University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Gewählte Master / Diploma Thesis:
Hannes Mair
(2017):
Auftrennung und Quantifizierung von freien Aminosäuren und biogenen Aminen in Sojabohnen mittels UHPLC.
Master / Diploma Thesis - Institut für Lebensmittelwissenschaften,
BOKU-Universität für Bodenkultur,
pp 153.
UB BOKU
obvsg
- Abstract:
- Amino acids and biogenic amines play a crucial role in the animate organism. As basic building blocks of proteins, amino acids are essential during the protein biosynthesis. Biogenic amines, however, mostly come up in a negative context, given that in certain concentrations some of these agents cause intoxications and health afflictions. Nevertheless, polyamines (as components of living cells) gain in importance by now, because they are involved both in the proliferation of cells as well as the cell growth. Due to polyamines’ regulative effect on the maintenance of the regular cell function, their possible application in cancer therapy is currently under examination. During this master thesis, 100 soybean samples of various cultivars were tested for their concentration of amino acids and biogenic amines. For the analysis, an Ultra-High Performance Liquid Chromatography (UHPLC) - multicomponent method was chosen. In total, 23 amino acids and 14 biogenic animes were separated and analyzed. The analytes were derivatized with 6-Aminoquinolyl-N-hydroxysuccinimidyl (AQC) and afterwards, with the help of UV detection at a wavelength of 249 nm, quantitatively and qualitatively assessed. The selected analysis gradient made it possible to separate all 37 analytes within 9 minutes. The total cycle time of 15.6 min was attained through the subsequent cleaning of the column. In order to check and confirm the suitability of the chosen method, different validation parameters, such as linearity, precision, LOD/LOQ as well as recovery were determined. The measurement results of the 100 soybean samples exhibit distinct variations concerning the overall concentration of amino acids and biogenic amines. For amino acids, a minimum value of 1091,6 mg/kg soybean and a maximum value of 9936,6 mg/kg soybean have been detected. With regard to the concentration of biogenic amines, a minimum value of 121.7 mg/kg soybean, and a maximum value of 311. 8 mg/kg soybean have been detected.
- Beurteilende(r): Mayer Helmut
- 1.Mitwirkender: Schmidt Anatol