S-layer lipid membranes for membrane protein reconstitution (P20256-B11)
Abstract
Genome sequencing projects have revealed that membrane proteins represent about a third of the gene products in most organisms. Transmembrane (TM) proteins and membrane-associated proteins are targeted in many (infectious) diseases and thus, they are a preferred target for pharmaceuticals (currently more than 60% of all consumed drugs). Due to this important function, membrane models play an important part in unravelling the fundamental cellular processes involved and in screening for pathogens or drug candidates. In the present project lipid membranes anchored to a solid support which has previously been covered by a crystalline bacterial cell surface layer (S-layer) will be fabricated without the need of an aperture. These S-layer supported lipid membranes mimic the cell envelope structure of Archaea which dwell under very harsh conditions. Spherical, disc- and cylinder-shaped assemblies will be bound electrostatically or via specific interactions to many or few defined positions, respectively, on the S-layer lattice to form planar lipid membranes. The function of the S-layer lattice is manifold: stabilizing scaffoldings for the phospholipid bilayer and tetraetherlipid monolayer (and mixtures of the lipids), anchoring layer, spacer towards the solid support, ion reservoir, and as second layer on the top of the S-layer supported lipid membrane acting as an antifouling and nanoporous protecting layer. These S-layer supported lipid membranes differing in generation and anchoring strategy will be extensively characterized in terms of long-term stability and fluidity. Due to its planarity, a broad arsenal of techniques can be used to probe the structural and dynamic properties of these supported lipid membranes. The fluidity will be investigated by fluorescence microscopical and spectroscopical techniques. By applying the S-layer technology it is expected that the long-term robustness of fluid lipid membranes can be substantially increased. The final goal, however, is the reconstitution of functional skeletal muscle ryanodine receptor Ca2+ release channels, nicotinic acetylcholine receptors, and -hemolysin. These TM proteins have been chosen because of their clinical importance, availability of ligands, agonists and antagonists, antibodies, representing different membrane-spanning structures, and the accumulated knowledge in the literature. Single channel recordings are envisaged on all of these TM proteins reconstituted in S-layer supported lipid membranes. This project will develop systems where TM proteins can be studied under controlled conditions not only for basic research but also in screening for ligands, pathogens or drug candidates, agonists and antagonists, and the development of TM protein-based biosensors.
bacterial S-layer proteins supported lipid membranes stability and fluidity transmembrane proteins nanobiotechnology
Publikationen
Fabrication and characterization of functionalized S-layer supported lipid membranes
Autoren: Schuster, B., Sleytr, U.B. Jahr: 2008
Chapter in collected volumes
Surfaces functionalized with S layer lipid membranes for biosensor application
Autoren: Schuster, B., Kiene, E., Kempinger, S., Sleytr U.B. Jahr: 2008
Conference & Workshop proceedings, paper, abstract
S-Layer Stabilized Lipid Membranes: Reconstitution Matrix for Transmembrane Proteins
Autoren: Schuster, B., Kempinger, S., Kiene, E., Sleytr U.B. Jahr: 2008
Conference & Workshop proceedings, paper, abstract
S-LAYER – LIPID MEMBRANE AS FUNCTIONAL BIOMIMETIC MODEL MEMBRANE
Autoren: Schuster, B. Jahr: 2009
Conference & Workshop proceedings, paper, abstract
S-layer stabilized lipid membrane: a biomimetic model system
Autoren: Schuster, B. Jahr: 2009
Conference & Workshop proceedings, paper, abstract
Intact lipid vesicles reversibly tethered to a bacterial S-layer protein lattice
Autoren: Kepplinger, C; Ilk, N; Sleytr, UB; Schuster, B Jahr: 2009
Journal articles
Solid supported lipid membranes: New concepts for the biomimetic functionalization of solid surfaces
Autoren: Knoll, W; Naumann, R; Friedrich, M; Robertson, JWF; Losche, M; Heinrich, F; McGillivray, DJ; Schuster, B; Gufler, PC; Pum, D; Sleytr, UB Jahr: 2008
Journal articles
Surfaces functionalized with self-assembling S-layer fusion proteins for nanobiotechnological applications
Autoren: Ilk, N; Egelseer, EM; Ferner-Ortner, J; Kupcu, S; Pum, D; Schuster, B; Sleytr, UB Jahr: 2008
Journal articles
S-layer stabilized lipid membranes generated by vesicle fusion
Autoren: Schrems, A.J., Kiene, E., Sleytr, U.B., Schuster, B. Jahr: 2009
Conference & Workshop proceedings, paper, abstract
Switching the orientation of S-layer lattices on planar and spherical lipid structures
Autoren: Kiene, E., Küpcü, S., Schrems, A.J., Mayer, H., Pum, D., Sleytr, U.B., Schuster, B. Jahr: 2009
Conference & Workshop proceedings, paper, abstract
S-layer templated bioinspired synthesis of silica
Autoren: Gobel, C; Schuster, B; Baurecht, D; Sleytr, UB; Pum, D Jahr: 2010
Journal articles
Nanocarrier systems with S-layer proteins
Autoren: Ücisik, M.H., Küpcü, S., Sleytr, U.B., Schuster, B. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Tailored S-layer Surfaces for Lipid Bilayer Generation
Autoren: Schrems, A., Sleytr, U.B., Schuster, B. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Lipid membranes on S-layer lattices as biomimetic model system
Autoren: Schuster, B., Schrems, A., Kiene, E., Sleytr, U.B. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Nanobiotechnological Applications of S-Layer Proteins
Autoren: Sleytr, U.B.,Schuster, B. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Rapid solvent exchange method on chemically modified S-layer surface for lipid bilayer generation
Autoren: Schrems, A., Keine, E.,Küpcü, S., Sleytr, U.B., Schuster, B. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Generation of a positively charged lipid bilayer by the rapid solvent exchange method on S-layer coated substrates
Autoren: Schrems, A., Küpcü, S., Sleytr, U.B., Schuster, B. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Nanocarrier systems with S-layer proteins
Autoren: Ücisik, M.H., Küpcü, S., Sleytr, U.B., Schuster, B. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Switching the orientation of S-layer lattices on lipid membranes
Autoren: Kiene, E., Küpcü, S., Pum, D., Sleytr, U.B., Schuster, B. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Nanocarrier systems with S-layer proteins: S-layer coated emulsomes
Autoren: Ücisik, M.H., Küpcü, S., Sleytr, U.B., Schuster, B. Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Nanocarrier systems with S-layer proteins
Autoren: Ücisik, M.H., Küpcü, S., Sleytr, U.B., Schuster, B. Nanocarrier systems with S-layer proteins. Summer School on Nanoscale Science of Biological Interfaces. The International Center for Materials Research (ICMR) June 19 - July 1, 2010, . Jahr: 2010
Conference & Workshop proceedings, paper, abstract
Nanobiotechnological Applications of S-Layers
Autoren: Sleytr, U.B., Egelseer, E.M., Ilk, N., Messner, P., Schäffer, C., Pum, D., Schuster, B. Jahr: 2010
Chapter in collected volumes
S-Layers, Microbial, Biotechnological Applications
Autoren: Egelseer, E.M., Ilk, N., Pum, D., Messner, P., Schäffer, C., Schuster, B., Sleytr, U.B. Jahr: 2010
Chapter in collected volumes
Biomimetic S-layer stabilized lipid membranes
Autoren: Schuster, B., Kepplinger, C., Sleytr, U.B. Jahr: 2010
Chapter in collected volumes
S-layer lipid membranes for membrane protein reconstitution
Autoren: Schuster, B. Jahr: 2009
Forschungsbericht (extern. Auftraggeber)
S-layer lipid membranes for membrane protein reconstitution
Autoren: Schuster, B. Jahr: 2010
Forschungsbericht (extern. Auftraggeber)
Bilayer lipid membrane formation on a chemically modified S-layer lattice.
Autoren: Schrems, A; Kibrom, A; Küpcü, S; Kiene, E; Sleytr, UB; Schuster, B; Jahr: 2011
Journal articles
Biomimetic S layer protein stabilized lipid membranes
Autoren: Schuster, B., A. Schrems, M. Ücisik, S. Damiati, D. Pum, U.B. Sleytr. Jahr: 2011
Conference & Workshop proceedings, paper, abstract
Liposome fusion on proteinaceous S-layer lattices triggered via beta-diketone ligand-europium(III) complex formation
Autoren: Schrems, A; Larisch, VD; Stanetty, C; Dutter, K; Damiati, S; Sleytr, UB; Schuster, B Jahr: 2011
Journal articles
Multitechnique study on a recombinantly produced Bacillus halodurans laccase and an S-layer/laccase fusion protein
Autoren: Ferner-Ortner-Bleckmann, J; Schrems, A; Ilk, N; Egelseer, EM; Sleytr, UB; Schuster, B Jahr: 2011
Journal articles
Generation of S-layer supported functionalized lipid bilayers
Autoren: Schrems, A., Ces, O., Larisch, V.D., Dutter, K., Friedmann, J., Küpcü, S., Stanetty, C., Kibrom, A., Lohner, K., Sleytr, U.B., Schuster, B. Jahr: 2011
Journal articles
S-layer lipid membranes for membrane protein reconstitution
Autoren: Schuster, B. Jahr: 2011
Forschungsbericht (extern. Auftraggeber)
Characterization of the Ryanodine Receptor 1 by Patch-Clamp and on S-layer Supported Lipid Membranes
Autoren: Larisch, V.-D., Schrems, A., Sleytr, U.B., Hohenegger, M., Schuster, B. Jahr: 2011
Conference & Workshop proceedings, paper, abstract
Nanotechnology with S-Layer Proteins as Building Blocks
Autoren: Sleytr, U.B., Schuster, B., Egelseer, E.M., Pum, D., Horejs, C.M., Tscheliessnig, R., Ilk, N. Jahr: 2011
Chapter in collected volumes
Eine Membran auf Stelzen - Zetapotentialmessungen und die Medizin
Autoren: Schuster, B Jahr: 2011
Journal articles
S-layer lipid membranes for membrane protein reconstitution
Autoren: Schuster, B. Jahr: 2012
Forschungsbericht (extern. Auftraggeber)
Das Zetapotential informiert über die Proteinadsorption
Autoren: Luxbacher, T; Schuster, B Jahr: 2012
Journal articles
Generation of new functional nanocarrier systems: Emulsomes with an S-layer for specific IgG-targeting
Autoren: Ücisik, M.H., Küpcü, S., Sleytr, U.B., Schuster, B. Jahr: 2011
Conference & Workshop proceedings, paper, abstract
In Vitro Production of Voltage Dependent Anion Channel (VDAC) in Liposomes and Artificial Membrane
Autoren: Damiati, S., Zayni, S., Sleytr, U.B., Chopineau, J., Schuster, B., Sinner, E.K. Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Biomimetic S-layer stabilized lipid membranes as a platform to study antimicrobial peptides
Autoren: Schuster, B., Schrems, A.J., Damiati, S.A., Lohner, K., Hohenegger, M., Sleytr, U.B. Jahr: 2012
Conference & Workshop proceedings, paper, abstract
Nanotechnology with S-layer proteins
Autoren: Schuster, B., Sleytr, U.B. Jahr: 2013
Chapter in collected volumes
Epoxy-Acid Bi-functionalized Polyhedral Oligomeric Silsesquioxanes as Stamp Material for Nanocontact Printing Application
Autoren: Kastner, J., Lorrat, O., Rank, A., Dittert, B., Schuster, B., Schwarzinger, B., Mühlberger, M. Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Direct incorporation of cell-free (ex vivo) synthesized human membrane proteins into synthetic bioarchitecture
Autoren: Damiati, S., Zayni, S., Küpcü, S., Sleytr, U.B., Chopineau, J., Schuster, B., Sinner, E.K. Jahr: 2013
Conference & Workshop proceedings, paper, abstract
S-Layer Proteins
Autoren: Sleytr, U.B., Pum, D., Egelseer, E.M., Ilk, N., Schuster, B. Jahr: 2013
Chapter in collected volumes
Integrated Membrane Proteins for Synthetic Biosystems
Autoren: Damiati, S., Zayni, S., Sleytr, U.B., Chopineau, J., Schuster, B., Sinner, E.K. Jahr: 2013
Conference & Workshop proceedings, paper, abstract
Emulsomes modified with S-layer for lipophilic drug delivery
Autoren: Ücisik, M.H., Küpcü, S., Sleytr, U.B., Schuster, B. Jahr: 2013
Conference & Workshop proceedings, paper, abstract
S-layer proteins as basic building blocks for nanobiotechnological application
Autoren: Sleytr, U.B., Schuster, B., Pum, D. Jahr: 2014
Conference & Workshop proceedings, paper, abstract
S-layer lipid membranes for membrane protein reconstitution
Autoren: Schuster, B. Jahr: 2013
Forschungsbericht (extern. Auftraggeber)
Construction of An Artificial Membrane Platform for Synthetic Biology: Investigation of a Membrane Proteins in an S-layer Supported Lipid Membrane
Autoren: Damiati, S; Zayni, S; Schrems, A; Sleytr, UB; Chopineau, J; Schuster, B; Sinner, EK Jahr: 2014
Conference & Workshop proceedings, paper, abstract
Reconstitution of Ryanodine Receptor/Ca2+ Release Channels in S-Layer supported Lipid Membranes
Autoren: Larisch, V-D; Schrems, A; Sleytr, U.B; Hohenegger M; Schuster, B Jahr: 2014
Conference & Workshop proceedings, paper, abstract
Fabricating S-layer supported functionalized lipid bilayers
Autoren: Schrems, A; Larisch, V-D; Friedmann, J; Stanetty, C; Kibrom, A; Lohner, K; Sleytr, UB; Schuster, B Jahr: 2014
Conference & Workshop proceedings, paper, abstract
Characterizing in vitro membrane-assisted protein synthesis (iMAPS) and co-translational insertion into polymer membranes
Autoren: Zaba, C; Zafiu, C; Tan, DC-W; Zapf, T; Küpcü, S; Huber, C; Zayni, S; Damiati, S; Schuster, B; Sinner, EK Jahr: 2014
Conference & Workshop proceedings, paper, abstract
Graphene based Biosensor – a new approach for the study of small ligand binding kinetics in real-time
Autoren: Kotlowski, C; Mastrogiacomo, R; Pelosi, P; Schütz, S; Schuster, B; Schwaighofer, A; Kleber, C; Larisika, M; Nowak, C; Knoll, W Jahr: 2014
Conference & Workshop proceedings, paper, abstract
Biomimetic S-layer – lipid self-assemblies as platform for membrane-active peptides and proteins
Autoren: Schuster, B. Jahr: 2015
Conference & Workshop proceedings, paper, abstract
Bioarchitecture Design: from DNA to Functional Protein Reconstituted into Semi-Natural Environment
Autoren: Damiati, S., Zayni, S., Sleytr, U.B., Schuster, B., Sinner, E.-K. Jahr: 2015
Conference & Workshop proceedings, paper, abstract
Mitarbeiter*Innen
Bernhard Schuster
Ao.Univ.Prof. Dipl.-Ing. Dr.techn. Bernhard Schuster
bernhard.schuster@boku.ac.at
Tel: +43 1 47654-80436
Project Leader
15.02.2008 - 14.02.2013