Gewählte Master / Diploma Thesis:
Eva Maria Dorninger
(1996):
Etablierung der Kapillarelektrophrese in der Qualitätskontrolle rekombinanter Proteine und Antikörper.
Master / Diploma Thesis,
BOKU-Universität für Bodenkultur.
UB BOKU
obvsg
Data Source: ZID Abstracts
- Abstract:
- In this work various techniques of capillary electrophoresis (capillary
zone electrophoresis, capillary isoelectric focusing) were eveluated to its
suitability in quality control of recombinant proteins and antibodies. CZE
was calibrated for quantification of recombinant human superoxide dismutase
and the human monoclonal antibody 3D6 (anti-HIV).
In another application CZE was applied to analyse samples from an
accelerated stability test of an alkaline antibody.
In isoelectric focusing good separation was obtained with the model
proteins Myoglobine and beta-Lactoglobuline A under neutral and acidic
conditions, but not under alkaline.
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Beurteilende(r):
Bayer Karl