University of Natural Resources and Life Sciences, Vienna (BOKU) - Research portal
Selected Publication:
Fern ndez-S nchez, C; Tzanov, T; Gübitz, GM; Cavaco-Paulo, A.
(2002):
Voltammetric monitoring of laccase-catalysed mediated reactions.
Bioelectrochemistry. 2002; 58(2):149-156
- Abstract:
- Six different compounds capable of mediating laccase-catalysed reactions have been tested by cyclic voltammetry. They exhibited quasi-reversible electrodic behaviour with formal redox potentials ranging from 150 to 800 mV (E-0t vs. SCE). The immersion of a laccase-coated glassy carbon electrode (GCE) in mediator solutions generated large cathodic catalytic currents easily recorded by cyclic voltammetry at low-potential scan rates. This current showed two well-defined pH profiles, which correlated with the variation of the mediator redox potentials at the pH range tested. The relevant effect of temperature on the activity of laccase has been assessed here. Likewise, it was shown that the cut-rent record varied with the substrate concentration. This trend fitted Michaelis-Menten kinetics, which allowed us to give an estimation of the affinity of the fungal laccase for the different mediators. (C) 2002 Elsevier Science B.V. All rights reserved.
- Authors BOKU Wien:
- Gübitz Georg
- Find related publications in this database (using NML MeSH Indexing)
- Basidiomycota - enzymology
- Catalysis -
- Electrochemistry -
- Electron Transport -
- Hydrogen-Ion Concentration -
- Kinetics -
- Laccase -
- Models, Chemical -
- Oxidation-Reduction -
- Oxidoreductases - chemistry
- Reducing Agents - chemistry
- Thermodynamics -
- Find related publications in this database (Keywords)
- laccase
- mediator
- cyclic voltammetry
- catalytic current
- immobilisation
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