Analysis of MIOX genes in syncytia
Abstract
Transcriptome analysis of syncytia induced by the cyst nematode Heterodera schachtii has shown that genes coding for myo-inositol-oxygenase (MIOX) are strongly expressed in syncytia. This enzyme is involved in a pathway that leads to the production of UDP-glucuronic acid but other possible functions have also been proposed. UDP-glucuronic acid is an important precursor for cell wall polysaccharides but can also be produced by a second pathway containing the enzyme UDP-glucose-dehydrogenase. In this project we will study the involvement of the MIOX pathway in the induction and maintenance of syncytia with the help of mutants and miRNA lines. The effect of downregulation of the MIOX pathway will be analysed with resistance tests, cell wall analysis (HPLC) and electron microscopy.
keywords Arabidopsis Heterodera schachtii Myo inositol Syncytium miRNA
Publikationen
Project staff
Holger Bohlmann
Dr. Holger Bohlmann
holger.bohlmann@boku.ac.at
BOKU Project Leader
01.03.2008 - 28.02.2011
BOKU partners
External partners
Universität Salzburg
Tenhaken, Raimund
partner