University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Wagner, A; Vorauer-Uhl, K; Kreismayr, G; Katinger, H; .
(2002):
Enhanced protein loading into liposomes by the multiple crossflow injection technique.
J Liposome Res. 2002; 12(3):271-283
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- Abstract:
- Methods for encapsulation of a drug into liposomes should preferably result in a high encapsulation efficiency and a high encapsulation capacity. Our studies were focussed on the establishment of an efficient encapsulation procedure of the radical scavenging protein, rh-Cu/Zn-SOD, into liposomes with the cross flow injection method. Limitations to increase the encapsulation efficiency are caused by the, enclosed aqueous volume, by the lipid concentration, the aspired vesicle size and the final ethanol concentration. Our research was performed to maximize the encapsulation following several strategies of injecting higher lipid concentrations. into the aqueous phase. The one way triple technique, a sophisticated preparation procedure is presented, which enables three times higher encapsulation rates in comparison to standard procedures. Additionally, scalability studies demonstrate reproducibility independent of the preparation volume. Vesicle size distribution and encapsulation efficiency remain constant. Furthermore, special attention is paid on reproducibility of prepared liposomes, scale-up and on long term stability of the lipid vesicles.
- Authors BOKU Wien:
- Katinger Hermann
- Vorauer-Uhl Karola
- Find related publications in this database (using NML MeSH Indexing)
- 1,2-Dipalmitoylphosphatidylcholine/chemistry;Amines/chemistry;Buffers;Chemistry, Pharmaceutical;Cholesterol/chemistry;Copper/chemistry;Drug Carriers/chemistry;Drug Stability;Equipment Design;Escherichia coli/genetics;Humans;Liposomes/analysis;Liposomes/chemistry*;Recombinant Proteins/chemistry;Reproducibility of Results;Superoxide Dismutase/analysis;Superoxide Dismutase/chemistry*;Technology, Pharmaceutical;Zinc/chemistry;
- Find related publications in this database (Keywords)
- liposome production
- scalability
- reproducibility
- encapsulation efficiency
- high protein entrapment
- rh-Cu/Zn-SOD
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