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Selected Publication:

Nallani, M; Andreasson-Ochsner, M; Tan, CW; Sinner, EK; Wisantoso, Y; Geifman-Shochat, S; Hunziker, W.
(2011): Proteopolymersomes: in vitro production of a membrane protein in polymersome membranes.
Biointerphases. 2011; 6(4):153-157 FullText FullText_BOKU

Polymersomes are stable self-assembled architectures which mimic cell membranes. For characterization, membrane proteins can be incorporated into such bio-mimetic membranes by reconstitution methods, leading to so-called proteopolymersomes. In this work, we demonstrate the direct incorporation of a membrane protein into polymersome membranes by a cell-free expression system. Firstly, we demonstrate pore formation in the preformed polymersome membrane using a-hemolysin. Secondly, we use claudin-2, a protein involved in cell-cell interactions, to demonstrate the in vitro expression of a membrane protein into these polymersomes. Surface plasmon resonance (Biacore) binding studies with the claudin-2 proteopolymersomes and claudin-2 specific antibodies are performed to show the presence of the in vitro expressed protein in polymersome membranes. (C) 2011 American Vacuum Society. [DOI: 10.1116/1.3644384]
Authors BOKU Wien:
Ehmoser Eva-Kathrin
Tan Cherng-Wen Darren

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