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Doktoratskolleg plus "Biomolecular Technology of Proteins"

Projektleitung
Obinger Christian, Projektleiter/in
Laufzeit:
01.04.2010-31.12.2018
Programm:
Doktoratskollegs plus
Art der Forschung
Grundlagenforschung
Mitarbeiter/innen
Altmann Friedrich, Sub-Projektleiter/in
Borth Nicole, Sub-Projektleiter/in
Haltrich Dietmar, Sub-Projektleiter/in
Jungbauer Alois, Sub-Projektleiter/in
Kunert Renate, Sub-Projektleiter/in
Schäffer Christina, Sub-Projektleiter/in
Steinkellner Herta, Sub-Projektleiter/in
Mattanovich Diethard, Sub-Projektleiter/in
Rüker Florian, Sub-Projektleiter/in
Vorauer-Uhl Karola, Sub-Projektleiter/in
Ludwig Roland, Sub-Projektleiter/in
Gasser Brigitte, Sub-Projektleiter/in
Grillari Johannes, Sub-Projektleiter/in
Strasser Richard, Sub-Projektleiter/in
Mach Lukas, Sub-Projektleiter/in
Laimer Margit, Sub-Projektleiter/in
Grabherr Reingard, Sub-Projektleiter/in
Wilson Iain B.H., Sub-Projektleiter/in
Köllensperger Gunda, Sub-Projektleiter/in
Stöger Eva, Sub-Projektleiter/in
Oostenbrink Chris, Sub-Projektleiter/in
Gübitz Georg, Sub-Projektleiter/in
Himmelbauer Heinz, Sub-Projektleiter/in
Beteiligte BOKU-Organisationseinheiten
Abteilung für Analytische Chemie
Abteilung für Biochemie
Institut für Angewandte Genetik und Zellbiologie (IAGZ)
Institut für Biologisch inspirierte Materialien
Institut für Biotechnologie
Institut für Lebensmitteltechnologie (LMT)
Institut für Molekulare Modellierung und Simulation (MMS)
Institut für Umweltbiotechnologie
Gefördert durch
Fonds zur Förderung der wissenschaftlichen Forschung (FWF) , Sensengasse 1, 1090 Wien, Österreich
Abstract
Die biotechnologische Produktion nativer und maßgeschneiderter rekombinanter Proteine ist ein komplexer Prozess. Er erfordert ein inter- und multidisziplinäres Ausbildungsprogramm, das neben biochemischen und zellbiologischen Grundlagen die Kenntnis von prokaryotischen und eukaryotischen Zellfabriken, von Bioinformatik und molekularer Modellierung sowie industrieller Umsetzung vermittelt. Das neue Doktoratskolleg BioToP (Biomolecular Technology of Proteins) integriert gezielt Grundlagenforschung und angewandte Forschung auf dem Gebiet moderner Proteinwissenschaften und Proteinproduktion für therapeutische, diagnostische und industrielle Anwendungen (vom Gen zum Produkt). Es bietet eine umfassende und topaktuelle forschungsgeleitete Ausbildung in den Fachgebieten (i) Struktur-Funktionsanalyse und Design von Proteinen, (ii) Synthese, Targeting und posttranslationale Modifikationen von Proteinen, (iii) Expressionssysteme und Zellfabriken, sowie (iv) Bioinformatik, Molekulare Modellierung und Downstream-processing.

BioToP wird für hoch qualifizierte weibliche und männliche Hochschulabsolventen aus aller Welt attraktiv sein. Die Studierenden werden die Möglichkeit haben, in einer von 14 Forschungsgruppen mit exzellenter wissenschaftlicher Kompetenz - belegt durch mehr als 420 SCI Publikationen im Zeitraum 2004 bis 2009 - zu arbeiten. Zusätzlich werden die Studierenden von bestehenden nationalen und europäischen Forschungsnetzwerken und industriellen Kompetenzzentren wie dem Austrian Center of Industrial Biotechnology und drei Christian Doppler Labors profitieren. Das internationale Doktoratsprogramm bietet eine exzellente, kreative und stimulierende Umgebung für Forschung und Ausbildung und wird die AbsolventInnen auf eine erfolgreiche Karriere in Grundlagenforschung und/oder angewandter Forschung vorbereiten.

BioToP ist ein gemeinsamer Antrag der BOKU-Departments für Angewandte Genetik und Zellbiologie, Biotechnologie, Chemie, Lebensmittelwissenschaften und –technologie, Materialwissenschaften und Prozesstechnik sowie Nanobiotechnologie, die gemeinsam das VIBT (Vienna Institute of BioTechnology) bilden. Aufgrund seines einzigartigen Forschungsclusters am Schnittpunkt von Grundlagenforschung und angewandter Forschung, der exzellenten Infrastruktur und der nötigen kritischen Masse an ForscherInnen bietet das VIBT den idealen Rahmen für eine stimulierende inter- und multidisziplinäre Ausbildung.
Schlagworte
Biomathematik; Computerunterstützte Simulation; Bioinformatik; Analytische Chemie; Biochemie; Biochemische Technologie; Strukturchemie; Biotechnologie; Enzymtechnologie; Spektroskopie (auch: Molekül-); Reaktionskinetik ; Strukturbiologie; Biokatalyse; Biochemie; Biophysik; Cytologie; Genetik; Mikrobiologie; Molekularbiologie; Phytotechnologie; Biotechnologie; Gentechnik, -technologie; Enzymtechnologie; Molekulare Nanotechnologie (Nanoengineering); Spektroskopie (auch: Molekül-); Proteomik; Strukturbiologie; Bioinformatik;
Bioinformatik und Molekular Modellierung; Biosynthese und post-translationale Modifikationen; Expressionssysteme und Zellfabriken; Proteinbiotechnologie; Struktur und Funktion von Proteinen;
Publikationen

Soudi, M., Zamocky, M., Furtmüller, PG., Obinger, C. (2011): Human peroxidasin 1 - phylogenetic analysis, cloning, expression and preliminary characterization .
[7th International Meeting on Human Peroxidases in Brussels, Belgium, May 22-25, , Brussels, May 22-25, 2011]

In: Van Antwerpen, P., 7th International Meeting on Human Peroxidases

** Mlynek, G; Sjoblom, B; Kostan, J; Fureder, S; Maixner, F; Gysel, K; Furtmuller, PG; Obinger, C; Wagner, M; Daims, H; Djinovic-Carugo, K Unexpected Diversity of Chlorite Dismutases: a Catalytically Efficient Dimeric Enzyme from Nitrobacter winogradsky.

J BACTERIOL. 2011; 193(10): 2408-2417. WoS PubMed PUBMED Central FullText FullText_BOKU

Graf, MM; Peterbauer, C; Haltrich, D (2011): Covalent Attachment of FAD and Vicinity of the Isoalloxazine in Pyranose Dehydrogenase. [Poster]

17th International Symposium on Flavins and Flavoproteins, JUL 24-29, 2011, Berkeley, California, USA

Kurz S, Dinglasan R, Wilson IBH (2011): Cloning, Expression and Characterisation of Glycosyltransferases from the Mosquito Anopheles gambiae. [Poster]

45. Jahrestagung der Österreichischen Gesellschaft für Tropenmedizin und Parasitologie, 11.2011, Wien

Kurz S, Dinglasan R, Wilson IBH (2011): Cloning, Expression and Characterisation of Glycosyltransferases from the Mosquito Anopheles gambiae. [Poster]
[Glyco XXI (21st International Symposium on Glycoconjugates), Wien, 21-26. August 2011]

, Glycoconjugate Journal, 28, 303-Abs273; ISSN 0282-0080

** Stampler, J; Bellei, M; Soudi, M; Gruber, C; Battistuzzi, G; Furtmüller, PG; Obinger, C; Manipulating the proximal triad His-Asn-Arg in human myeloperoxidase..

Arch Biochem Biophys. 2011; 516(1):21-28 WoS PubMed FullText FullText_BOKU

** Traxlmayr, MW; Faissner, M; Stadlmayr, G; Hasenhindl, C; Antes, B; Rüker, F; Obinger, C Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast..

Biochim Biophys Acta. 2012; 1824(4):542-549 WoS PubMed PUBMED Central FullText FullText_BOKU

Soudi, M., Zamocky, M., Jakopitsch, C., Furtmüller, P.G., and Obinger, C. (2012): Human peroxidasin 1 – Phylogeny and preliminary characterization of a peculiar multidomain protein. [Poster]
[9th International Conference on Protein Stability, Lisbon, May, 2-4.]

In: A.M. Azevedo and L.P. Fonseca (Eds.), 9th International Conference on Protein Stability - From molecular level to market applications, P14

** Hofbauer, S; Gysel, K; Mlynek, G; Kostan, J; Hagmüller, A; Daims, H; Furtmüller, PG; Djinovic-Carugo, K; Obinger, C Impact of subunit and oligomeric structure on the thermal and conformational stability of chlorite dismutases..

Biochim Biophys Acta. 2012; 1824(9):1031-1038 WoS PubMed PUBMED Central FullText FullText_BOKU

Graf, MM; Bren, U; Peterbauer, C; Oostenbrink, C; Haltrich, D (2012): Pyranose Dehydrogenase - Investigating D-Glucose Binding by Means of Molecular Dynamics Simulation. [Poster]

Trends in Enzymology, JUN 3-6, 2012, Goettingen, GERMANY

Graf, MM; Peterbauer, C; Haltrich, D (2012): Three different Pyranose Dehydrogenases from Agaricus meleagris – Heterologous Expression in Pichia pastoris, Purification and Characterization. [Poster]

OxiZymes, SEP 16-19, 2012, Marseille, FRANCE

** Zámocký, M; García-Fernández, Q; Gasselhuber, B; Jakopitsch, C; Furtmüller, PG; Loewen, PC; Fita, I; Obinger, C; Carpena, X; High conformational stability of secreted eukaryotic catalase-peroxidases: answers from first crystal structure and unfolding studies..

J Biol Chem. 2012; 287(38):32254-32262 WoS PubMed PUBMED Central FullText FullText_BOKU

** Traxlmayr, MW; Hasenhindl, C; Hackl, M; Stadlmayr, G; Rybka, JD; Borth, N; Grillari, J; Rüker, F; Obinger, C; Construction of a stability landscape of the CH3 domain of human IgG1 by combining directed evolution with high throughput sequencing..

J Mol Biol. 2012; 423(3):397-412 WoS PubMed PUBMED Central FullText FullText_BOKU

** Obinger, C Catalases and hydrogen peroxide metabolism..

Arch Biochem Biophys. 2012; 525(2):93-94 WoS PubMed FullText FullText_BOKU

** Soudi, M; Zamocky, M; Jakopitsch, C; Furtmüller, PG; Obinger, C; Molecular evolution, structure, and function of peroxidasins..

Chem Biodivers. 2012; 9(9):1776-1793 WoS PubMed PUBMED Central FullText FullText_BOKU

Krondorfer, I., Lipp, K., Haltrich, D. Peterbauer, C. K. (2012): Engineering of pyranose dehydrogenase for modified oxygen reactivity.
[Oxizymes 2012, Marseille, 16.09. - 19.09.2012]

In: Thierry Tron, Anne Lomascolo, Oxizymes in Marseille

Brugger, D., Peterbauer, C.K., Haltrich, D. (2012): Microtiter-plate-based screening assay for flavin oxidoreductases. [Poster]
[Oxizymes 2012, Marseille, 16.09. - 19.09.2012]

In: Thierry Tron, Anne Lomascolo, Oxizymes in Marseille

Gonaus, C., Peterbauer, C.K., Haltrich, D. (2012): Agaricus bisporus pyranose dehydrogenase - recombinant expression in Pichia pastoris and characterization. [Poster]
[Oxizymes 2012, Marseille, 16.09. - 19.09.2012]

In: Thierry Tron, Anne Lomascolo, Oxizymes in Marseille

Graf, M., Peterbauer, C.K., Haltrich, D. (2012): Three different pyranose dehydrogenases from Agaricus meleagris - heterologous expression in Pichia pastoris, purification and characterization. [Poster]
[Oxizymes 2012, Marseille, 16.09. - 19.09.2012]

In: Thierry Tron, Anne Lomascolo, Oxizymes in Marseille

Soudi, M. Zamocky, M. Jakopitsch., C. Delporte, C. Van Antwerpen, P. Furtmüller, P.G. & Obinger, C. (2012): Human peroxidasin 1 - phylogenetic analysis and characterization of a perculiar multidomain protein. [Poster]
[Oxizymes, Marseilles, 16.-19. Sept. 2012]

In: Thierry Tron & Anne Lomascolo, Oxizymes, P58

** Hofbauer, S; Bellei, M; Sündermann, A; Pirker, KF; Hagmüller, A; Mlynek, G; Kostan, J; Daims, H; Furtmüller, PG; Djinović-Carugo, K; Oostenbrink, C; Battistuzzi, G; Obinger, C; Redox thermodynamics of high-spin and low-spin forms of chlorite dismutases with diverse subunit and oligomeric structures..

Biochemistry. 2012; 51(47):9501-9512 WoS PubMed PUBMED Central FullText FullText_BOKU

** Traxlmayr, MW; Lobner, E; Antes, B; Kainer, M; Wiederkum, S; Hasenhindl, C; Stadlmayr, G; Rüker, F; Woisetschläger, M; Moulder, K; Obinger, C; Directed evolution of Her2/neu-binding IgG1-Fc for improved stability and resistance to aggregation by using yeast surface display..

Protein Eng Des Sel. 2013; 26(4):255-265 WoS PubMed FullText FullText_BOKU

Hofbauer, S., Flego, N., Schaffner, I., Pirker, K. F., Furtmüller, P. G., Howes, B. D., Obinger, C., Smulevich, G. (2013): Spectroscopic Characterization of wild-type Chlorite Dismutase from Candidatus “Nitrospira defluvii” and selected variants. [Poster]
[15th European Conference on the Spectroscopy of Biological Molecules, Oxford, UK, 25.-30. August]

In: European society for Spectroscopy of Biological Molecules , Conference on the Spectroscopy of Biological Molecules, http://www.ecsbm.eu/node/14

Auer, M. Gruber, C. Bellei, M., Pirker, K., Kroiß, D., Zamocky, M. Teufer, A. S., Soudi, M., Battistuzzi, G., Obinger, C. and Furtmüller P. G. (2013): New highly stable chlorinating bacterial peroxidase with autocatalytically formed covalent heme to protein bonds. [Poster]
[16th International Conference on Biological Inorganic Chemistry, ICBIC16, Grenoble, 22.-26. July ]

In: Marc Fontecave, 16th International Conference on Biological Inorganic Chemistry, http://icbic16.abstractcentral.com/

Hofbauer, S., Gruber, C., Pirker, K. F., Schaffner, I., Hagmüller, A., Gysel, K., Mlynek, G., Kostan, J., Bellei, M., Daims, H., Djinovic-Carugo, K. Battistuzzi, G., Furtmüller, P.G. and Obinger, C (2013): Understanding highly stable chlorite dismutase form nitrate-oxidizing bacterium Candidatus “Nitrospira defluvii”. [Poster]
[16th International Conference on Biological Inorganic Chemistry, ICBIC16, Grenoble, 22.-26. July ]

In: Marc Fontecave, 16th International Conference on Biological Inorganic Chemistry, http://icbic16.abstractcentral.com/

** Auer, M; Gruber, C; Bellei, M; Pirker, KF; Zamocky, M; Kroiss, D; Teufer, SA; Hofbauer, S; Soudi, M; Battistuzzi, G; Furtmüller, PG; Obinger, C; A stable bacterial peroxidase with novel halogenating activity and an autocatalytically linked heme prosthetic group..

J Biol Chem. 2013; 288(38):27181-27199 WoS PubMed PUBMED Central FullText FullText_BOKU

** Hasenhindl, C; Traxlmayr, MW; Wozniak-Knopp, G; Jones, PC; Stadlmayr, G; Rüker, F; Obinger, C; Stability assessment on a library scale: a rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc..

Protein Eng Des Sel. 2013; 26(10):675-682 WoS PubMed PUBMED Central FullText FullText_BOKU

Obinger, C., Grishkovskaya, I., Auer, M., Soudi, M., Paumann-Page, M., Zamocky, M., Djinovic-Carugo, K., Furtmüller, P. G. (2013): Evolution of structure and function in vertebrate heme peroxidases.
[8th International Human Peroxidase Meeting, Sydney, September 9-12]

In: Davies, M., 8th International Human Peroxidase Meeting, http://oxidation2013.org.au/

Soudi, M., Delporte, C., Paumann-Page, M., Van Antwerpen, P., Furtmüller, P. G., Obinger, C. (2013): Phylogenetic analyses and preliminary biochemical characterization of recombinant human peroxidasin 1.
[8th International Human Peroxidase Meeting, Sydney, September 9-12, 2013]

In: Davies, M., 8th International Human Peroxidase Meeting, http://oxidation2013.org.au/

** Paumann-Page, M; Furtmüller, PG; Hofbauer, S; Paton, LN; Obinger, C; Kettle, AJ; Inactivation of human myeloperoxidase by hydrogen peroxide..

Arch Biochem Biophys. 2013; 539(1):51-62 WoS PubMed PUBMED Central FullText FullText_BOKU

** Kurz, S; Jin, C; Hykollari, A; Gregorich, D; Giomarelli, B; Vasta, GR; Wilson, IB; Paschinger, K; Hemocytes and plasma of the eastern oyster (Crassostrea virginica) display a diverse repertoire of sulfated and blood group A-modified N-glycans..

J Biol Chem. 2013; 288(34):24410-24428 WoS PubMed PUBMED Central FullText FullText_BOKU

** Hofbauer, S; Gysel, K; Bellei, M; Hagmüller, A; Schaffner, I; Mlynek, G; Kostan, J; Pirker, KF; Daims, H; Furtmüller, PG; Battistuzzi, G; Djinović-Carugo, K; Obinger, C; Manipulating conserved heme cavity residues of chlorite dismutase: effect on structure, redox chemistry, and reactivity..

Biochemistry. 2014; 53(1):77-89 WoS PubMed PUBMED Central FullText FullText_BOKU

Kurz, S. (2013): Fucosylated glycan epitopes of the malaria mosquito Anopheles gambiae (short talk and poster).

Glycobiology Gordon Research Seminar and Conference, 2013, Ventura, CA

Kurz, S. (2013): Fucosylated glycan epitopes of the malaria mosquito Anopheles gambiae (Abs. 146).
[International Symposium on Glycoconjugates GLYCO XXII, Dalian, 23.-28.6.2013]

, GLYCOCONJUGATE JOURNAL, 30, 354-354; ISSN 0282-0080

Lai, B; Hasenhindl, C; Obinger, C; Oostenbrink, C; (2014): Molecular dynamics simulation of the crystallizable fragment of IgG1-insights for the design of Fcabs..

Int J Mol Sci. 2014; 15(1):438-455 PubMed PUBMED Central FullText FullText_BOKU

Nocon, J., Pfeffer, M., Sohn, S.B., Kim, T.Y., Maurer, M., Gasser, B., Lee, S.Y., Mattanovich, D. (2013): Metabolic model-based prediction of engineering targets for increased production of heterologous proteins.
[7th Conference on Recombinant Protein Production – RPP7, Laupheim, Germany, March 6-8, 2013]

In: European Federation of Biotechnology, RPP7-Abstract Book

Maccani, A., Tatto, N., Graf, A., Leitner, C., Mattanovich, D., Kunert, R., Ernst, W. (2013): Transcriptome study of high and low producing recombinant Chinese hamster ovary cell lines. [Poster]
[7th Conference on Recombinant Protein Production – RPP7, Laupheim, Germany, March 6-8, 2013]

In: European Federation of Biotechnology, RPP7-Abstract Book

Rebnegger, C; Graf, AB; Valli, M; Buchetics, M; Gasser, B; Maurer, M; Mattanovich, D. (2013): Regulation of recombinant protein secretion in relation to cell growth in the methylotrophic yeast Pichia pastoris . [Poster]
[7th Conference on Recombinant Protein Production – RPP7, Laupheim, Germany, March 6-8, 2013]

In: European Federation of Biotechnology, RPP7-Abstract Book

Nocon, J; Pfeffer, M; Sohn, SB; Kim, TY;, Maurer, M; Rußmayer, H; Steiger, M; Haberhauer-Troyer, C; Hann, S; Köllensperger, G;, Gasser, B; Lee, SY; Mattanovich, D. (2013): Metabolic model-based prediction of engineering targets for increased production of heterologous proteins . [Poster]

Quantitative Biology: Current concepts and tools for microbial strain and process development, July 15-19, 2013, Berlin, Germany

** Hofbauer, S; Schaffner, I; Furtmüller, PG; Obinger, C; Chlorite dismutases - a heme enzyme family for use in bioremediation and generation of molecular oxygen..

Biotechnol J. 2014; 9(4):461-473 WoS PubMed PUBMED Central FullText FullText_BOKU

** Jakopitsch, C; Pirker, KF; Flemmig, J; Hofbauer, S; Schlorke, D; Furtmüller, PG; Arnhold, J; Obinger, C; Mechanism of reaction of chlorite with mammalian heme peroxidases..

J Inorg Biochem. 2014; 135:10-19 WoS PubMed PUBMED Central FullText FullText_BOKU

** Obinger, C; Editorial: biomolecular technology of proteins - BioToP..

Biotechnol J. 2014; 9(4):453-454 WoS PubMed FullText FullText_BOKU

Hasenhindl, C., Traxlmayr, M.W., Wozniak-Knopp, G ., Jones, P.C., Stadlmayr, G., Rüker, F., Obinger, C. (2014): Stability assessment on a library scale – A rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc. .
[Human Antibodies & Hybridomas, Vienna, March 31- April 2]

In: Glassy, M., Human Antibodies & Hybridomas

Lobner, E., Traxlmayr, M. W., Rüker, F., Obinger, C. (2014): Directed evolution of antibody fragments for improved stability and resistance to aggregation by using yeast surface display. [Poster]
[Human Antibodies & Hybridomas, Vienna, Austria, March 31- April 2]

In: Glassy, M., Human Antibodies & Hybridomas

** Hofbauer, S; Gruber, C; Pirker, KF; Sündermann, A; Schaffner, I; Jakopitsch, C; Oostenbrink, C; Furtmüller, PG; Obinger, C; Transiently produced hypochlorite is responsible for the irreversible inhibition of chlorite dismutase..

Biochemistry. 2014; 53(19):3145-3157 WoS PubMed PUBMED Central FullText FullText_BOKU

** Hasenhindl, C; Lai, B; Delgado, J; Traxlmayr, MW; Stadlmayr, G; Rüker, F; Serrano, L; Oostenbrink, C; Obinger, C; Creating stable stem regions for loop elongation in Fcabs - insights from combining yeast surface display, in silico loop reconstruction and molecular dynamics simulations..

Biochim Biophys Acta. 2014; 1844(9):1530-1540 WoS PubMed PUBMED Central FullText FullText_BOKU

** Zámocký, M; Gasselhuber, B; Furtmüller, PG; Obinger, C; Turning points in the evolution of peroxidase-catalase superfamily: molecular phylogeny of hybrid heme peroxidases..

Cell Mol Life Sci. 2014; 71(23):4681-4696 WoS PubMed PUBMED Central FullText FullText_BOKU

** Anzengruber, J; Courtin, P; Claes, IJ; Debreczeny, M; Hofbauer, S; Obinger, C; Chapot-Chartier, MP; Vanderleyden, J; Messner, P; Schäffer, C; Biochemical characterization of the major N-acetylmuramidase from Lactobacillus buchneri..

Microbiology. 2014; 160(Pt 8):1807-1819 WoS PubMed PUBMED Central FullText FullText_BOKU

** Traxlmayr, MW; Lobner, E; Hasenhindl, C; Stadlmayr, G; Oostenbrink, C; Rüker, F; Obinger, C; Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution..

Biotechnol J. 2014; 9(8):1013-1022 WoS PubMed PUBMED Central FullText FullText_BOKU

** Sündermann, A; Reif, MM; Hofbauer, S; Obinger, C; Oostenbrink, C; Investigation of ion binding in chlorite dismutases by means of molecular dynamics simulations..

Biochemistry. 2014; 53(29):4869-4879 WoS PubMed PUBMED Central FullText FullText_BOKU

** Bort, JAH; Shanmukam, V; Pabst, M; Windwarder, M; Neumann, L; Alchalabi, A; Krebiehl, G; Koellensperger, G; Hann, S; Sonntag, D; Altmann, F; Heel, C; Borth, N Reduced quenching and extraction time for mammalian cells using filtration and syringe extraction.

J BIOTECHNOL. 2014; 182: 97-103. WoS FullText FullText_BOKU

** Auer, M; Nicolussi, A; Schütz, G; Furtmüller, PG; Obinger, C; How covalent heme to protein bonds influence the formation and reactivity of redox intermediates of a bacterial peroxidase..

J Biol Chem. 2014; 289(45):31480-31491 WoS PubMed PUBMED Central FullText FullText_BOKU

Schaffner, I., Hofbauer, S., Furtmueller, P. G., Obinger, C. (2014): Chlorite dismutases and dye-decolorizing peroxidases – similarities and differences within a structural superfamily of heme proteins.
[16th European Congress on Biotechnology, Edingburgh, July 13-16]

In: ECB, ECB16 (Ed.: Mitchell, W.). Talk.

Lobner, E., Traxlmayr, M. W., Rueker, F., Obinger, C. (2014): Construction of pH-sensitive Her2 binding antibody fragment by directed evolution using yeast display. [Poster]
[The 16th European Congress on Biotechnology, Ediburgh, July 13-16]

In: ECB, ECB16 (Ed.: Mitchell, W.)

** Schosserer, M; Minois, N; Angerer, TB; Amring, M; Dellago, H; Harreither, E; Calle-Perez, A; Pircher, A; Gerstl, MP; Pfeifenberger, S; Brandl, C; Sonntagbauer, M; Kriegner, A; Linder, A; Weinhäusel, A; Mohr, T; Steiger, M; Mattanovich, D; Rinnerthaler, M; Karl, T; Sharma, S; Entian, KD; Kos, M; Breitenbach, M; Wilson, IB; Polacek, N; Grillari-Voglauer, R; Breitenbach-Koller, L; Grillari, J; Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan..

Nat Commun. 2015; 6:6158 WoS PubMed PUBMED Central FullText FullText_BOKU

** Kurz, S; Aoki, K; Jin, C; Karlsson, NG; Tiemeyer, M; Wilson, IB; Paschinger, K; Targeted release and fractionation reveal glucuronylated and sulphated N- and O-glycans in larvae of dipteran insects..

J Proteomics. 2015; 126:172-188 WoS PubMed PUBMED Central FullText FullText_BOKU

Gasselhuber, B., Jakopitsch, C., Zamocky, M., Furtmüller, P. G., Obinger, C. (2015): Mechanistic aspects of catalase-peroxidase in HEME PEROXIDASES.

, Raven, E., Dunford H. B. , Gasselhuber, B., Jakopitsch, C., Zamocky, M., Furtmüller, P. G., Obinger, C. 2015. Mechanistic aspects of catalase-peroxidase. In Heme Peroxidases (Raven, E., Dunford, H. B.) Royal Society of Chemistry (RSC), Cambridge, pp. 156-180, DOI:10.1039/9781782622628-00156, ISBN: 978-1-84973-911-5, 156-180; Royal Chemical Society (RSC), Cambridge; ISBN: 978-1-84973-911-5

** Lobner, E; Traxlmayr, MW; Obinger, C; Hasenhindl, C; Engineered IgG1-Fc - one fragment to bind them all..

Immunol Rev. 2016; 270(1):-31 WoS PubMed PUBMED Central FullText FullText_BOKU

** Kurz, S; King, JG; Dinglasan, RR; Paschinger, K; Wilson, IB; The fucomic potential of mosquitoes: Fucosylated N-glycan epitopes and their cognate fucosyltransferases..

Insect Biochem Mol Biol. 2016; 68:52-63 WoS PubMed PUBMED Central FullText FullText_BOKU

Obinger, C., Jakopitsch, C., Furtmüller, P. G., Gasselhuber, B., Zamocky, M (2016): Mechanism of hydrogen peroxide to dioxygen oxidation by bifunctional catalase-peroxidase.
[79th Harden Conference on Oxygen Evolution and Reduction - Common Principles, Innsbruck, April 16-20]

In: Rich, P., Marechal, A., MacMillan, F., Brudvig, G., Oxygen Evolution and Reduction - Common Principles, British Biochemical Society, www.biochemistry.org

Hofbauer, S., Grünwald-Gruber, C., Schaffner, I., Sündermann, A., Jakopitsch, C., Oostenbrink, C., Djinovic-Carugo, K., Furtmüller, P.G., Obinger, C. (2016): Dioxygen evolution by chlorite dismutase depends on co-ordination of transiently produced hypochlorite to an oxoiron(IV) intermediate.
[79th Harden Conference on Oxygen Evolution and Reduction, Innsbruck, April 16-20]

In: Rich, P., Marechal, A., MacMillan, F., Brudvig, G., Oxygen Evolution and Reduction, British Biochemical Society, www.biochemistry.org

Schaffner, I., Mlynek, G., Hofbauer, S., Smulevich, G., Battistuzzi, G., Djinovic-Carugo, K., Furtmüller, P. G., Coates, L., Obinger, C. (2016): High-resultion X-ray and neutron diffration structures and biophysical studies on a novel dimeric chlorite dismutase. [Poster]
[79th Harden Conference on Oxygen Evolution and Reduction - Common Principles, Innsbruck, April 16-20]

In: Rich, P., Marechal, A., MacMillan, F., Brudvig, G., Oxygen Evolution and Reduction - Common Principles, British Biochemical Society, www.biochemistry.com

Lobner, E., Humm, A.-S., Göritzer, K., Mlynek, G., Hasenhindl, C., Traxlmayr, M., Djinoviv-Carugo, K., Obinger, C. (2016): Structures and thermodynamics of Fcab-antigen complexes.. [Poster]
[PEGS, Boston, April 25-29]

In: CHI, Cambridge Innovation Institute, PEGS 2016, http://pegssummit.com/

** Hofbauer, S; Howes, BD; Flego, N; Pirker, KF; Schaffner, I; Mlynek, G; Djinović-Carugo, K; Furtmüller, PG; Smulevich, G; Obinger, C; From chlorite dismutase towards HemQ - the role of the proximal H-bonding network in haeme binding..

Biosci Rep. 2016; 36(2): WoS PubMed PUBMED Central FullText FullText_BOKU

** Gasselhuber, B; Graf, MMH; Jakopitsch, C; Zamocky, M; Nicolussi, A; Furtmuller, PG; Oostenbrink, C; Carpena, X; Obinger, C Interaction with the Redox Cofactor MYW and Functional Role of a Mobile Arginine in Eukaryotic Catalase-Peroxidase.

BIOCHEMISTRY-US. 2016; 55(25): 3528-3541. WoS PubMed FullText FullText_BOKU

** Hofbauer, S; Dalla Sega, M; Scheiblbrandner, S; Jandova, Z; Schaffner, I; Mlynek, G; Djinović-Carugo, K; Battistuzzi, G; Furtmüller, PG; Oostenbrink, C; Obinger, C; Chemistry and Molecular Dynamics Simulations of Heme b-HemQ and Coproheme-HemQ..

Biochemistry. 2016; 55(38):5398-5412 WoS PubMed PUBMED Central FullText FullText_BOKU

Obinger, C., Auer, M., Nicolussi, A., Paumann-Page, M., Soudi, M., Furtmüller, P. G. (2016): Impact of heme-protein crosslinking on catalysis of prokaryotic and eukaryotic peroxidases.
[13th European Biological Inorganic Chemistry Conference (EuroBIC), Budapest, Aug. 28 – Sept. 1]

In: Hungarian Chemical Society (Eds. Kiss, T., Sovago, I.), EuroBIC 13

Schaffner, I., Hofbauer, S., Mlynek, G., Battistuzzi, G., Djinovic-Carugo, K., Furtmüller, P. G., Obinger, C. (2016): Chlorite dismutase: distal heme environment and its role in irreversible enzyme inactivation. [Poster]
[13th European Biological Inorganic Chemistry Conference (EuroBIC), Budapest Aug. 28 – Sept. 1, , Budapest, Aug. 28 – Sept. 1 ]

In: Hungarian Chemical Society (Eds. Kiss, T., Sovago, I.), EuroBIC 13

Hofbauer, S., Scheiblbrandner, S., Schaffner, I., Djinović-Carugo, K., Battistuzzi, G., Furtmüller, P. G., Obinger, C. (2016): Coproheme and HemQ. [Poster]
[13th European Biological Inorganic Chemistry Conference (EuroBIC) Aug. 28 – Sept. 1, Budapest, Aug. 28 – Sept. 1]

In: Hungarian Chemical Society (Eds. Kiss, T., Sovago, I.), EuroBIC13

Furtmüller, P. G., Nicolussi, A., Auer. M., Weissensteiner, J., Bellei, M., Battistuzzi, G., Obinger, C. (2016): Influence of covalent heme to protein bonds in redox reactions of Lyngbia peroxidase . [Poster]
[13th European Biological Inorganic Chemistry Conference (EuroBIC) , Budapest, Aug. 28 – Sept. 1]

In: Hungarian Chemical Society (Eds. Kiss, T., Sovago, I.), EuroBIC

Jakopitsch, C., Gasselhuber, B., Furtmüller, P. G., Obinger, C. (2016): Two redox active cofactors are essential for the catalatic activity of catalase-peroxidases.
[13th European Biological Inorganic Chemistry Conference (EuroBIC) Aug. 28 – Sept. 1, poster, Budapest, Aug. 28 – Sept. 1, poster]

In: Hungarian Chemical Society (Eds. Kiss, T., Sovago, I.), EuroBIC 13

Sevcnikar, B., Soudi, M., Paumann-Page, M., Furtmüller, P. G., Obinger, C. (2016): Human peroxidasin 1: A multidomain heme peroxidase . [Poster]
[13th European Biological Inorganic Chemistry Conference (EuroBIC) , Budapest, August 28 - September 1]

In: Hungarian Chemical Society (Eds. Kiss, T., Sovago, I.), EuroBIC

Pfanzagl, V., Furtmüller, P. G., Obinger, C. 2016. Dye-decolorizing peroxidases – a new heme-peroxidase family with ancient roots (2016): Dye-decolorizing peroxidases – a new heme-peroxidase family with ancient roots. [Poster]
[13th European Biological Inorganic Chemistry Conference (EuroBIC) , Budapest, Aug. 28 – Sept. 1]

In: Hungarian Chemical Society (Kiss, T., Sovago, I.), EuroBIC13

Goeritzer K., Strasser, R., Obinger, C. (2016): Expression, purification and characterization of monomeric IgA1 and IgA2 directed against HER2 in Nicotiana benthamiana.
[European Congress on Biotechnology, Krakow, July 3-6]

In: Elsevier, New Biotechnology 33S, S72; ISBN: 1871-6784

** Hofbauer, S; Mlynek, G; Milazzo, L; Pühringer, D; Maresch, D; Schaffner, I; Furtmüller, PG; Smulevich, G; Djinović-Carugo, K; Obinger, C; Hydrogen peroxide-mediated conversion of coproheme to heme b by HemQ-lessons from the first crystal structure and kinetic studies..

FEBS J. 2016; 283(23):4386-4401 WoS PubMed FullText FullText_BOKU

Pereira Aguilar, P; Schneider, T; Jungbauer, A (2016): Adsorption and elution of enveloped Virus-Like Particles in polymer grafted chromatography media. [Poster]

ISPPP2016 International Symposium on the Separation of Proteins, Peptides and Polynucleotides, NOV 6 – 9, 2016, Salzburg, AUSTRIA

Pereira Aguilar, P; Schneider, T; Jungbauer, A (2016): Adsorption and Transport of Virus-Like Particles into Charged Hydrogels and Chromatography Beads. [Poster]

BioProcess International Conference & Exhibition, APR 11-14, 2016, Vienna, AUSTRIA

Pereira Aguilar, P; Schneider, T; Jungbauer, A (2016): Adsorption and transport of virus-like particles in charged hydrogel and chromatography beads. [Poster]

European Symposium on Biochemical Engineering Sciences (ESBES) 2016, SEP 11-14 2016, , Dublin, AUSTRIA

Pereira Aguilar, P; Schneider, T; Jungbauer, A (2016): Virus-like particles adsorption in anion exchange chromatography media virus-like particles . [Poster]

Vaccine Technology VI, , JUN 12-17, 2016,, Albufeira, Portugal

** Paumann-Page, M; Katz, RS; Bellei, M; Schwartz, I; Edenhofer, E; Sevcnikar, B; Soudi, M; Hofbauer, S; Battistuzzi, G; Furtmüller, PG; Obinger, C; Pre-steady-state Kinetics Reveal the Substrate Specificity and Mechanism of Halide Oxidation of Truncated Human Peroxidasin 1..

J Biol Chem. 2017; 292(11):4583-4592 WoS PubMed FullText FullText_BOKU

** Pfanzagl, V; Schaffner, I; Hofbauer, S; Furtmuller, P; Obinger, C Dye decolorizing peroxidases - A new heme-peroxidase family with ancient roots.
[European Congress on Biotechnology, Krakow, July 3-6]

NEW BIOTECHNOL. 2016; 33: S4-S4. WoS FullText FullText_BOKU

** Grishkovskaya, I; Paumann-Page, M; Tscheliessnig, R; Stampler, J; Hofbauer, S; Soudi, M; Sevcnikar, B; Oostenbrink, C; Furtmüller, PG; Djinović-Carugo, K; Nauseef, WM; Obinger, C; Structure of human promyeloperoxidase (proMPO) and the role of the propeptide in processing and maturation..

J Biol Chem. 2017; 292(20):8244-8261 WoS PubMed PUBMED Central FullText FullText_BOKU

** Lobner, E; Humm, AS; Göritzer, K; Mlynek, G; Puchinger, MG; Hasenhindl, C; Rüker, F; Traxlmayr, MW; Djinović-Carugo, K; Obinger, C; Fcab-HER2 Interaction: a Ménage à Trois. Lessons from X-Ray and Solution Studies..

Structure. 2017; 25(6):878-889.e5 WoS PubMed FullText FullText_BOKU

** Göritzer, K; Maresch, D; Altmann, F; Obinger, C; Strasser, R; Exploring Site-Specific N-Glycosylation of HEK293 and Plant-Produced Human IgA Isotypes..

J Proteome Res. 2017; 16(7):2560-2570 WoS PubMed FullText FullText_BOKU

** Nicolussi, A; Auer, M; Weissensteiner, J; Schütz, G; Katz, S; Maresch, D; Hofbauer, S; Bellei, M; Battistuzzi, G; Furtmüller, PG; Obinger, C; Posttranslational Modification of Heme b in a Bacterial Peroxidase: The Role of Heme to Protein Ester Bonds in Ligand Binding and Catalysis..

Biochemistry. 2017; 56(34):4525-4538 WoS PubMed FullText FullText_BOKU

** Lobner, E; Humm, AS; Mlynek, G; Kubinger, K; Kitzmüller, M; Traxlmayr, MW; Djinović-Carugo, K; Obinger, C; Two-faced Fcab prevents polymerization with VEGF and reveals thermodynamics and the 2.15 Å crystal structure of the complex..

MAbs. 2017; 9(7):1088-1104 WoS PubMed FullText FullText_BOKU

Pfanzagl, V; Hofbauer, S; Nys, K; Djinović-Carugo, K; Battistuzzi, G; Van Doorslaer, S; Furtmüller, P.G; and Obinger, C; (2017): Roles of the distal Asp-Arg pair in compound I formation of K. pneumoniae dye-decolorizing peroxidase . [Poster]
[ICBIC18 – International Conference on Biological Inorganic Chemistry, Florianópolis, July, 31.– Aug. 4. 2017 ]

In: A.Neves, M. da Costa Ferreira, A.J. Vila (Eds.), ICBIC18 – International Conference on Biological Inorganic Chemistry

Nicolussi, A., Dunn, J. D., Bellei, M., Zamocky, M., Furtmueller, P. G., Battistuzzi, G., Soldati, T., Obinger, C. (2017): Biochemical and in-vivo investigations of a peroxidase from the social amoeba Dictyostelium discoideum.
[Life Science 2 meeting, Zürich, February 2 – 3]

In: von Schoultz, C., LS 2 Meeting 2017

** Schaffner, I; Mlynek, G; Flego, N; Pühringer, D; Libiseller-Egger, J; Coates, L; Hofbauer, S; Bellei, M; Furtmüller, PG; Battistuzzi, G; Smulevich, G; Djinović-Carugo, K; Obinger, C; Molecular Mechanism of Enzymatic Chlorite Detoxification: Insights from Structural and Kinetic Studies..

ACS Catal. 2017; 7(11):7962-7976 WoS PubMed PUBMED Central FullText FullText_BOKU

** Nicolussi, A; Dunn, JD; Mlynek, G; Bellei, M; Zamocky, M; Battistuzzi, G; Djinović-Carugo, K; Furtmüller, PG; Soldati, T; Obinger, C; Secreted heme peroxidase from Dictyostelium discoideum: Insights into catalysis, structure, and biological role..

J Biol Chem. 2018; 293(4):1330-1345 WoS PubMed FullText FullText_BOKU

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