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Gewählte Master / Diploma Thesis:

Luzia Haider (2022): Beurteilung der Desinfektionswirkung von UV und ClO2 bei Trinkwasserproben mittels Durchflusszytometrie, ATP-Bestimmung und Kultivierung.
Master / Diploma Thesis - Institut für Siedlungswasserbau, Industriewasserwirtschaft und Gewässerschutz (SIG), BOKU-Universität für Bodenkultur, pp 128. UB BOKU obvsg FullText

Data Source: ZID Abstracts
Abstract:
The aim of this work was to determine and evaluate the disinfection effect in a drinking water supply system and in laboratory experiments with E. faecium. The UV disinfection and the disinfection by adding ClO2 were determined at the drinking water supply system with the aid of the culture method and flow cytometry (TCC and ICC). The stability of the water was examined on basis of the growth potential. In the laboratory experiments the disinfection effect was illustrated by disinfecting E. faecium with different concentrations of ClO2 using different methods (cultural, flow cytometry (SG1PI in DMSO, SG1PI in Tris, CFDA), and ATP method) and the methods were compared with one another. The results show that the disinfection effect could basically be shown with all methods. The disadvantage of the cultural method is that after the disinfection most of the cells could no longer grow. In the growth potential test, it could be shown that after UV disinfection alone, the drinking water is unstable after 10 days of storage at a temperature of 15 °C. It was also observed that with the dye CFDA in untreated E. faecium that were diluted in raw water, only 30 percent of the esterase activity could be determined. The comparison of all methods in the laboratory experiment shows that the results of the ICC when using DMSO compared to Tris buffer as a solvent for SG1PI fit better with the results of the ATP method and the esterase activity. The cultural method differs completely from the results of the other methods; the results of the ATP method and the CFDA staining match best. The results show that knowledge of the mechanism by which the cells are killed after disinfection is necessary for an adequate assessment of the disinfection effect. The appropriate detection method can then be selected, a combination of several methods being advantageous.

Beurteilende*r: Fürhacker Maria
1.Mitwirkender: Zunabovic-Pichler Marija

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