Advanced mass spectrometry strategies for metabolomics
Abstract
Despite the significant advantages that combining ion mobility mass spectrometry with all-ions QTOFMS, the highly complex nature of samples faced in metabolomics studies still poses great challenges for routine use of such workflows in metabolomics. One approach to address this limitation is the use of drift-time dependent quadrupole transmission profiles to facilitate a “bandpass” selection of precursor ions to be fragmented in the collision cell. In such a workflow, the transmission and/or collision energy applied in the high energy frame can be further directed by the drift separation (i.e. the quadrupole transmission is programmed to suit the drift times of relevant metabolites). This technology has enormous potential for metabolomics, but has not been tested or assessed with relevant compounds or real samples. In this project, we propose to investigate the use of continuous wide-band quadrupole isolation in combination with ion mobility separation to establish optimized acquisition settings and comprehensive datamining workflows with an outlook toward critical applications covering both identification and relative quantification in biological and environmental samples.
keywords Mass spectrometry Ion mobility Metabolomics
Publikationen
Project staff
Stephan Hann
Univ.Prof. Dr. Stephan Hann
stephan.hann@boku.ac.at
Tel: +43 1 47654-77001, 77191
Project Leader
05.12.2018 - 31.03.2022
Tim Causon
Assoc. Prof. PD Tim Causon B.Sc. Ph.D.
tim.causon@boku.ac.at
Tel: +43 1 47654-77101, 77187
Sub Projectleader
05.12.2018 - 31.03.2022
Max Lennart Feuerstein
Max Lennart Feuerstein MSc.
Tel: +43 1 47654-77166, 77172
Project Staff
05.12.2018 - 31.03.2022
BOKU partners
External partners
Agilent Technologies Inc.
none
partner