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Gewählte Master / Diploma Thesis:

Susanne Mahnik (2002): Stoffflussanalysen ausgewählter Zytostatika im Wiener Allgemeinen Krankenhaus und Entwicklung einer Analysemethode zum quantitativen Nachweis von 5-Fluorouracil im Abwasser mittels Kapillarelektrophorese.
Master / Diploma Thesis, BOKU-Universität für Bodenkultur, pp 117. UB BOKU obvsg

Data Source: ZID Abstracts
Cytostatic agents, which are used for the treatment of malign tumors, can get into the waste water or into the environment by natural excreta. Because of their mode of operation that aims at destroying the cancer cells, many of the cytostatics have teratogenic, mutagenic and carcinogenic effects. The objective of the present thesis is to present a literature review with the latest developments regarding consumption, dosage, excretion and biodegradation of selected cytostatic agents and the development of a method to measure traces of a selected antineoplastic agent. Besides the consumption of selected cytostatics in a Viennese Hospital, in particular of the divisions of oncology, is presented and possible concentrations in the waste water of the Viennese Hospital are calculated. The selection of the antineoplastic agents was done by consumption and toxicological data. Regarding the consumption, the most important cytostatic agents in Austria are the substances 5-fluorouracil, gemcitabine and the alkylating cytostatics cyclophosphamide and ifosfamide. None of them, except 5-fluorouracile, are biodegradable. The developed chemical method serves the quantitative detection of 5-fluorouracil in waste water using the capillary electrophoresis. The waste water has to be concentrated by solid phase extraction on ENV+ (factor 500). The solid phase extraction and the composition of the buffer (80% 160 mM sodium-borate buffer/20% acetonitrile) represent 2 special aspects of this method. The range of the calibration curve (5 - 500 µg/l), which is based on the possible concentrations in the waste water of the hospital, results in a linearity of 0,999. Regarding the recovery, an average value of 80% was reached. The validation was done by following the instructions given in the Journal of Chromatography B. In the waste water of the Viennese hospital cumulative concentrations of 14 µg cytostatics per l are possible, which corresponds to the data that was found in the respective literature (15 - 160 µg/l). Further research on the presence of antineoplastic agents in the environment, especially a complete risk assessment about possible negative impacts on the environment is still missing.

Beurteilende(r): Fürhacker Maria

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