Doktoratskolleg plus "Biomolecular Technology of Proteins"

Oostenbrink Chris

Institute of Molecular Modeling and Simulation (MMS)

01.04.2010 - 31.12.2023

Doctoral school

Program Doktoratskollegs plus

Funder Austrian Science Fund (FWF)

Competencies

Biotechnologie
Nanowissenschaften und -technologie

Abstract

Recombinant production of native and engineered proteins by biotechnological and biopharmaceutical means is a complex process that requires an inter- and multidisciplinary educational training in fields ranging from basic biochemistry and cell biology to production systems based on different organisms of varying complexity, bioinformatics and downstream processing. The doctoral program BioToP (Biomolecular Technology of Proteins) will integrate basic and applied research in the field of modern recombinant protein science and production (from gene to product). It is a joint application of the BOKU Departments of Applied Genetics and Cell Biology, Biotechnology, Chemistry, Food Sciences and Technology, Material Sciences and Process Engineering and Nanobiotechnology, which together constitute the VIBT (Vienna Institute of Biotechnology) of BOKU. In detail, BioToP will provide comprehensive and thorough up-to-date research training in the fields of (i) analysis, design and engineering of proteins, (ii) biosynthesis, posttranslational modifications and trafficking of (recombinant) proteins, (iii) expression systems and cell factories, and (iv) bioinformatics and molecular modelling. The already existing unique cluster of basic and applied research represented by scientists in the field of biotechnology at BOKU provides an excellent infrastructure, the critical mass and a stimulating training environment to facilitate interdisciplinary and translational research. BioToP will provide the unifying educational platform for an international PhD program that will attract highly qualified graduate students from all over the world. Students selected by a competitive and transparent admission procedure will have the opportunity to work in any of 14 research teams with excellent scientific competence and infrastructure as indicated by more than 420 SCI publications and 54 approved FWF projects within the period 2004-2009. Additionally, students will benefit from several existing national and European research networks and industrial competence centres coordinated by BOKU, e.g. Austrian Center for Biopharmaceutical Technology and three Christian Doppler Laboratories. Alltogether this will provide an excellent, creative and stimulating environment for research and educational training, and prepare PhD students for a successful career in basic and/or applied research.

Protein Biotechnology
Biosynthesis and posttranslational modifications
Expression systems and cell factories
Bioinformatics and molecular modelling
Structure function of proteins

Publications

Human peroxidasin 1 - phylogenetic analysis, cloning, expression and preliminary characterization

Autoren: Soudi, M., Zamocky, M., Furtmüller, PG., Obinger, C. Jahr: 2011

Conference & Workshop proceedings, paper, abstract
Unexpected Diversity of Chlorite Dismutases: a Catalytically Efficient Dimeric Enzyme from Nitrobacter winogradsky

Autoren: Mlynek, G; Sjoblom, B; Kostan, J; Fureder, S; Maixner, F; Gysel, K; Furtmuller, PG; Obinger, C; Wagner, M; Daims, H; Djinovic-Carugo, K Jahr: 2011

Journal articles
Cloning, Expression and Characterisation of Glycosyltransferases from the Mosquito Anopheles gambiae

Autoren: Kurz S, Dinglasan R, Wilson IBH Jahr: 2011

Journal articles
Manipulating the proximal triad His-Asn-Arg in human myeloperoxidase.

Autoren: Stampler, J; Bellei, M; Soudi, M; Gruber, C; Battistuzzi, G; Furtmüller, PG; Obinger, C; Jahr: 2011

Journal articles
Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast.

Autoren: Traxlmayr, MW; Faissner, M; Stadlmayr, G; Hasenhindl, C; Antes, B; Rüker, F; Obinger, C Jahr: 2012

Journal articles
Human peroxidasin 1 – Phylogeny and preliminary characterization of a peculiar multidomain protein

Autoren: Soudi, M., Zamocky, M., Jakopitsch, C., Furtmüller, P.G., and Obinger, C. Jahr: 2012

Conference & Workshop proceedings, paper, abstract
Impact of subunit and oligomeric structure on the thermal and conformational stability of chlorite dismutases.

Autoren: Hofbauer, S; Gysel, K; Mlynek, G; Kostan, J; Hagmüller, A; Daims, H; Furtmüller, PG; Djinovic-Carugo, K; Obinger, C Jahr: 2012

Journal articles
High conformational stability of secreted eukaryotic catalase-peroxidases: answers from first crystal structure and unfolding studies.

Autoren: Zámocký, M; García-Fernández, Q; Gasselhuber, B; Jakopitsch, C; Furtmüller, PG; Loewen, PC; Fita, I; Obinger, C; Carpena, X; Jahr: 2012

Journal articles
Construction of a stability landscape of the CH3 domain of human IgG1 by combining directed evolution with high throughput sequencing.

Autoren: Traxlmayr, MW; Hasenhindl, C; Hackl, M; Stadlmayr, G; Rybka, JD; Borth, N; Grillari, J; Rüker, F; Obinger, C; Jahr: 2012

Journal articles
Catalases and hydrogen peroxide metabolism.

Autoren: Obinger, C Jahr: 2012

Journal articles
Molecular evolution, structure, and function of peroxidasins.

Autoren: Soudi, M; Zamocky, M; Jakopitsch, C; Furtmüller, PG; Obinger, C; Jahr: 2012

Journal articles
Engineering of pyranose dehydrogenase for modified oxygen reactivity

Autoren: Krondorfer, I., Lipp, K., Haltrich, D. Peterbauer, C. K. Jahr: 2012

Conference & Workshop proceedings, paper, abstract
Microtiter-plate-based screening assay for flavin oxidoreductases

Autoren: Brugger, D., Peterbauer, C.K., Haltrich, D. Jahr: 2012

Conference & Workshop proceedings, paper, abstract
Agaricus bisporus pyranose dehydrogenase - recombinant expression in Pichia pastoris and characterization

Autoren: Gonaus, C., Peterbauer, C.K., Haltrich, D. Jahr: 2012

Conference & Workshop proceedings, paper, abstract
Three different pyranose dehydrogenases from Agaricus meleagris - heterologous expression in Pichia pastoris, purification and characterization

Autoren: Graf, M., Peterbauer, C.K., Haltrich, D. Jahr: 2012

Conference & Workshop proceedings, paper, abstract
Human peroxidasin 1 - phylogenetic analysis and characterization of a perculiar multidomain protein

Autoren: Soudi, M. Zamocky, M. Jakopitsch., C. Delporte, C. Van Antwerpen, P. Furtmüller, P.G. & Obinger, C. Jahr: 2012

Conference & Workshop proceedings, paper, abstract
Redox thermodynamics of high-spin and low-spin forms of chlorite dismutases with diverse subunit and oligomeric structures.

Autoren: Hofbauer, S; Bellei, M; Sündermann, A; Pirker, KF; Hagmüller, A; Mlynek, G; Kostan, J; Daims, H; Furtmüller, PG; Djinović-Carugo, K; Oostenbrink, C; Battistuzzi, G; Obinger, C; Jahr: 2012

Journal articles
Directed evolution of Her2/neu-binding IgG1-Fc for improved stability and resistance to aggregation by using yeast surface display.

Autoren: Traxlmayr, MW; Lobner, E; Antes, B; Kainer, M; Wiederkum, S; Hasenhindl, C; Stadlmayr, G; Rüker, F; Woisetschläger, M; Moulder, K; Obinger, C; Jahr: 2013

Journal articles
Spectroscopic Characterization of wild-type Chlorite Dismutase from Candidatus “Nitrospira defluvii” and selected variants

Autoren: Hofbauer, S., Flego, N., Schaffner, I., Pirker, K. F., Furtmüller, P. G., Howes, B. D., Obinger, C., Smulevich, G. Jahr: 2013

Conference & Workshop proceedings, paper, abstract
New highly stable chlorinating bacterial peroxidase with autocatalytically formed covalent heme to protein bonds

Autoren: Auer, M. Gruber, C. Bellei, M., Pirker, K., Kroiß, D., Zamocky, M. Teufer, A. S., Soudi, M., Battistuzzi, G., Obinger, C. and Furtmüller P. G. Jahr: 2013

Conference & Workshop proceedings, paper, abstract
Understanding highly stable chlorite dismutase form nitrate-oxidizing bacterium Candidatus “Nitrospira defluvii”

Autoren: Hofbauer, S., Gruber, C., Pirker, K. F., Schaffner, I., Hagmüller, A., Gysel, K., Mlynek, G., Kostan, J., Bellei, M., Daims, H., Djinovic-Carugo, K. Battistuzzi, G., Furtmüller, P.G. and Obinger, C Jahr: 2013

Conference & Workshop proceedings, paper, abstract
A stable bacterial peroxidase with novel halogenating activity and an autocatalytically linked heme prosthetic group.

Autoren: Auer, M; Gruber, C; Bellei, M; Pirker, KF; Zamocky, M; Kroiss, D; Teufer, SA; Hofbauer, S; Soudi, M; Battistuzzi, G; Furtmüller, PG; Obinger, C; Jahr: 2013

Journal articles
Stability assessment on a library scale: a rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc.

Autoren: Hasenhindl, C; Traxlmayr, MW; Wozniak-Knopp, G; Jones, PC; Stadlmayr, G; Rüker, F; Obinger, C; Jahr: 2013

Journal articles
Evolution of structure and function in vertebrate heme peroxidases

Autoren: Obinger, C., Grishkovskaya, I., Auer, M., Soudi, M., Paumann-Page, M., Zamocky, M., Djinovic-Carugo, K., Furtmüller, P. G. Jahr: 2013

Conference & Workshop proceedings, paper, abstract
Phylogenetic analyses and preliminary biochemical characterization of recombinant human peroxidasin 1

Autoren: Soudi, M., Delporte, C., Paumann-Page, M., Van Antwerpen, P., Furtmüller, P. G., Obinger, C. Jahr: 2013

Conference & Workshop proceedings, paper, abstract
Inactivation of human myeloperoxidase by hydrogen peroxide.

Autoren: Paumann-Page, M; Furtmüller, PG; Hofbauer, S; Paton, LN; Obinger, C; Kettle, AJ; Jahr: 2013

Journal articles
Hemocytes and plasma of the eastern oyster (Crassostrea virginica) display a diverse repertoire of sulfated and blood group A-modified N-glycans.

Autoren: Kurz, S; Jin, C; Hykollari, A; Gregorich, D; Giomarelli, B; Vasta, GR; Wilson, IB; Paschinger, K; Jahr: 2013

Journal articles
Manipulating conserved heme cavity residues of chlorite dismutase: effect on structure, redox chemistry, and reactivity.

Autoren: Hofbauer, S; Gysel, K; Bellei, M; Hagmüller, A; Schaffner, I; Mlynek, G; Kostan, J; Pirker, KF; Daims, H; Furtmüller, PG; Battistuzzi, G; Djinović-Carugo, K; Obinger, C; Jahr: 2014

Journal articles
Fucosylated glycan epitopes of the malaria mosquito Anopheles gambiae (Abs. 146)

Autoren: Kurz, S. Jahr: 2013

Journal articles
Metabolic model-based prediction of engineering targets for increased production of heterologous proteins

Autoren: Nocon, J., Pfeffer, M., Sohn, S.B., Kim, T.Y., Maurer, M., Gasser, B., Lee, S.Y., Mattanovich, D. Jahr: 2013

Conference & Workshop proceedings, paper, abstract
Transcriptome study of high and low producing recombinant Chinese hamster ovary cell lines

Autoren: Maccani, A., Tatto, N., Graf, A., Leitner, C., Mattanovich, D., Kunert, R., Ernst, W. Jahr: 2013

Conference & Workshop proceedings, paper, abstract
Regulation of recombinant protein secretion in relation to cell growth in the methylotrophic yeast Pichia pastoris

Autoren: Rebnegger, C; Graf, AB; Valli, M; Buchetics, M; Gasser, B; Maurer, M; Mattanovich, D. Jahr: 2013

Conference & Workshop proceedings, paper, abstract
Chlorite dismutases - a heme enzyme family for use in bioremediation and generation of molecular oxygen.

Autoren: Hofbauer, S; Schaffner, I; Furtmüller, PG; Obinger, C; Jahr: 2014

Journal articles
Mechanism of reaction of chlorite with mammalian heme peroxidases.

Autoren: Jakopitsch, C; Pirker, KF; Flemmig, J; Hofbauer, S; Schlorke, D; Furtmüller, PG; Arnhold, J; Obinger, C; Jahr: 2014

Journal articles
Editorial: biomolecular technology of proteins - BioToP.

Autoren: Obinger, C; Jahr: 2014

Journal articles
Stability assessment on a library scale – A rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc.

Autoren: Hasenhindl, C., Traxlmayr, M.W., Wozniak-Knopp, G ., Jones, P.C., Stadlmayr, G., Rüker, F., Obinger, C. Jahr: 2014

Conference & Workshop proceedings, paper, abstract
Directed evolution of antibody fragments for improved stability and resistance to aggregation by using yeast surface display

Autoren: Lobner, E., Traxlmayr, M. W., Rüker, F., Obinger, C. Jahr: 2014

Conference & Workshop proceedings, paper, abstract
Transiently produced hypochlorite is responsible for the irreversible inhibition of chlorite dismutase.

Autoren: Hofbauer, S; Gruber, C; Pirker, KF; Sündermann, A; Schaffner, I; Jakopitsch, C; Oostenbrink, C; Furtmüller, PG; Obinger, C; Jahr: 2014

Journal articles
Creating stable stem regions for loop elongation in Fcabs - insights from combining yeast surface display, in silico loop reconstruction and molecular dynamics simulations.

Autoren: Hasenhindl, C; Lai, B; Delgado, J; Traxlmayr, MW; Stadlmayr, G; Rüker, F; Serrano, L; Oostenbrink, C; Obinger, C; Jahr: 2014

Journal articles
Turning points in the evolution of peroxidase-catalase superfamily: molecular phylogeny of hybrid heme peroxidases.

Autoren: Zámocký, M; Gasselhuber, B; Furtmüller, PG; Obinger, C; Jahr: 2014

Journal articles
Biochemical characterization of the major N-acetylmuramidase from Lactobacillus buchneri.

Autoren: Anzengruber, J; Courtin, P; Claes, IJ; Debreczeny, M; Hofbauer, S; Obinger, C; Chapot-Chartier, MP; Vanderleyden, J; Messner, P; Schäffer, C; Jahr: 2014

Journal articles
Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution.

Autoren: Traxlmayr, MW; Lobner, E; Hasenhindl, C; Stadlmayr, G; Oostenbrink, C; Rüker, F; Obinger, C; Jahr: 2014

Journal articles
Investigation of ion binding in chlorite dismutases by means of molecular dynamics simulations.

Autoren: Sündermann, A; Reif, MM; Hofbauer, S; Obinger, C; Oostenbrink, C; Jahr: 2014

Journal articles
Reduced quenching and extraction time for mammalian cells using filtration and syringe extraction

Autoren: Bort, JAH; Shanmukam, V; Pabst, M; Windwarder, M; Neumann, L; Alchalabi, A; Krebiehl, G; Koellensperger, G; Hann, S; Sonntag, D; Altmann, F; Heel, C; Borth, N Jahr: 2014

Journal articles
How covalent heme to protein bonds influence the formation and reactivity of redox intermediates of a bacterial peroxidase.

Autoren: Auer, M; Nicolussi, A; Schütz, G; Furtmüller, PG; Obinger, C; Jahr: 2014

Journal articles
Chlorite dismutases and dye-decolorizing peroxidases – similarities and differences within a structural superfamily of heme proteins

Autoren: Schaffner, I., Hofbauer, S., Furtmueller, P. G., Obinger, C. Jahr: 2014

Conference & Workshop proceedings, paper, abstract
Construction of pH-sensitive Her2 binding antibody fragment by directed evolution using yeast display

Autoren: Lobner, E., Traxlmayr, M. W., Rueker, F., Obinger, C. Jahr: 2014

Conference & Workshop proceedings, paper, abstract
Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan.

Autoren: Schosserer, M; Minois, N; Angerer, TB; Amring, M; Dellago, H; Harreither, E; Calle-Perez, A; Pircher, A; Gerstl, MP; Pfeifenberger, S; Brandl, C; Sonntagbauer, M; Kriegner, A; Linder, A; Weinhäusel, A; Mohr, T; Steiger, M; Mattanovich, D; Rinnerthaler, M; Karl, T; Sharma, S; Entian, KD; Kos, M; Breitenbach, M; Wilson, IB; Polacek, N; Grillari-Voglauer, R; Breitenbach-Koller, L; Grillari, J; Jahr: 2015

Journal articles
Targeted release and fractionation reveal glucuronylated and sulphated N- and O-glycans in larvae of dipteran insects.

Autoren: Kurz, S; Aoki, K; Jin, C; Karlsson, NG; Tiemeyer, M; Wilson, IB; Paschinger, K; Jahr: 2015

Journal articles
Mechanistic aspects of catalase-peroxidase in HEME PEROXIDASES

Autoren: Gasselhuber, B., Jakopitsch, C., Zamocky, M., Furtmüller, P. G., Obinger, C. Jahr: 2015

Chapter in collected volumes
Engineered IgG1-Fc--one fragment to bind them all.

Autoren: Lobner, E; Traxlmayr, MW; Obinger, C; Hasenhindl, C; Jahr: 2016

Journal articles
The fucomic potential of mosquitoes: Fucosylated N-glycan epitopes and their cognate fucosyltransferases.

Autoren: Kurz, S; King, JG; Dinglasan, RR; Paschinger, K; Wilson, IB; Jahr: 2016

Journal articles
Mechanism of hydrogen peroxide to dioxygen oxidation by bifunctional catalase-peroxidase

Autoren: Obinger, C., Jakopitsch, C., Furtmüller, P. G., Gasselhuber, B., Zamocky, M Jahr: 2016

Conference & Workshop proceedings, paper, abstract
Dioxygen evolution by chlorite dismutase depends on co-ordination of transiently produced hypochlorite to an oxoiron(IV) intermediate

Autoren: Hofbauer, S., Grünwald-Gruber, C., Schaffner, I., Sündermann, A., Jakopitsch, C., Oostenbrink, C., Djinovic-Carugo, K., Furtmüller, P.G., Obinger, C. Jahr: 2016

Conference & Workshop proceedings, paper, abstract
High-resultion X-ray and neutron diffration structures and biophysical studies on a novel dimeric chlorite dismutase

Autoren: Schaffner, I., Mlynek, G., Hofbauer, S., Smulevich, G., Battistuzzi, G., Djinovic-Carugo, K., Furtmüller, P. G., Coates, L., Obinger, C. Jahr: 2016

Conference & Workshop proceedings, paper, abstract
Structures and thermodynamics of Fcab-antigen complexes.

Autoren: Lobner, E., Humm, A.-S., Göritzer, K., Mlynek, G., Hasenhindl, C., Traxlmayr, M., Djinoviv-Carugo, K., Obinger, C. Jahr: 2016

Conference & Workshop proceedings, paper, abstract
From chlorite dismutase towards HemQ - the role of the proximal H-bonding network in haeme binding.

Autoren: Hofbauer, S; Howes, BD; Flego, N; Pirker, KF; Schaffner, I; Mlynek, G; Djinović-Carugo, K; Furtmüller, PG; Smulevich, G; Obinger, C; Jahr: 2016

Journal articles
Interaction with the Redox Cofactor MYW and Functional Role of a Mobile Arginine in Eukaryotic Catalase-Peroxidase

Autoren: Gasselhuber, B; Graf, MMH; Jakopitsch, C; Zamocky, M; Nicolussi, A; Furtmuller, PG; Oostenbrink, C; Carpena, X; Obinger, C Jahr: 2016

Journal articles
Chemistry and Molecular Dynamics Simulations of Heme b-HemQ and Coproheme-HemQ.

Autoren: Hofbauer, S; Dalla Sega, M; Scheiblbrandner, S; Jandova, Z; Schaffner, I; Mlynek, G; Djinović-Carugo, K; Battistuzzi, G; Furtmüller, PG; Oostenbrink, C; Obinger, C; Jahr: 2016

Journal articles
Impact of heme-protein crosslinking on catalysis of prokaryotic and eukaryotic peroxidases

Autoren: Obinger, C., Auer, M., Nicolussi, A., Paumann-Page, M., Soudi, M., Furtmüller, P. G. Jahr: 2016

Conference & Workshop proceedings, paper, abstract
Chlorite dismutase: distal heme environment and its role in irreversible enzyme inactivation

Autoren: Schaffner, I., Hofbauer, S., Mlynek, G., Battistuzzi, G., Djinovic-Carugo, K., Furtmüller, P. G., Obinger, C. Jahr: 2016

Conference & Workshop proceedings, paper, abstract
Coproheme and HemQ

Autoren: Hofbauer, S., Scheiblbrandner, S., Schaffner, I., Djinović-Carugo, K., Battistuzzi, G., Furtmüller, P. G., Obinger, C. Jahr: 2016

Conference & Workshop proceedings, paper, abstract
Influence of covalent heme to protein bonds in redox reactions of Lyngbia peroxidase

Autoren: Furtmüller, P. G., Nicolussi, A., Auer. M., Weissensteiner, J., Bellei, M., Battistuzzi, G., Obinger, C. Jahr: 2016

Conference & Workshop proceedings, paper, abstract
Two redox active cofactors are essential for the catalatic activity of catalase-peroxidases

Autoren: Jakopitsch, C., Gasselhuber, B., Furtmüller, P. G., Obinger, C. Jahr: 2016

Conference & Workshop proceedings, paper, abstract
Human peroxidasin 1: A multidomain heme peroxidase

Autoren: Sevcnikar, B., Soudi, M., Paumann-Page, M., Furtmüller, P. G., Obinger, C. Jahr: 2016

Conference & Workshop proceedings, paper, abstract
Dye-decolorizing peroxidases – a new heme-peroxidase family with ancient roots

Autoren: Pfanzagl, V., Furtmüller, P. G., Obinger, C. 2016. Dye-decolorizing peroxidases – a new heme-peroxidase family with ancient roots Jahr: 2016

Conference & Workshop proceedings, paper, abstract
Expression, purification and characterization of monomeric IgA1 and IgA2 directed against HER2 in Nicotiana benthamiana

Autoren: Goeritzer K., Strasser, R., Obinger, C. Jahr: 2016

Conference & Workshop proceedings, paper, abstract
Hydrogen peroxide-mediated conversion of coproheme to heme b by HemQ-lessons from the first crystal structure and kinetic studies.

Autoren: Hofbauer, S; Mlynek, G; Milazzo, L; Pühringer, D; Maresch, D; Schaffner, I; Furtmüller, PG; Smulevich, G; Djinović-Carugo, K; Obinger, C; Jahr: 2016

Journal articles
Pre-steady-state Kinetics Reveal the Substrate Specificity and Mechanism of Halide Oxidation of Truncated Human Peroxidasin 1.

Autoren: Paumann-Page, M; Katz, RS; Bellei, M; Schwartz, I; Edenhofer, E; Sevcnikar, B; Soudi, M; Hofbauer, S; Battistuzzi, G; Furtmüller, PG; Obinger, C; Jahr: 2017

Journal articles
Dye decolorizing peroxidases - A new heme-peroxidase family with ancient roots

Autoren: Pfanzagl, V; Schaffner, I; Hofbauer, S; Furtmuller, P; Obinger, C Jahr: 2016

Journal articles
Structure of human promyeloperoxidase (proMPO) and the role of the propeptide in processing and maturation.

Autoren: Grishkovskaya, I; Paumann-Page, M; Tscheliessnig, R; Stampler, J; Hofbauer, S; Soudi, M; Sevcnikar, B; Oostenbrink, C; Furtmüller, PG; Djinović-Carugo, K; Nauseef, WM; Obinger, C; Jahr: 2017

Journal articles
Fcab-HER2 Interaction: a Ménage à Trois. Lessons from X-Ray and Solution Studies.

Autoren: Lobner, E; Humm, AS; Göritzer, K; Mlynek, G; Puchinger, MG; Hasenhindl, C; Rüker, F; Traxlmayr, MW; Djinović-Carugo, K; Obinger, C; Jahr: 2017

Journal articles
Exploring Site-Specific N-Glycosylation of HEK293 and Plant-Produced Human IgA Isotypes.

Autoren: Göritzer, K; Maresch, D; Altmann, F; Obinger, C; Strasser, R; Jahr: 2017

Journal articles
Posttranslational Modification of Heme b in a Bacterial Peroxidase: The Role of Heme to Protein Ester Bonds in Ligand Binding and Catalysis.

Autoren: Nicolussi, A; Auer, M; Weissensteiner, J; Schütz, G; Katz, S; Maresch, D; Hofbauer, S; Bellei, M; Battistuzzi, G; Furtmüller, PG; Obinger, C; Jahr: 2017

Journal articles
Two-faced Fcab prevents polymerization with VEGF and reveals thermodynamics and the 2.15 Å crystal structure of the complex.

Autoren: Lobner, E; Humm, AS; Mlynek, G; Kubinger, K; Kitzmüller, M; Traxlmayr, MW; Djinović-Carugo, K; Obinger, C; Jahr: 2017

Journal articles
Roles of the distal Asp-Arg pair in compound I formation of K. pneumoniae dye-decolorizing peroxidase

Autoren: Pfanzagl, V; Hofbauer, S; Nys, K; Djinović-Carugo, K; Battistuzzi, G; Van Doorslaer, S; Furtmüller, P.G; and Obinger, C; Jahr: 2017

Conference & Workshop proceedings, paper, abstract
Biochemical and in-vivo investigations of a peroxidase from the social amoeba Dictyostelium discoideum

Autoren: Nicolussi, A., Dunn, J. D., Bellei, M., Zamocky, M., Furtmueller, P. G., Battistuzzi, G., Soldati, T., Obinger, C. Jahr: 2017

Conference & Workshop proceedings, paper, abstract
Molecular Mechanism of Enzymatic Chlorite Detoxification: Insights from Structural and Kinetic Studies.

Autoren: Schaffner, I; Mlynek, G; Flego, N; Pühringer, D; Libiseller-Egger, J; Coates, L; Hofbauer, S; Bellei, M; Furtmüller, PG; Battistuzzi, G; Smulevich, G; Djinović-Carugo, K; Obinger, C; Jahr: 2017

Journal articles
Secreted heme peroxidase from Dictyostelium discoideum: Insights into catalysis, structure, and biological role.

Autoren: Nicolussi, A; Dunn, JD; Mlynek, G; Bellei, M; Zamocky, M; Battistuzzi, G; Djinović-Carugo, K; Furtmüller, PG; Soldati, T; Obinger, C; Jahr: 2018

Journal articles
Posttranslational modification of heme in peroxidases - Impact on structure and catalysis.

Autoren: Nicolussi, A; Auer, M; Sevcnikar, B; Paumann-Page, M; Pfanzagl, V; Zámocký, M; Hofbauer, S; Furtmüller, PG; Obinger, C; Jahr: 2018

Journal articles
Myeloperoxidase-catalyzed oxidation of cyanide to cyanate: A potential carbamylation route involved in the formation of atherosclerotic plaques?

Autoren: Delporte, C; Zouaoui Boudjeltia, K; Furtmüller, PG; Maki, RA; Dieu, M; Noyon, C; Soudi, M; Dufour, D; Coremans, C; Nuyens, V; Reye, F; Rousseau, A; Raes, M; Moguilevsky, N; Vanhaeverbeek, M; Ducobu, J; Nève, J; Robaye, B; Vanhamme, L; Reynolds, WF; Obinger, C; Van Antwerpen, P; Jahr: 2018

Journal articles
Insights into the Active Site of Coproheme Decarboxylase from Listeria monocytogenes.

Autoren: Milazzo, L; Hofbauer, S; Howes, BD; Gabler, T; Furtmüller, PG; Obinger, C; Smulevich, G; Jahr: 2018

Journal articles
Crystal and Electronic Structure of Compound I in Dye-decolorizing Peroxidases

Autoren: Pfanzagl, V; Hofbauer, S; Beale, J; Mlynek, G; Michlits, H; Nys, K; Van Doorslaer, S; Djinovic-Carugo, K; Furtmüller, PG; Obinger, C Jahr: 2018

Conference & Workshop proceedings, paper, abstract
Human peroxidasin 1 promotes angiogenesis through ERK1/2, Akt, and FAK pathways.

Autoren: Medfai, H; Khalil, A; Rousseau, A; Nuyens, V; Paumann-Page, M; Sevcnikar, B; Furtmüller, PG; Obinger, C; Moguilevsky, N; Peulen, O; Herfs, M; Castronovo, V; Amri, M; Van Antwerpen, P; Vanhamme, L; Zouaoui Boudjeltia, K; Jahr: 2019

Journal articles
Characterization and comparison of the distal heme environment of dimeric and pentameric chlorite dismutases

Autoren: Schmidt, D., Schaffner, I., Hofbauer, S., Mlynek, G., Djinović-Carugo, K., Furtmüller, P. G., Obinger, C. Jahr: 2018

Conference & Workshop proceedings, paper, abstract
Kinetic and spectroscopic characterization of EfeB

Autoren: Michlits, H., Pfanzagl, V., Schwaiger, L., Furtmüller, P. G., Hofbauer, S., Obinger, C. Jahr: 2018

Conference & Workshop proceedings, paper, abstract
On Compound I formation in B-type dye decolorizing peroxidases - lessons from structural and kinetic studies.

Autoren: Pfanzagl, V., Nys, K., Mlynek, G., Djinović-Carugo, K., Van Doorslaer, S., Furtmüller, P. G., Hofbauer, S., Obinger, C. Jahr: 2018

Conference & Workshop proceedings, paper, abstract
Coproheme decarboxylases – phylogenetic prediction versus biochemical analysis.

Autoren: Pfanzagl, V., Holcik, L., Maresch, D., Michlits, H., Furtmüller, P. G., Obinger, C., Hofbauer, S. Jahr: 2018

Conference & Workshop proceedings, paper, abstract
Roles of distal aspartate and arginine of B-class dye-decolorizing peroxidase in heterolytic hydrogen peroxide cleavage.

Autoren: Pfanzagl, V; Nys, K; Bellei, M; Michlits, H; Mlynek, G; Battistuzzi, G; Djinovic-Carugo, K; Van Doorslaer, S; Furtmüller, PG; Hofbauer, S; Obinger, C; Jahr: 2018

Journal articles
Isomeric Separation and Recognition of Anionic and Zwitterionic N-glycans from Royal Jelly Glycoproteins

Autoren: Hykollari, A; Malzl, D; Eckmair, B; Vanbeselaere, J; Scheidl, P; Jin, CS; Karlsson, NG; Wilson, IBH; Paschinger, K Jahr: 2018

Journal articles
Highly modified and immunoactive N-glycans of the canine heartworm

Autoren: Martini, F; Eckmair, B; Stefanic, S; Jin, CS; Garg, M; Yan, S; Jimenez-Castells, C; Hykollari, A; Neupert, C; Venco, L; Silva, DV; Wilson, IBH; Paschinger, K Jahr: 2019

Journal articles
The hydrogen bonding network of coproheme in coproheme decarboxylase from Listeria monocytogenes: Effect on structure and catalysis.

Autoren: Milazzo, L; Gabler, T; Pfanzagl, V; Michlits, H; Furtmüller, PG; Obinger, C; Hofbauer, S; Smulevich, G; Jahr: 2019

Journal articles
Monomeric and homotrimeric solution structures of truncated human peroxidasin 1 variants.

Autoren: Paumann-Page, M; Tscheliessnig, R; Sevcnikar, B; Katz, RS; Schwartz, I; Hofbauer, S; Pfanzagl, V; Furtmüller, PG; Obinger, C; Jahr: 2020

Journal articles
Redox thermodynamics of B-class dye-decolorizing peroxidases.

Autoren: Pfanzagl, V; Bellei, M; Hofbauer, S; Laurent, CVFP; Furtmüller, PG; Oostenbrink, C; Battistuzzi, G; Obinger, C; Jahr: 2019

Journal articles
Distinct Fcα receptor N-glycans modulate the binding affinity to immunoglobulin A (IgA) antibodiesRole of IgA and FcαRI N-glycans

Autoren: Göritzer, K; Turupcu, A; Maresch, D; Novak, J; Altmann, F; Oostenbrink, C; Obinger, C; Strasser, R; Jahr: 2019

Journal articles
Redox Cofactor Rotates during Its Stepwise Decarboxylation: Molecular Mechanism of Conversion of Coproheme to Heme

Autoren: Milazzo, L; Gabler, T; Pühringer, D; Jandova, Z; Maresch, D; Michlits, H; Pfanzagl, V; Djinović-Carugo, K; Oostenbrink, C; Furtmüller, PG; Obinger, C; Smulevich, G; Hofbauer, S; Jahr: 2019

Journal articles
Tissue-specific glycosylation in the honeybee: Analysis of the N-glycomes of Apis mellifera larvae and venom

Autoren: Hykollari, A; Malzl, D; Stanton, R; Eckmair, B; Paschinger, K Jahr: 2019

Journal articles
Reaction of human peroxidasin 1 compound I and compound II with one-electron donors.

Autoren: Sevcnikar, B; Paumann-Page, M; Hofbauer, S; Pfanzagl, V; Furtmüller, PG; Obinger, C; Jahr: 2020

Journal articles
Actinobacterial Coproheme Decarboxylases Use Histidine as a Distal Base to Promote Compound I Formation.

Autoren: Michlits, H; Lier, B; Pfanzagl, V; Djinović-Carugo, K; Furtmüller, PG; Oostenbrink, C; Obinger, C; Hofbauer, S; Jahr: 2020

Journal articles
The leucine-rich repeat domain of human peroxidasin 1 promotes binding to laminin in basement membranes.

Autoren: Sevcnikar, B; Schaffner, I; Chuang, CY; Gamon, L; Paumann-Page, M; Hofbauer, S; Davies, MJ; Furtmüller, PG; Obinger, C; Jahr: 2020

Journal articles
X-ray-induced photoreduction of heme metal centers rapidly induces active-site perturbations in a protein-independent manner.

Autoren: Pfanzagl, V; Beale, JH; Michlits, H; Schmidt, D; Gabler, T; Obinger, C; Djinović-Carugo, K; Hofbauer, S; Jahr: 2020

Journal articles
Understanding molecular enzymology of porphyrin-binding α + β barrel proteins - One fold, multiple functions.

Autoren: Hofbauer, S; Pfanzagl, V; Michlits, H; Schmidt, D; Obinger, C; Furtmüller, PG; Jahr: 2021

Journal articles
Arresting the Catalytic Arginine in Chlorite Dismutases: Impact on Heme Coordination, Thermal Stability, and Catalysis.

Autoren: Schmidt, D; Serra, I; Mlynek, G; Pfanzagl, V; Hofbauer, S; Furtmüller, PG; Djinović-Carugo, K; Van Doorslaer, S; Obinger, C; Jahr: 2021

Journal articles
Reaction intermediate rotation during the decarboxylation of coproheme to heme b in C. diphtheriae.

Autoren: Sebastiani, F; Michlits, H; Lier, B; Becucci, M; Furtmüller, PG; Oostenbrink, C; Obinger, C; Hofbauer, S; Smulevich, G; Jahr: 2021

Journal articles
Substrate specificity and complex stability of coproporphyrin ferrochelatase is governed by hydrogen-bonding interactions of the four propionate groups.

Autoren: Gabler, T; Sebastiani, F; Helm, J; Dali, A; Obinger, C; Furtmüller, PG; Smulevich, G; Hofbauer, S; Jahr: 2022

Journal articles
Impact of the dynamics of the catalytic arginine on nitrite and chlorite binding by dimeric chlorite dismutase.

Autoren: Serra, I; Schmidt, D; Pfanzagl, V; Mlynek, G; Hofbauer, S; Djinović-Carugo, K; Furtmüller, PG; García-Rubio, I; Van Doorslaer, S; Obinger, C; Jahr: 2022

Journal articles
An active site at work - the role of key residues in C. diphteriae coproheme decarboxylase.

Autoren: Sebastiani, F; Risorti, R; Niccoli, C; Michlits, H; Becucci, M; Hofbauer, S; Smulevich, G; Jahr: 2022

Journal articles
Initial Steps to Engineer Coproheme Decarboxylase to Obtain Stereospecific Monovinyl, Monopropionyl Deuterohemes.

Autoren: Michlits, H; Valente, N; Mlynek, G; Hofbauer, S; Jahr: 2021

Journal articles
Spectroscopic evidence of the effect of hydrogen peroxide excess on the coproheme decarboxylase from actinobacterial Corynebacterium diphtheriae

Autoren: Sebastiani, F; Niccoli, C; Michlits, H; Risorti, R; Becucci, M; Hofbauer, S; Smulevich, G Jahr: 2022

Journal articles
X-ray induced reduction of heme metal centers is protein-independent

Autoren: Pfanzagl, V; Beale, JH; Michlits, H; Schmidt, D; Gabler, T; Obinger, C; Djinovic-Carugo, K; Hofbauer, S; Jahr: 2022

Conference & Workshop proceedings, paper, abstract
The staphylococcal inhibitory protein SPIN binds to human myeloperoxidase with picomolar affinity but only dampens halide oxidation.

Autoren: Leitgeb, U; Furtmüller, PG; Hofbauer, S; Brito, JA; Obinger, C; Pfanzagl, V; Jahr: 2022

Journal articles
Active site architecture of coproporphyrin ferrochelatase with its physiological substrate coproporphyrin III: Propionate interactions and porphyrin core deformation

Autoren: Dali, A; Gabler, T; Sebastiani, F; Destinger, A; Furtmuller, PG; Pfanzagl, V; Becucci, M; Smulevich, G; Hofbauer, S Jahr: 2023

Journal articles
N-glycan antennal modifications are altered in Caenorhabditis elegans lacking the HEX-4 N-acetylgalactosamine-specific hexosaminidase.

Autoren: Paschinger, K; Wöls, F; Yan, S; Jin, C; Vanbeselaere, J; Dutkiewicz, Z; Arcalis, E; Malzl, D; Wilson, IBH; Jahr: 2023

Journal articles
Increasing Complexity of the N-Glycome During Caenorhabditis Development.

Autoren: Wilson, IBH; Yan, S; Jin, C; Dutkiewicz, Z; Rendić, D; Palmberger, D; Schnabel, R; Paschinger, K; Jahr: 2023

Journal articles
The role of the distal cavity in carbon monoxide stabilization in the coproheme decarboxylase enzyme from C. diphtheriae.

Autoren: Sebastiani, F; Dali, A; Alonso de Armiño, DJ; Campagni, L; Patil, G; Becucci, M; Hofbauer, S; Estrin, DA; Smulevich, G; Jahr: 2023

Journal articles
Reactivity of Coproheme Decarboxylase with Monovinyl, Monopropionate Deuteroheme.

Autoren: Patil, G; Michlits, H; Furtmüller, PG; Hofbauer, S; Jahr: 2023

Journal articles
Structural aspects of enzymes involved in prokaryotic Gram-positive heme biosynthesis.

Autoren: Falb, N; Patil, G; Furtmüller, PG; Gabler, T; Hofbauer, S; Jahr: 2023

Journal articles
Iron insertion into coproporphyrin III-ferrochelatase complex: Evidence for an intermediate distorted catalytic species.

Autoren: Gabler, T; Dali, A; Sebastiani, F; Furtmüller, PG; Becucci, M; Hofbauer, S; Smulevich, G; Jahr: 2023

Journal articles

Project staff

Christian Obinger

Univ.Prof. Mag. Dr.rer.nat. Christian Obinger
christian.obinger@boku.ac.at
Tel: +43 1 47654-10011, 77273

Project Leader

01.04.2010 - 31.12.2018

Sub Projectleader

01.01.2019 - 31.12.2023

Chris Oostenbrink

Univ.Prof. Dr. Chris Oostenbrink
chris.oostenbrink@boku.ac.at
Tel: +43 1 47654-89401, 89411, 89419

Project Leader

01.01.2019 - 31.12.2023

Sub Projectleader

01.04.2010 - 31.12.2018

Friedrich Altmann

Ao.Univ.Prof.i.R. Dipl.-Ing. Dr.nat.techn. Friedrich Altmann
friedrich.altmann@boku.ac.at
Tel: +43 1 47654-77262

Sub Projectleader

01.04.2010 - 31.12.2023

Nicole Borth

Ao.Univ.Prof. Dipl.-Ing. Dr.nat.techn. Nicole Borth
nicole.borth@boku.ac.at
Tel: +43 1 47654-79064, 79065

Sub Projectleader

01.04.2010 - 31.12.2023

Brigitte Gasser

Assoc. Prof. Priv.-Doz. Dipl.-Ing. Dr.nat.techn. Brigitte Gasser
brigitte.gasser@boku.ac.at
Tel: +43 1 47654-79033

Sub Projectleader

01.04.2010 - 31.12.2023

Reingard Grabherr

Univ.Prof. Dipl.-Ing. Dr.rer.nat. Reingard Grabherr
reingard.grabherr@boku.ac.at
Tel: +43 1 47654-79856

Sub Projectleader

01.04.2010 - 31.12.2023

Johannes Grillari

Assoc. Prof. Dr. Johannes Grillari
johannes.grillari@boku.ac.at
Tel: +43 1 47654-79067

Sub Projectleader

01.04.2010 - 31.12.2023

Georg Gübitz

Univ.Prof. Dipl.-Ing. Dr.techn. Georg Gübitz
guebitz@boku.ac.at
Tel: +43 1 47654-97001, 97402

Sub Projectleader

01.04.2010 - 31.12.2018

Dietmar Haltrich

Univ.Prof. Dipl.-Ing. Dr.techn. Dietmar Haltrich
dietmar.haltrich@boku.ac.at
Tel: +43 1 47654-75211

Sub Projectleader

01.04.2010 - 31.12.2023

Stephan Hann

Univ.Prof. Dr. Stephan Hann
stephan.hann@boku.ac.at
Tel: +43 1 47654-77001, 77101, 77191

Sub Projectleader

01.01.2019 - 31.12.2023

Heinz Himmelbauer

Univ.Prof. Mag. Dr.rer.nat. Heinz Himmelbauer
heinz.himmelbauer@boku.ac.at
Tel: +43 1 47654-79155

Sub Projectleader

01.04.2010 - 31.12.2018

Stefan Hofbauer

Ass.Prof. Priv.-Doz. Dipl.-Ing. Stefan Hofbauer Ph.D.
stefan.hofbauer@boku.ac.at
Tel: +43 1 47654-77258

Sub Projectleader

07.09.2021 - 31.12.2023

Alois Jungbauer

Univ.Prof. Dipl.-Ing. Dr.nat.techn. Alois Jungbauer
alois.jungbauer@boku.ac.at
Tel: +43 1 47654-79096

Sub Projectleader

01.04.2010 - 31.12.2023

Renate Kunert

Univ.Prof. Dipl.-Ing. Dr.nat.techn. Renate Kunert
renate.kunert@boku.ac.at
Tel: +43 1 47654-79852

Sub Projectleader

01.04.2010 - 31.12.2023

Gunda Köllensperger

Ao.Univ.Prof. Dipl.-Ing. Dr.nat.techn. Gunda Köllensperger
gunda.koellensperger@boku.ac.at

Sub Projectleader

01.04.2010 - 31.12.2023

Margit Laimer

Ao.Univ.Prof.i.R. Dr.phil. Margit Laimer
margit.laimer@boku.ac.at
Tel: +43 1 47654-79010

Sub Projectleader

01.04.2010 - 31.12.2023

Roland Ludwig

Assoc. Prof. Dipl.-Ing. Dr. Roland Ludwig
roland.ludwig@boku.ac.at
Tel: +43 1 47654-75216

Sub Projectleader

01.04.2010 - 31.12.2023

Lukas Mach

Univ.Prof. Dipl.-Ing. Dr. Lukas Mach
lukas.mach@boku.ac.at
Tel: +43 1 47654-94065, 94360

Sub Projectleader

01.04.2010 - 31.12.2023

Diethard Mattanovich

Univ.Prof. Dipl.-Ing. Dr.nat.techn. Diethard Mattanovich
diethard.mattanovich@boku.ac.at
Tel: +43 1 47654-79006

Sub Projectleader

01.04.2010 - 31.12.2023

Clemens Karl Peterbauer

Assoc. Prof. Dr. Clemens Karl Peterbauer
clemens.peterbauer@boku.ac.at
Tel: +43 1 47654-75212

Sub Projectleader

01.01.2019 - 31.12.2022

Florian Rüker

Ao.Univ.Prof.i.R. Dr. Florian Rüker
florian.rueker@boku.ac.at
Tel: +43 1 47654-79854

Sub Projectleader

01.04.2010 - 31.12.2023

Michael Sauer

Assoc. Prof. Priv.-Doz. Dipl.Natw. ETH FH-Prof. Dr. Michael Sauer
michael.sauer@boku.ac.at

Sub Projectleader

01.01.2019 - 31.12.2023

Christina Schäffer

Univ.Prof. Dipl.-Ing. Dr.nat.techn. Christina Schäffer
christina.schaeffer@boku.ac.at
Tel: +43 1 47654-80203

Sub Projectleader

01.04.2010 - 31.12.2023

Herta Steinkellner

Ao.Univ.Prof.i.R. Mag.rer.nat. Dr.nat.techn. Herta Steinkellner
herta.steinkellner@boku.ac.at
Tel: +43 1 47654-94370

Sub Projectleader

01.04.2010 - 31.12.2023

Richard Strasser

Assoc. Prof. Dr. Richard Strasser
richard.strasser@boku.ac.at
Tel: +43 1 47654-94345

Sub Projectleader

01.04.2010 - 31.12.2023

Eva Stöger

Univ.Prof. Mag. Dr.rer.nat. Eva Stöger
eva.stoeger@boku.ac.at
Tel: +43 1 47654-94001, 94311

Sub Projectleader

01.04.2010 - 31.12.2023

Michael Traxlmayr

Priv.-Doz. Dipl.-Ing. Dr. Michael Traxlmayr
michael.traxlmayr@boku.ac.at
Tel: +43 1 47654-77274

Sub Projectleader

14.06.2022 - 31.12.2023

Rupert Tscheließnig

Dipl.-Ing. Dr. Rupert Tscheließnig

Sub Projectleader

01.01.2019 - 31.12.2022

Karola Vorauer-Uhl

Ao.Univ.Prof. Dipl.-Ing. Dr.nat.techn. Karola Vorauer-Uhl
karola.vorauer-uhl@boku.ac.at
Tel: +43 1 47654-79850

Sub Projectleader

01.04.2010 - 31.12.2023

Iain B.H. Wilson

Ao.Univ.Prof. Dr.phil. Iain B.H. Wilson
iain.wilson@boku.ac.at
Tel: +43 1 47654-77216, 77217

Sub Projectleader

01.04.2010 - 31.12.2023

Hans Jürgen Zanghellini

Dipl.-Ing. Dr. Hans Jürgen Zanghellini
juergen.zanghellini@boku.ac.at

Sub Projectleader

01.01.2019 - 31.12.2023

Doktoratskolleg plus "Biomolecular Technology of Proteins"

Abstract

Publications

Human peroxidasin 1 - phylogenetic analysis, cloning, expression and preliminary characterization

Unexpected Diversity of Chlorite Dismutases: a Catalytically Efficient Dimeric Enzyme from Nitrobacter winogradsky

Cloning, Expression and Characterisation of Glycosyltransferases from the Mosquito Anopheles gambiae

Manipulating the proximal triad His-Asn-Arg in human myeloperoxidase.

Directed evolution of stabilized IgG1-Fc scaffolds by application of strong heat shock to libraries displayed on yeast.

Human peroxidasin 1 – Phylogeny and preliminary characterization of a peculiar multidomain protein

Impact of subunit and oligomeric structure on the thermal and conformational stability of chlorite dismutases.

High conformational stability of secreted eukaryotic catalase-peroxidases: answers from first crystal structure and unfolding studies.

Construction of a stability landscape of the CH3 domain of human IgG1 by combining directed evolution with high throughput sequencing.

Catalases and hydrogen peroxide metabolism.

Molecular evolution, structure, and function of peroxidasins.

Engineering of pyranose dehydrogenase for modified oxygen reactivity

Microtiter-plate-based screening assay for flavin oxidoreductases

Agaricus bisporus pyranose dehydrogenase - recombinant expression in Pichia pastoris and characterization

Three different pyranose dehydrogenases from Agaricus meleagris - heterologous expression in Pichia pastoris, purification and characterization

Human peroxidasin 1 - phylogenetic analysis and characterization of a perculiar multidomain protein

Redox thermodynamics of high-spin and low-spin forms of chlorite dismutases with diverse subunit and oligomeric structures.

Directed evolution of Her2/neu-binding IgG1-Fc for improved stability and resistance to aggregation by using yeast surface display.

Spectroscopic Characterization of wild-type Chlorite Dismutase from Candidatus “Nitrospira defluvii” and selected variants

New highly stable chlorinating bacterial peroxidase with autocatalytically formed covalent heme to protein bonds

Understanding highly stable chlorite dismutase form nitrate-oxidizing bacterium Candidatus “Nitrospira defluvii”

A stable bacterial peroxidase with novel halogenating activity and an autocatalytically linked heme prosthetic group.

Stability assessment on a library scale: a rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc.

Evolution of structure and function in vertebrate heme peroxidases

Phylogenetic analyses and preliminary biochemical characterization of recombinant human peroxidasin 1

Inactivation of human myeloperoxidase by hydrogen peroxide.

Hemocytes and plasma of the eastern oyster (Crassostrea virginica) display a diverse repertoire of sulfated and blood group A-modified N-glycans.

Manipulating conserved heme cavity residues of chlorite dismutase: effect on structure, redox chemistry, and reactivity.

Fucosylated glycan epitopes of the malaria mosquito Anopheles gambiae (Abs. 146)

Metabolic model-based prediction of engineering targets for increased production of heterologous proteins

Transcriptome study of high and low producing recombinant Chinese hamster ovary cell lines

Regulation of recombinant protein secretion in relation to cell growth in the methylotrophic yeast Pichia pastoris

Chlorite dismutases - a heme enzyme family for use in bioremediation and generation of molecular oxygen.

Mechanism of reaction of chlorite with mammalian heme peroxidases.

Editorial: biomolecular technology of proteins - BioToP.

Stability assessment on a library scale – A rapid method for the evaluation of the commutability and insertion of residues in C-terminal loops of the CH3 domains of IgG1-Fc.

Directed evolution of antibody fragments for improved stability and resistance to aggregation by using yeast surface display

Transiently produced hypochlorite is responsible for the irreversible inhibition of chlorite dismutase.

Creating stable stem regions for loop elongation in Fcabs - insights from combining yeast surface display, in silico loop reconstruction and molecular dynamics simulations.

Turning points in the evolution of peroxidase-catalase superfamily: molecular phylogeny of hybrid heme peroxidases.

Biochemical characterization of the major N-acetylmuramidase from Lactobacillus buchneri.

Construction of pH-sensitive Her2-binding IgG1-Fc by directed evolution.

Investigation of ion binding in chlorite dismutases by means of molecular dynamics simulations.

Reduced quenching and extraction time for mammalian cells using filtration and syringe extraction

How covalent heme to protein bonds influence the formation and reactivity of redox intermediates of a bacterial peroxidase.

Chlorite dismutases and dye-decolorizing peroxidases – similarities and differences within a structural superfamily of heme proteins

Construction of pH-sensitive Her2 binding antibody fragment by directed evolution using yeast display

Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan.

Targeted release and fractionation reveal glucuronylated and sulphated N- and O-glycans in larvae of dipteran insects.

Mechanistic aspects of catalase-peroxidase in HEME PEROXIDASES

Engineered IgG1-Fc--one fragment to bind them all.

The fucomic potential of mosquitoes: Fucosylated N-glycan epitopes and their cognate fucosyltransferases.

Mechanism of hydrogen peroxide to dioxygen oxidation by bifunctional catalase-peroxidase

Dioxygen evolution by chlorite dismutase depends on co-ordination of transiently produced hypochlorite to an oxoiron(IV) intermediate

High-resultion X-ray and neutron diffration structures and biophysical studies on a novel dimeric chlorite dismutase

Structures and thermodynamics of Fcab-antigen complexes.

From chlorite dismutase towards HemQ - the role of the proximal H-bonding network in haeme binding.

Interaction with the Redox Cofactor MYW and Functional Role of a Mobile Arginine in Eukaryotic Catalase-Peroxidase

Chemistry and Molecular Dynamics Simulations of Heme b-HemQ and Coproheme-HemQ.

Impact of heme-protein crosslinking on catalysis of prokaryotic and eukaryotic peroxidases

Chlorite dismutase: distal heme environment and its role in irreversible enzyme inactivation

Coproheme and HemQ

Influence of covalent heme to protein bonds in redox reactions of Lyngbia peroxidase

Two redox active cofactors are essential for the catalatic activity of catalase-peroxidases

Human peroxidasin 1: A multidomain heme peroxidase

Dye-decolorizing peroxidases – a new heme-peroxidase family with ancient roots

Expression, purification and characterization of monomeric IgA1 and IgA2 directed against HER2 in Nicotiana benthamiana

Hydrogen peroxide-mediated conversion of coproheme to heme b by HemQ-lessons from the first crystal structure and kinetic studies.

Pre-steady-state Kinetics Reveal the Substrate Specificity and Mechanism of Halide Oxidation of Truncated Human Peroxidasin 1.

Dye decolorizing peroxidases - A new heme-peroxidase family with ancient roots

Structure of human promyeloperoxidase (proMPO) and the role of the propeptide in processing and maturation.

Fcab-HER2 Interaction: a Ménage à Trois. Lessons from X-Ray and Solution Studies.

Exploring Site-Specific N-Glycosylation of HEK293 and Plant-Produced Human IgA Isotypes.

Posttranslational Modification of Heme b in a Bacterial Peroxidase: The Role of Heme to Protein Ester Bonds in Ligand Binding and Catalysis.

Two-faced Fcab prevents polymerization with VEGF and reveals thermodynamics and the 2.15 Å crystal structure of the complex.

Roles of the distal Asp-Arg pair in compound I formation of K. pneumoniae dye-decolorizing peroxidase

Biochemical and in-vivo investigations of a peroxidase from the social amoeba Dictyostelium discoideum