Structure and mechanism of the flavocytochrome cellobiose dehydrogenase from different fungi

Haltrich Dietmar

Institute of Food Technology

01.01.2004 - 31.12.2005

Self-financed Projects

Program none

Funder none

Competencies

Biotechnologie

Abstract

It is the objectives of the proposed project to compare the properties of cellobiose dehydrogenase (CDH) from basidiomycetes and ascomycetes and to investigate basic catalytic properties, the primary structure, and the reaction mechanism of different CDH belonging to class-1 and class-2. Such a study has not been performed previously, will investigate and highlight differences in the catalytic potential of representative enzymes of each class, and will help us to understand basic reaction mechanisms of these flavocytochromes. A more detailed knowledge will be the basis for a wider biotechnological application of these enzymes, e.g., in bioremediation, biosensors, or biocatalysis.

Publications

Cellbiose dehydrogenase from the white rot fungus Ceriporiopsis subvermispora: Screening, fermentation and initial characterisation.

Autoren: Dünhofen, E, Ganster, C., Haltrich, D., Ludwig, R. Jahr: 2005

Conference & Workshop proceedings, paper, abstract

external links and characteristics of the publication:
Direct electron transfer--a favorite electron route for cellobiose dehydrogenase (CDH) from Trametes villosa. Comparison with CDH from Phanerochaete chrysosporium.

Autoren: Stoica, L; Ruzgas, T; Ludwig, R; Haltrich, D; Gorton, L Jahr: 2006

Journal articles

external links and characteristics of the publication:

dx.doi.org
Application of cellulose-based self-assembled tri-enzyme system in a pseudo-reagent-less biosensor for biogenic catecholamine detection

Autoren: Rabinovich, M L; Vasil'chenko, L G; Karapetyan, K N; Shumakovich, G P; Yershevich, O P; Ludwig, R; Haltrich, D; Hadar, Y; Kozlov, Y P; Yaropolov, A I Jahr: 2007

Journal articles

external links and characteristics of the publication:
Fermentation, purification and characterization of cellobiose dehydrogenase from Ceriporiopsis subvermispora

Autoren: Evelyn Dünhofen Jahr: 2005

Master / Diploma Thesis

external links and characteristics of the publication:
Characterisation of newly found Cellobiose Dehydrogenases from Ceriporiopsis subvermispora and Myriococcum thermophilum combining methods from biochemistry, molecular biology and bioinformatics

Autoren: Ganster Christina Nicole Jahr: 2005

Master / Diploma Thesis

external links and characteristics of the publication:
Cellobiose dehydrogenase from the white rot fungus Ceriporiopsis subvermispora: Detailed kinetic and phylogenetic characterisation.

Autoren: Ganster, C., Dünhofen, E., Haltrich, D., Ludwig, R. Jahr: 2005

Conference & Workshop proceedings, paper, abstract

external links and characteristics of the publication:
Cellobiose dehydrogenase--a flavocytochrome from wood-degrading, phytopathogenic and saprotropic fungi.

Autoren: Zamocky, M; Ludwig, R; Peterbauer, C; Hallberg, BM; Divne, C; Nicholls, P; Haltrich, D Jahr: 2006

Journal articles

external links and characteristics of the publication:

Project staff

Dietmar Haltrich

Univ.Prof. Dipl.-Ing.Dr.techn. Dietmar Haltrich
dietmar.haltrich@boku.ac.at
Tel: +43 1 47654-75211

Project Leader

01.01.2004 - 31.12.2005

Clemens Karl Peterbauer

Assoc. Prof. Dr. Clemens Karl Peterbauer
clemens.peterbauer@boku.ac.at
Tel: +43 1 47654-75212

Project Staff

01.01.2004 - 31.12.2005

BOKU partners

Institute of Food Technology

role coordinator

External partners

A.N.Bach Institute of Biochemistry, Academy of Sciences of Russia

Mikhail L. Rabinovich

partner

Structure and mechanism of the flavocytochrome cellobiose dehydrogenase from different fungi

Abstract

Publications

Cellbiose dehydrogenase from the white rot fungus Ceriporiopsis subvermispora: Screening, fermentation and initial characterisation.

Direct electron transfer--a favorite electron route for cellobiose dehydrogenase (CDH) from Trametes villosa. Comparison with CDH from Phanerochaete chrysosporium.

Application of cellulose-based self-assembled tri-enzyme system in a pseudo-reagent-less biosensor for biogenic catecholamine detection

Fermentation, purification and characterization of cellobiose dehydrogenase from Ceriporiopsis subvermispora

Characterisation of newly found Cellobiose Dehydrogenases from Ceriporiopsis subvermispora and Myriococcum thermophilum combining methods from biochemistry, molecular biology and bioinformatics

Cellobiose dehydrogenase from the white rot fungus Ceriporiopsis subvermispora: Detailed kinetic and phylogenetic characterisation.

Cellobiose dehydrogenase--a flavocytochrome from wood-degrading, phytopathogenic and saprotropic fungi.

Project staff

Dietmar Haltrich

Clemens Karl Peterbauer

BOKU partners

Institute of Food Technology

External partners

A.N.Bach Institute of Biochemistry, Academy of Sciences of Russia

Funder

Austrian Development Agency (ADA)

Eigenfinanzierung - Universität für Bodenkultur Wien (Bundesmittel)