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Gewählte Master / Diploma Thesis:

Christiane Mair (2006): Aufklärung von Antibiotikaresistenz-Phänomenen ausgewählter Bifidobacterium spp.-Stämme isoliert aus unterschiedlichen Bereichen der Lebensmittelkette.
Master / Diploma Thesis - Abteilung Lebensmittelmikrobiologie und –hygiene (LMH), BOKU-Universität für Bodenkultur, pp 165.

Data Source: ZID Abstracts
Abstract:
In total, a number of 175 strains of Bifidobacterium species, predominantly of animal origin, have been analysed with microbiological and molecular biological methods against antibiotics of various classes. Antibiotic susceptibility testing against ampicillin, clindamycin, erythromycin, gentamicin, streptomycin, tetracycline and vancomycin has been performed. Minimal inhibitory concentrations (MICs) were determined by microdilution or Etest® techniques, selected strains were additionally tested by agar diffusion method. Furthermore, certain B. animalis, B. breve, B. pseudolongum and B. thermophilum strains were tested against chloramphenicol, kanamycin, linezolid, neomycin, streptogramin and trimethoprim using microdilution method. All strains of Bifidobacterium were sensitive to ampicillin, vancomycin, chloramphenicol, linezolid and streptogramin. The intrinsic resistance of bifidobacteria to aminoglycoside antibiotics (gentamicin, kanamycin, neomycin and streptomycin) could be verified. However, susceptibility to clindamycin, erythromycin, tetracycline and trimethoprim was variable. The greatest number of isolates (66,7 %) was resistant to tetracycline, seven strains showed cross-resistance against clindamycin and erythromycin. In addition to the five B. breve isolates tested, potentially acquired resistance against trimethoprim was found in 26 % of B. thermophilum isolates. Besides, trimethoprim exhibited a MIC range showing decreased susceptibility in B. pseudolongum and B. thermophilum. Moreover the strains were tested for the presence of selected gene classes encoding resistance to tetracycline and erythromycin using PCR techniques. No significant reduction of the incidence of resistant phenotypes could be noticed in the meat production chain (from farm to food retailing). However, samples isolated from the pig production chain showed increased occurrence of resistance compared to samples from cattle.

BetreuerIn: Kneifel Wolfgang
2.BetreuerIn: Domig Konrad

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