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Selected Publication:

Oburger, E; Dellxxxmour, M; Hann, S; Wieshammer, G; Puschenreiter, M; Wenzel, WW.
(2013): Evaluation of a novel tool for sampling root exudates from soil-grown plants compared to conventional techniques
ENVIRON EXP BOT. 2013; 87: 235-247. FullText FullText_BOKU

Abstract:
The release of low molecular weight (LMW) organic compounds (e.g. organic acids, amino acids, sugars, etc.) by living plant roots significantly contributes to the development of chemical, physical as well as microbial rhizosphere gradients. Suitable and accurate sampling procedures are crucial for enhancing our understanding of the dynamics of related rhizosphere processes. Here we compare common sampling techniques with a novel tool for root exudate collection that allows non-destructive and repetitive sampling from soil-grown roots. Root exudates from Zea mays L were collected using the following techniques: (i) hydroponic growth and sampling, (ii) soil growth and hydroponic sampling and (iii) rhizoboxes fitted with a novel in situ root exudate collecting tool. Furthermore, rhizosphere soil solution for the analysis of exudates and microbial metabolites was sampled using micro-suction cups (iv). The effect of different sampling solutions (deionised water and 0.5 mM CaCl2) on organic acid and amino acid exudation patterns was also investigated. The novel exudate collecting tool was successfully tested for root exudate sampling. Results showed that particularly amino acid exudation rates were significantly affected by growth conditions and sampling procedures, while organic acid exudation patterns varied less across the different sampling setups. Despite qualitative and quantitative differences, exudation rates were in the same order of magnitude across the different sampling procedures. Soil solution concentrations obtained from micro-suction-cup sampling at defined distance to the root surface showed no distinct gradient, highlighting the importance of soil microorganisms in regulating the soil solution concentration of LMW C compounds either via microbial degradation or the release of microbial metabolites. The exudate collector offers new opportunities to assess root exudation rates and composition from soil-grown plants and thus enhances our knowledge of fundamental rhizosphere processes. (C) 2012 Elsevier B.V. All rights reserved.
Authors BOKU Wien:
Hann Stephan
Oburger Eva
Puschenreiter Markus
Wenzel Walter
Werneth Madeleine
BOKU Gendermonitor:


Find related publications in this database (Keywords)
Rhizobox
Root exudates
Hydroponic
Nutrient solution
Citrate
Malate
Oxalate
Malonate
Amino acids
Organic acids
Carboxylates


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