeraralenol-4-glucoside, '&beta'-zearalenol-4-glucoside
Autoren: Krenn, P; Berthiller, F; Schweiger, W; Hametner, C; Ludwig, R; Adam, G; Krska, R; Schuhmacher, R Jahr: 2007
Journal articles
Preparation and characterization of the masked Fusarium mycotoxins zearalenone-4O-beta-D-glucopyranoside, alpha-zearalenol-4O-beta-D-glucopyranoside and beta-zearalenol-4O-beta-D-glucopyranoside by MS/MS and 2D-NMR
Autoren: Berthiller, F., Hametner, C., Krenn, P., Schweiger, W., Ludwig, R., Adam, G., Krska, R., Schuhmacher, R. Jahr: 2009
Journal articles
Direct quantification of deoxynivalenol glucuronide in human urine as biomarker of exposure to the Fusarium mycotoxin deoxynivalenol
Autoren: Warth, B; Sulyok, M; Berthiller, F; Schuhmacher, R; Fruhmann, P; Hametner, C; Adam, G; Frohlich, J; Krska, R Jahr: 2011
Journal articles
Project staff
Gerhard Adam
Ao.Univ.Prof. Dipl.-Ing. Dr.rer.nat. Gerhard Adam
gerhard.adam@boku.ac.at
Tel: +43 1 47654-94480
Project Leader
01.02.2006 - 31.01.2009