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Gewählte Master / Diploma Thesis:

Nicola Pacher (2019): DETECTION OF POTENTIAL CO-SELECTION OF ANTIBIOTIC AND DISINFECTANT RESISTANCE IN PERSISTENT L. MONOCYTOGENES.
Master / Diploma Thesis - Institut für Lebensmittelwissenschaften, BOKU-Universität für Bodenkultur, pp 63. UB BOKU obvsg

Data Source: ZID Abstracts
Abstract:
The food-borne bacterial pathogen Listeria (L.) monocytogenes and causative agent of Listeriosis poses a high risk for the young, old, pregnant and immunocompromised people, (YOPI). Next to Campylobacteriosis, Salmonellosis, Listeriosis represents the third most frequent cause of fatal food-borne disease with the highest hospitalization and case fatality rate among humans. Therefore sanitation and disinfection of food processing environments (FPE) including non-food contact surfaces and food contact surfaces is mandatory and of uppermost importance to ensure food quality and food safety. Nevertheless the widespread use of disinfectants may exert selective pressure for the development of microbial resistance mechanisms. Biocide resistance can be caused by strain mutation, amplification of endogenous chromosomal genes, and acquisition of resistance determinants in extrachromosomal genetic elements (e.g. plasmids or transposons). This tolerance to disinfectants and antibiotics may have been acquired from the microbiota present in a FPE, as strains harboring these tolerances may have enhanced fitness and persistence in manufacturing facilities. This study aimed to identify methods to find potential co-resistance of persistent L. monocytogenes isolates to different sanitizer/disinfectants and antibiotics, by determining their minimal inhibitory concentration (MIC) and the possible correlation of specific genetic markers for antibiotic and disinfectant resistance like the bcrABC gene cassette, the transposable element Tn6188 and the presence of plasmids and their potential correlation with those markers. This thesis was most of all an attempt and can be seen as a starting point in determination of co-resistances in L. monocytogenes, however finding answers to co-selection causes would have gone beyond the scope of this work.

Beurteilende(r): Domig Konrad

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