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Gewählte Master / Diploma Thesis:

Maria Schuller (2020): QPCR-BASED FOOD SAFETY CHARACTERIZATION OF CANNABIS SATIVA.
Master / Diploma Thesis - Institut für Lebensmittelwissenschaften, BOKU-Universität für Bodenkultur, pp 70. UB BOKU obvsg

Data Source: ZID Abstracts
Abstract:
Cannabis sativa is a very versatile plant and is getting to be more and more important as a food and food ingredient. As a result, food and drug authorities must have accurate methods to ensure the food safety and authenticity of Cannabis products. The objectiv of this study was to develop a fast and accurate system to differentiate potential drug-producing varieties of Cannabis sativa with high Δ-9-Tetrahydrocannabinol (THC) content from fiber hemp or food hemp strains with a THC content below 0,2 %. To define the chemotype of Cannabis sativa the Δ-9-Tetrahydrocannabinolacid (THCA) synthase gene was used to distinguish whether the BT- or the BD-allele is expressed. Depending on the chemotype, the genetic potential of a possible THC production can be predicted. By using single nucleotide polymorphism (SNP) differences in the THCA synthase gene, it could be shown that the first steps of a high-resolution melt analysis fulfill the expectations of distinguishing between the two chemotypes in different varieties of hemp. In addition, a primer/probe system turned out to be specific for a BT-allele verification using a FAM-MGB probe including three SNPs specific sequences for the BT-allele. The fast technique of a magnetic induction cycler was used to receive specific results.

Beurteilende(r): Domig Konrad

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