FFoQSI: Identification of heterotrophic bacteria in drinking water resources using MALDI-TOF-MS
Abstract
For water quality assessment the count of bacterial colonies on heterotrophic growth media is an im-portant parameter. For the identification and investigation of possible entry paths, taxonomic assign-ment of these colonies is important, but based on classical physiological approaches rather time consum-ing and laborious. MALDI-TOF-MS, as widely used in clinical areas, shows high potential for application in environmental microbiology, as it is capable of rapidly and reliably assigning taxonomy to bacterial colo-nies. This assignment is based on databases that are mainly focused on clinical bacterial strains, and for that reason the assignment of environmental isolates often fails. Therefore, a comprehensive database of MALDI-TOF-MS spectra from environmental water samples would be needed, to fasten taxonomic assignment for future bacterial isolates and ease the investigation of entry paths into water bodies. Within this project water samples from groundwater and riverbank filtrate along the Danube River will be gathered before and after disinfection on a monthly basis and cultivated on an unspecific heterotrophic growth media. The present bacterial isolates will further be identified using MALDI-TOF-MS. If an identi-fication with this method is not possible, molecular biological methods will be used to fill the gaps. This will help us to understand, which heterotrophic bacteria are present at different water resources at dif-ferent points in time, and how they are affected by different disinfection methods. Temporal and spatial distribution patterns of cultivated bacteria identified by MALDI-TOF-MS might be different to non-culturable bacteria which are only visible using methods like DNA-Sequencing. A comparison of these two methodological approaches might show how cultivation shifts the perception of the bacterial com-munities in environmental samples, and how representative heterotrophic plate counts are for the as-sessment of drinking water quality.
Publikationen
Project staff
Philipp Proksch
Dipl.-Ing. Philipp Proksch
philipp.proksch@boku.ac.at
Tel: +43 1 47654-81118, 81142
Project Leader
01.05.2022 - 31.12.2023
Johanna Burtscher
Ass.Prof. Dipl.-Ing. Dr. Johanna Burtscher
johanna.burtscher@boku.ac.at
Tel: +43 1 47654-75456
Project Staff
01.05.2022 - 31.12.2023
Konrad Domig
Univ.Prof. Dipl.-Ing. Dr.nat.techn. Konrad Domig
konrad.domig@boku.ac.at
Tel: +43 1 47654-75453
Project Staff
01.05.2022 - 31.12.2023
Aurora Gitto
Aurora Gitto MSc.
aurora.gitto@boku.ac.at
Tel: +43 1 47654-81141
Project Staff
01.05.2022 - 31.12.2023
Magdalena Schöller
Magdalena Schöller B.Sc.
magdalena.schoeller@boku.ac.at
Project Staff
01.05.2022 - 31.12.2023
Irina Tanuwidjaja
Irina Tanuwidjaja Ph.D.
irina.tanuwidjaja@boku.ac.at
Project Staff
01.05.2022 - 31.12.2023
BOKU partners
External partners
Linz Service GmbH
none
partner