Direct electron transfer and electrode contacting of redox enzymes for the design of optimized biosensors and biofuel cells
Abstract
The possibility to realize implantable sensor-transmitter systems with a volume smaller than 1 mm3 and to measure physiologically relevant substances online, without distracting wiring of the organism, has great potential in basic science and medicine. Feasible power sources for these low power requiring systems are miniature biofuel cells with a long operating life-time and of much smaller size than batteries or capacitors. Biosensors and biofuel cells based on direct electron transfer (DET), which do not need intermediary relays – so called mediators – for electron transfer (ET) are of greatest interest in current studies. In both applications, efficient electronic communication between the enzymes and the electrode is of utmost importance to achieve low detection limits or high current densities in these miniaturized devices. To understand the principles for efficient DET, the focus is put on two model enzymes useful for biosensors or in biofuel cells: cellobiose dehydrogenase, CDH, catalyzing the anodic oxidation of various carbohydrates, and laccase, which reduces oxygen to water in the cathodic reaction or oxidizing catecholamins and phenolic analytes on the anode. Both enzymes have been shown to be capable of DET at acidic pH, but need to be improved by: (i) enhancing the catalytic turnover under physiological conditions, (ii) increased DET rates by optimization of ET pathways, and (iii) improved packing density, orientation and binding of the enzymes and operational stability of the electrode.
keywords electron transfer cellobiose dehydrogenase laccase biofuel cell biosensor
Publikationen
Membrane-Less Biofuel Cell Based on Cellobiose Dehydrogenase (Anode)/Laccase (Cathode) Wired via Specific Os-Redox Polymers
Autoren: Stoica, L., Dimcheva, N., Ackermann, Y., Karnicka, K., Guschin, D. A., Kulesza, P. J., Rogalski, J., Haltrich, D., Ludwig, R., Gorton, L., Schuhmann, W. Jahr: 2009
Journal articles
Cellobiose dehydrogenase from the ligninolytic basidiomycete Ceriporiopsis subvermispora.
Autoren: Harreither, W; Sygmund, C; Dünhofen, E; Vicuña, R; Haltrich, D; Ludwig, R; Jahr: 2009
Journal articles
Project staff
Roland Ludwig
Assoc. Prof. Dipl.-Ing. Dr. Roland Ludwig
roland.ludwig@boku.ac.at
Tel: +43 1 47654-75216
Project Leader
01.07.2009 - 30.06.2011
BOKU partners
External partners
Royal Institute of Technology, Department of Biochemistry and Biotechnology
none
partner
University Lund
none
partner