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Gewählte Master / Diploma Thesis:

David Wieser (2015): Mikrosatelliten-Entwicklung für die einjährige Schlammlingsart Cyperus fuscus.
Master / Diploma Thesis - Institut für Botanik (Botany), BOKU-Universität für Bodenkultur, pp 100. UB BOKU obvsg FullText

Data Source: ZID Abstracts
The annual wetland plant species Cyperus fuscus, as a representative for the semi-aquatic communities of dwarf rushes on periodically draining riverbanks, represents an ideal candidate for population genetic studies of natural and anthropogenic habitats and also serves to elucidate the role of the soil seed bank in the life cycle of the species itself. An overall of 21 microsatellite markers has been developed in this work making use of two different methods. 4 markers have been developed with the freeware programs MSATCOMMANDER (Faircloth, 2008) and primer3 (ROZEN & SKALETZKY, 2000) and 17 markers were created by ECOGENICS (Balgach, Switzerland). The markers were tested on a natural as well as on an anthropogenically severely affected population to identify both the impact of ecosystem disturbances on the genetic diversity and determine the role of the seed bank in the soil as a genetic memory. Likewise, the yet not exactly known ploidy of the species should be clearly stated. For this purpose the allelic richness, the heterozygosity, the inbreeding coefficient (FIS) as well as the fixation index (FST) between sub-populations of the soil seed bank and the overground sub-population have been determined. Both the allelic richness (3.23) and the heterozygosity (HOBS 0.134; HEXP 0.588) of the natural population compared to the allelic richness (2.62) and the heterozygosity (HOBS 0.059; HEXP 0.336) of the anthropogenic population show, that the "ecosystem dynamics" cause an increase in genetic diversity in natural populations. The Fixation index shows a significant differentiation between sub-populations of the anthropogenic population and no significant differentiation between the sub-populations of the natural population. Cyperus fuscus could be determined to be a diploid species. The 21 newly developed microsatellite markers will be useful in further research to analyse the population structure and the role of the soil seed bank in the life-cycle of this species.

Beurteilende(r): Bernhardt Karl Georg
1.Mitwirkender: Tremetsberger Karin

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