Assessment of the state of genetic diversity of steppe plants in Austrian dry habitats as a basis for the detection of future trends

The random fluctuation of allele frequencies (genetic drift) can have a significant impact on evolution in small and fragmented populations and lead to an extinction vortex, a feedback loop between reduced population size, loss of genetic diversity and inbreeding. Since dry meadows and pastures in Austria are among the most species-rich habitats, but also among the most endangered due to changes in land use, we selected 14 rare "steppe plants" with occurrences in the Pannonian and partly also in the Alpine region for the survey of the state of genetic diversity: Artemisia pancicii; Astragalus exscapus; Crambe tataria; Dianthus serotinus; Dracocephalum austriacum; Iris humilis subsp. arenaria; Adonis vernalis; Phlomoides tuberosa; Carex supina; Linum flavum; Onobrychis arenaria; Oxytropis pilosa; Stipa capillata; and Pulsatilla grandis, P. oenipontana and P. vulgaris (species group). For the molecular genetic characterization of the populations and for a corresponding (long-term) comparison of genetic diversity (heterozygosity, gene flow, inbreeding), the occurrences are to be genotyped using RADseq. We expect that the data will provide valuable information for assessing the conservation status of the species themselves, but also of the habitats in which they occur. The data can be valuable as a basis for conservation decisions. For example, it can be used to assess which protected areas are characterized by high or low genetic diversity, what connections exist between protected areas or what contribution species conservation projects make to the preservation of genetic diversity. The project supports Austria's efforts as a party to the Convention on Biological Diversity to establish a national system for monitoring the status and development of biodiversity and its components by collecting data for indicator S.3.1 (status of genetic diversity of wild species) throughout Austria using a standardized design.